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Re: Ask Quick Questions, Get Quick Answers [Re: Soloist]
    #28798262 -

Soloist said:
noleboarder said:
Quick Question: What do I do in the in between time where I don't want to start a new grow?

I have like 3 diff grows going right now and don't really have the time to start anymore that I'll be able to harvest before I leave, so what do y'all recommend? I was thinking about trying to get some clones on agar once my grows start fruiting, but is there anything else i should be prioritizing?



Prioritize everyday life haha.
Or maybe get into Trichocereus cactus.
I agree with HBO though, Cannabis is always good too..



:whathesaid:


--------------------
Question everything, it's very responsive.

culture storage

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Re: Ask Quick Questions, Get Quick Answers [Re: morningWood]
    #28798377 -

morningWood said:
How long should I be letting my flowhood run before opening stuff up??



If electricity bill is of no concern one can leave it on 24/7 to essentially "scrub" the air at all times

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Re: Ask Quick Questions, Get Quick Answers [Re: dcell]
    #28798382 -

dcell said:
morningWood said:
How long should I be letting my flowhood run before opening stuff up??



If electricity bill is of no concern one can leave it on 24/7 to essentially "scrub" the air at all times




I think it would be nice to have it rigged to a outlet attached to a light switch. So you could turn on the room have the fan start running leave that room for maybe 10 minutes. Come back & that room would be clean. Of course this would all depend on the rooms dimensions and the BTU of the fan though.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #28798390 -

GenesisCorrupted said:
dcell said:
morningWood said:
How long should I be letting my flowhood run before opening stuff up??



If electricity bill is of no concern one can leave it on 24/7 to essentially "scrub" the air at all times




I think it would be nice to have it rigged to a outlet attached to a light switch. So you could turn on the room have the fan start running leave that room for maybe 10 minutes. Come back & that room would be clean. Of course this would all depend on the rooms dimensions and the BTU of the fan though.



It is actually very nice I have this exact setup for my home made hepa filter that filters the air quality before entering the fruiting room.  It's connected to a high powered blower so it moves alot of air through the filter and creates a nice turbulent air flow in the small room which then gets recirculated and refiltered.  Probably overkill but I like it.

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #28798549 -

SupaThaRipper said:
Why put it in a syringe?




really the only answer I can I could give would be for profit, you can turn 1 print into several syringes and sell them... I've given hundreds of prints away for free here

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Re: Ask Quick Questions, Get Quick Answers [Re: dcell] * 1
    #28798554 -

You'll have to replace the pre filter and hepa regularly if you leave it on 24/7.

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Re: Ask Quick Questions, Get Quick Answers [Re: Leftfield420] * 3
    #28798565 -

Leftfield420 said:
SupaThaRipper said:
Why put it in a syringe?




really the only answer I can I could give would be for profit, you can turn 1 print into several syringes and sell them... I've given hundreds of prints away for free here



Putting spores in a syringe keeps them hydrated if you need/want to store them long term.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] * 1
    #28798573 -

ansr said:
Mackload said:
ansr said:
The first print I made failed I think. It was very light and had water drops on the print but I let it dry and tried to get it into a syringe but that was easier said than done. It seems like the spores got stuck to the plastic on the inside of the syringe. :thumbdown:
I made some jars just to try and see if it works anyway.


This is my second print, looks much better. Now I need to learn how to properly get it into a syringe.




Alternately, you could just scrape a little on agar



I started this hobby less than two months ago and I started by failing a lot trying to work with agar. :rolleyes:

I only had a spore syringe and tried to get mycelium going on agar and it did not go well. I know I need to learn it, it seems fun, but I'm not confident.

It seems tricky to get rid of condensation without contaminating the plates without a laminar flow hood.



Bro, if I can do it, you can do it, lol. I don't think you have to even worry about condensation, and a SAB is plenty good enough for starters, I believe.  :thumbup:


--------------------
Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: Mackload] * 6
    #28798718 -

Mackload said:
ansr said:
The first print I made failed I think. It was very light and had water drops on the print but I let it dry and tried to get it into a syringe but that was easier said than done. It seems like the spores got stuck to the plastic on the inside of the syringe. :thumbdown:
I made some jars just to try and see if it works anyway.


This is my second print, looks much better. Now I need to learn how to properly get it into a syringe.




Alternately, you could just scrape a little on agar



:spank:
Streak, don't scrape!


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: thirdeyewild]
    #28798755 -

Does anyone know of any species that DONT do well in a LC or are negatively impacted by the extra moisture in the jar after grain inoculation?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #28798768 -

Bro I'm glad I listened to you. They fat



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Re: Ask Quick Questions, Get Quick Answers [Re: noleboarder]
    #28798790 -

My goal is to always print every grow, and now I've started cloning on agar anything that's healthy/clone-worthy.

So yeah, start some plates and transfer them when you get back...

Just pulled these today:


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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 5
    #28798810 -

Smellyhobbit said:
Does anyone know of any species that DONT do well in a LC or are negatively impacted by the extra moisture in the jar after grain inoculation?



Cordisporae (neoxalapensis, fagicola, yungensis, etc) species are slow and can die in LC if not stirred gently, or if shaken too hard.

They also don't take well to anything with too many nutrients it seems, like grain. So LC to grain with them frequently fails. I suspect it's due to too low of available moisture on the surface of the grain, so they give up before they make it inside.

If you go agar to grain, they visibly suck the moisture from the puck of agar and use it to spread.

Here are neoxalapensis and fagicola sucking out the moisture and using it to spread on grain that is probably too dry. (Oats and millet). You can also see where it contracts the grains together and the puck has sunken inward.




So maybe I'm not answering your question, certainly not a quick answer :rofl:, because they all do ok in low nute LC but if you shoot LC into grain with these they often just act as if you didn't inoculate at all.

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Re: Ask Quick Questions, Get Quick Answers [Re: wavyedge] * 2
    #28798832 -

No that’s definitely an answer to my question.

I’m asking about both factors: will it colonize LC, and will the LC to grain performance suffer?

Good information, thank you 👍


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #28798853 -

I think Angulospora is the reigning champion of sensitivity here. Cordispora section are a close runner up.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28799002 -

DERRAYLD said:
You'll have to replace the pre filter and hepa regularly if you leave it on 24/7.



This is absolutely true.

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Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one]
    #28799010 -

I want to take a sample of some monsters I grew, but the caps have opened. Is it too late to "clone" some of the stem?


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Re: Ask Quick Questions, Get Quick Answers [Re: TriangleTriangle] * 5
    #28799026 -

It's irrelevant if the caps are open or closed. What you want is a sample of inner sterile tissue that was never in contact with the atmosphere.

tek


--------------------
“All this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999] * 1
    #28799297 -

Thanks Gleyck!

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Re: Ask Quick Questions, Get Quick Answers [Re: Leftfield420]
    #28799332 -

Mackload said:
ansr said:
Mackload said:
ansr said:
The first print I made failed I think. It was very light and had water drops on the print but I let it dry and tried to get it into a syringe but that was easier said than done. It seems like the spores got stuck to the plastic on the inside of the syringe. :thumbdown:
I made some jars just to try and see if it works anyway.


This is my second print, looks much better. Now I need to learn how to properly get it into a syringe.




Alternately, you could just scrape a little on agar



I started this hobby less than two months ago and I started by failing a lot trying to work with agar. :rolleyes:

I only had a spore syringe and tried to get mycelium going on agar and it did not go well. I know I need to learn it, it seems fun, but I'm not confident.

It seems tricky to get rid of condensation without contaminating the plates without a laminar flow hood.



Bro, if I can do it, you can do it, lol. I don't think you have to even worry about condensation, and a SAB is plenty good enough for starters, I believe.  :thumbup:



I bought more dishes so I will try again soon. Can you let them cool down in a SAB without the lids or will that contaminate them?


Leftfield420 said:
SupaThaRipper said:
Why put it in a syringe?




really the only answer I can I could give would be for profit, you can turn 1 print into several syringes and sell them... I've given hundreds of prints away for free here



I only got one syringe and used it all when I tried different things to get going. I failed with everything except PF tek so I'm doing more of that for now, that's why I want to put it in a syringe. :grin:

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Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag


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