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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #29072531 -


When yall PC these cryovials, do you leave the caps loose or tighten them?


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The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 3
    #29072534 -

Loose.

And your avatar always makes me smile. :grin:

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29072539 -

Axman said:

When yall PC these cryovials, do you leave the caps loose or tighten them?


cryovials are ok but the distilled water will actually leech minerals from the wedge. i much prefer lc storage in those little vials. there is also the chance of air bubble, be sure not to underfill them, could lead to dessication.


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 2
    #29072540 -

but in t your case leave the cap only slightly backed off. it doesn't take a lot


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #29072546 -

fiddle_head said:
cryovials are ok but the distilled water will actually leech minerals from the wedge. i much prefer lc storage in those little vials. there is also the chance of air bubble, be sure not to underfill them, could lead to dessication.


I'm gonna run Mycoplex's Cryovials I got a set of 100 for like 14 bucks so I figured why not try it out. I'm going for agar wedge stasis storage so dessication isn't a huge concern because it would still be reviveable later, even if it dried out no?


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 2
    #29072550 -

I find the worst that happens if a wedge floats to the top is you get a little mat of mycelium on top. I just shake it and use it.

I also always put two wedges in. Maybe it results in a little extra nutrition for them to go through but it doesn't seem to matter. It does give a little peace of mind since one of those wedges will definitely be submerged, and if the first one doesn't recover (hasn't happened to me yet), you've got another shot from the same vial.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29072560 -

yep they're fine. what i was saying is watch out for air bubbles. sure you can revive it if it dries out, but we're going for long-term storage aren't we? lc is superior. mycoplex's cryovials are fine.


--------------------
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⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #29072572 -

Hi all,


(PF Tek + sgfc)
I have one, single large pin, about 1 - 1.5 inches long. There are a couple of tiny primordia/pins coming in when I look closely.

My question is: should I pick the large pin early to avoid stunting the rest? I've read old threads where this is recommended but I'm not sure if this is common practice now.

Thanks

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Re: Ask Quick Questions, Get Quick Answers [Re: yuuch] * 1
    #29072592 -

yuuch said:
Hi all,


(PF Tek + sgfc)
I have one, single large pin, about 1 - 1.5 inches long. There are a couple of tiny primordia/pins coming in when I look closely.

My question is: should I pick the large pin early to avoid stunting the rest? I've read old threads where this is recommended but I'm not sure if this is common practice now.

Thanks


See Water Tub Tek for updated PF cake fruiting methods.

As far as harvesting, you can harvest the large pin when the veil breaks if you would like. There's no reason to harvest it earlier than that though.

Also, try to keep your sights on the more recent threads as we've learned and evolved a LOT since this site started.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29072758 -

I'm still having a hard time not buying autosampler vials instead of cryovials :P everyones told me its unecessary but It feels like it would be so much more professional to have a built in SHIP. I'll probably invest in both because I'm hoping to sell edible and medicinal cultures like lions mane later this year and the pre SHIP'd containers would just look more professional and fool proof for customers.

so I'll ask in here too, has anyone in here already tried autosampler vials ?


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Every day is a gift

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 3
    #29072763 -

Henry Swanson said:
Loose.

And your avatar always makes me smile. :grin:


Same here, have you clicked on it? :myfaceismelting:

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29072767 -

I have, tried to get a better look :rofl:

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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29072876 -

So quick question this is my very first grows its Jack frost in a shoebox it was a aio bag that I broke up when it finished colonizing and moved to box it has been consolidating since the 22nd should I crack the lid or wait until I see pins to do so also how is it looking so far I'm not sure about that one spot in the corner that hadn't fully consolidated or is that anything to worry about.













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Re: Ask Quick Questions, Get Quick Answers [Re: INoSkopedJFK] * 1
    #29073179 -

Let's say you had an invitro grow with a 100% contam free pin you plucked out and put it on a small puck of RGS that was perfectly hydrated (another invitro PP5) and you did this entire procedure perfectly clean, would the pin colonize the jar and eventually create other pins?

I understand the contamination risk, I'm just wondering if the mycellium from the pin has the potential to colonize a small grain puck.


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“Order is needed by the ignorant but it takes a genius to master chaos.”
― Einstein


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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca] * 3
    #29073185 -

Yeah it probably will. It’s not really that risky. Not any riskier than an agar to agar transfer.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 3
    #29073199 -

Harvest time?



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #29073243 -

So much information here...and so many "this is the most current version of xxx", it is hard to tell.
Anyway, my question is about fruiting PF cakes as described in "updated PF Tek Guide 2023 Edition".
I have dunked and rolled my cakes in vermiculite as described in step six, and I'm ready to put them in the fruiting chamber.
My question - should I use the shotgun fruiting chamber according to Spitball Jedi's tek, or can I use Pastywhyte's EZ Dialed Monotub, which seems to be the more up-to-date version of the "best" monotub?

Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: phaques] * 4
    #29073254 -

Water Tub is what I'd go with.  Perlite sucks.


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Ask Quick Questions, Get Quick Answers [Re: phaques]
    #29073255 -

Water tub.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29073256 -

Jinx:stoned:


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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