3.A.M said:

fahtster said:

Finally caved


Trying the vabiooth autoclaveable dishes..  figured I’d start small just in case I didn’t like it (I’m sure I will.. the possibilities are already running wild in my brain).  The first time was pretty uncomfortable tho but that might be because I poured the jars first and getting the lids and shit situated wasn’t fun but the actual plates went pretty smoothly but I completely didn’t even think about the surface being level.  When I started pouring and all the agar went to the top, I kinda freaked out   I’ll fix that tho

Big thanks to Milkboy and his thread and holding my hand a bit.. pretty much followed that to the T.  Stipe and his vids and psilicon and his Thread

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We’re all about to get seriously humbled aren’t we.

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Huskies said:

Coolio. Does it look like a healthy plate? Not sure how tomentose these are meant to be

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Radamanto said:

I actually did that once with some leftover agar in a tupperware haha. It got full of nasty shit, specially green mold which I think was trichoderma. That nasty mold is all around my place and usually infects my pf cakes before they can give a second flush.

That’s why the concept of enmeshed mold is so strange to me. The myc looks a lot like cube myc but “weird” and not like any other contam I’ve grown voluntarily or by accident

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cronicr said:

Are u knocking your wedges to try get them Centered ? That's way too many satellite colonies

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Yes i am remember i try to center them but some don't the

Like this one with 3 pieces of Agar

polyflakes said:

Took a transfer from it but not bad looking for MS germ plate. Nats.. single drop from MS syringe.

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Radamanto said:

This is a T4 from spore. I don't have a picture of my T3 right now, but here is the T2 of this culture line.




T3 looked better, slightly more rhizo and stronger, thicker threads. Just for comparison, this is a T3 from a different  culture line, but the T3 from which I transferred the blue plate in the previous post looked almost like this but a bit better.



I’m cleaning clones of my best pf cake flushes in brf pucks right now, I also have some cakes ready to harvest soon, would you recommend to just toss the line with the enmeshed mold and start again from those other options?

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fahtster said:

thats too much pressure.  Not gonna try and reinvent the wheel here… I just wanna clean up my cultures and clone quickly

I’m not gonna dip my stem butt in peroxide before pulling it apart so I could clone in the flow without shit blowing all over my clean tissue and using a syringe that I bent the tiny needle tip in a little hook to scrape the smallest tissue piece off easily

Actually I did do those things lol. Idk how well it worked yet (except for the syringe scraper, that shit was fast af and it comes with a built-in water cooler)



I tried a scalpel at first but the tissue wouldn’t come off the blade easily and I had to wait for the damn thing to cool and I didn’t know if it was cool enough so it didn’t kill my tissue transfer.  Tissue was super easy to get off the needle… just twist it so the tip went in the agar but the tissue stayed

When I get a free month, I’ll read through this whole thread and see what tricks ppl are using

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