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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/05/11
Posts: 10,573
Trusted Cultivator
Frank's Proper Cloning Tek * 28
    #17784996 - 02/11/13 08:34 PM (9 years, 4 months ago)

This is part of my series on how I get things done. I hope it helps!

The easiest way to get a good set of genetics is to clone an ideal looking fruit or cluster from a multispore grow. This process is very simple, and the way I do it is essentially the same way that RR does it.

Some people will try to clone into LC or using a needle, etc. This will almost always fail. Make sure to use agar. Since fruiting is done in open air, you can always assume the fruit is covered in contaminants. This is doubly true for cloning wild mushrooms.

Even though you'll be taking a cut of virgin mycelium that has never been exposed to open air, it is very common to need to transfer your mycelium away from contaminants on the first dish.

Since a flowhood can potentially blow contaminants around from the fruit you are cloning, I recommend working in a still air box.


Start by wearing tyvek sleeves and disposable gloves. Make sure to work quickly and carefully inside the still air box. Use good sterile technique.

Flame sterilize your scalpel and set it down in the still air box where it won't be disturbed. I place it on my receiving petri dish. Do not set it on the floor of your SAB.

Take the mushroom (this is a golden oyster) and pinch it at the base. Rip it down the middle. Do not cut into the stem with your scalpel or you will force contaminants down into it.



Holding part of the fruit with one hand, use your scalpel to gently scrape at the center of the stem, near the base. Try to get a small piece on to the blade. As RR says, a scraping motion will be easier than trying to slice a specific piece.


Once you have a small piece of tissue on the end of your scalpel, transfer it to the agar. It may take a bit of effort to get it off the blade :shrug:


Take as many pieces of tissue as you want to clone. You can flame sterilize between each cut, or just flame sterilize between each petri dish or fruit. I use 3 section dishes so I flame sterilize every three cuts.

That's all there is to it. Wrap the petri dish and let it colonize. Transfer healthy mycelium away from any contaminants as soon as they appear!

Some fruits will give you an isolate right off the bat, but a lot of mushrooms are actually composites of multiple genetics. However, I have seen TCs say that the original clone sectors perform better together than as an isolate of each. So it's up to you if you decide to isolate the clone further :thumbup:


:cheers:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (03/04/13 12:17 AM)


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Offlinecherry_darling
i smell horseshit!
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Registered: 12/08/12
Posts: 589
Loc: Planet Terror (ohio)
Last seen: 8 years, 11 months
Re: Frank's Proper Cloning Tek [Re: FrankHorrigan]
    #17785025 - 02/11/13 08:37 PM (9 years, 4 months ago)

>bookmarked<

thanks for writing this up, you have been of infinite help to me personally, as I'm sure many others! :mushroom2:


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InvisibleJoeMama1992
Asleep at the Wheel


Registered: 12/27/11
Posts: 889
Re: Frank's Proper Cloning Tek [Re: cherry_darling] * 1
    #17785069 - 02/11/13 08:45 PM (9 years, 4 months ago)

:nothingtoadd:
*Bookmarked*


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InvisibleDeadkndys420
The Psychedelic Messiah
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Registered: 05/08/12
Posts: 4,405
Loc: In the land of ooo
Re: Frank's Proper Cloning Tek [Re: JoeMama1992]
    #17785213 - 02/11/13 09:06 PM (9 years, 4 months ago)

Quote:

cherry_darling said:
>bookmarked<




Quote:

JoeMama1992 said:
*Bookmarked*




--------------------
Almond Flour said:
Thats right. I take it up the ass on a daily. It feels great to finally let this out :gay:

How to use PGP encryption
The Stoner's Cookbook


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/05/11
Posts: 10,573
Trusted Cultivator
Re: Frank's Proper Cloning Tek [Re: Deadkndys420] * 1
    #17788603 - 02/12/13 01:01 PM (9 years, 4 months ago)

:thanx:
I thought the forums could use a good cloning write up.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinewire5
Hippie Born too Late
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Registered: 04/05/11
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Re: Frank's Proper Cloning Tek [Re: FrankHorrigan]
    #17788611 - 02/12/13 01:02 PM (9 years, 4 months ago)

:awesomenod::popcorn:


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OfflinePirax
Self Employed Pharmacist

Registered: 11/26/12
Posts: 388
Loc: Boregon
Last seen: 5 years, 10 months
Re: Frank's Proper Cloning Tek [Re: wire5]
    #17788648 - 02/12/13 01:09 PM (9 years, 4 months ago)

Nice tek, you posted this the day my caps opened up and my MEA arrived, nice timing :thumbup:


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OfflineTheCyndicate
Conglomerate
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Registered: 10/16/11
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Re: Frank's Proper Cloning Tek [Re: Pirax]
    #17788821 - 02/12/13 01:44 PM (9 years, 4 months ago)

You know what I'm gonna say frank!

Cyn


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InvisibleHypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
Re: Frank's Proper Cloning Tek [Re: TheCyndicate]
    #17888071 - 03/01/13 09:01 PM (9 years, 3 months ago)

THANK YOU FRANK.  I have to say that the awesomest thing about this forum, is that whenever I have a question, or are about to try something risky, someone posts the correct method a few days prior.

This is a great writeup, and I will CERTAINLY be using it in the very near future.  I can assume it would work with Doc34's cheap-skate agar tek? 

http://www.shroomery.org/8514/Agar-substitute


Thanks Frank!


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Invisiblegizmo1
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Registered: 06/15/11
Posts: 3,799
Loc: FREEDOM Flag
Re: Frank's Proper Cloning Tek [Re: HypnotoadCroaked]
    #17888594 - 03/01/13 10:24 PM (9 years, 3 months ago)

I have been taking my tissue samples straight to grain risky I know but hasn't failed me yet(knock on wood).


--------------------
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Edited by gizmo1 (03/01/13 10:51 PM)


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Invisibleb plus
poppin' veils

Registered: 10/13/12
Posts: 928
Re: Frank's Proper Cloning Tek [Re: gizmo1]
    #17888667 - 03/01/13 10:34 PM (9 years, 3 months ago)

gizmo- try this:

Take the tissue sample, and toss into a newly opened ziploc bag. Leave for 2-3 weeks. Don't worry about holes for GE or anything. Acutally compress almost all of the air out of the bag so only a very small amount is left. Also make sure the sample is a little smaller than the size or a pea or pencil eraser or I find that it tends to dry out. After a week or two, the tissue sample is covered in fluffy white myc. Almost like a petri, without the agar, or the dish... just a bag.. I've had pieces that clearly show signs of bacterial contam, but I very rarely have contamed peices. Most of the time they just dry out too quickly before myceium begins to grow.

Anyways while in a SAB, transfer it from the bag into the grain jar... you know the drill:thumbup:


--------------------
:mushroom2:-Reishi Grow-:mushroom2:
:mushroom2:-Grey Oyster Grow-:mushroom2:


Edited by b plus (03/01/13 10:35 PM)


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OfflineMycospore
Male User Gallery


Registered: 09/24/11
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Re: Frank's Proper Cloning Tek [Re: b plus]
    #17889022 - 03/01/13 11:17 PM (9 years, 3 months ago)

:thumbup:


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/05/11
Posts: 10,573
Trusted Cultivator
Re: Frank's Proper Cloning Tek [Re: b plus]
    #17896744 - 03/03/13 01:04 PM (9 years, 3 months ago)

Quote:

TheCyndicate said:
You know what I'm gonna say frank!

Cyn




Oh I do! :lol:
Where ya been lately, Cyn?

Quote:

Whippy said:
THANK YOU FRANK.  I have to say that the awesomest thing about this forum, is that whenever I have a question, or are about to try something risky, someone posts the correct method a few days prior.

This is a great writeup, and I will CERTAINLY be using it in the very near future.  I can assume it would work with Doc34's cheap-skate agar tek? 

http://www.shroomery.org/8514/Agar-substitute


Thanks Frank!




:thanx:
I'm glad I could help!

I am sure it could be used with agar subs, but I would just grab a sleeve of petris and some malt extract agar and try it for real :shrug:

Quote:

b plus said:
try this




:smbfacepalm:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinetripdawg420
low life with no life
Male


Registered: 02/03/09
Posts: 6,974
Loc: illinois
Last seen: 10 hours, 37 minutes
Re: Frank's Proper Cloning Tek [Re: FrankHorrigan]
    #17896973 - 03/03/13 01:58 PM (9 years, 3 months ago)

i see a TC tag in your future :pimp: you pretty much summed it up :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineTheCyndicate
Conglomerate
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Registered: 10/16/11
Posts: 1,195
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Re: Frank's Proper Cloning Tek [Re: tripdawg420]
    #17898326 - 03/03/13 06:31 PM (9 years, 3 months ago)

Frank.

Taking a break man . I love our hobby with a passion! !. But it was consuming me and all of my free time. Desided to spend a little more time with my lovely wife and little ones . And consntrate on whats important. . Dont worry I'm still here . Not going anywhere . Just chillin out for a while!!

Cyn


Edited by TheCyndicate (03/06/13 08:58 PM)


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/05/11
Posts: 10,573
Trusted Cultivator
Re: Frank's Proper Cloning Tek [Re: TheCyndicate]
    #17900658 - 03/04/13 02:06 AM (9 years, 3 months ago)

Quote:

tripdawg420 said:
i see a TC tag in your future :pimp: you pretty much summed it up :thumbup:




:thanx:
I'm just glad I can help out the community that taught me almost everything I know! :lol:

Quote:

TheCyndicate said:
Frank.

Taking a break man . I love your hobby with a passion! !. But it was consuming me and all of my free time. Desided to spend a little more time with my lovely wife and little ones . And consntrate on whats important. . Dont worry I'm still here . Not going anywhere . Just chillin out for a while!!

Cyn




Good to hear things are well in your world, man.
It is a time consuming hobby. People ask how much I actually work when I'm sitting at home and I tell them "6-8 hour days."
They think I'm lying, but I'm not :facepalm:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
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Re: Frank's Proper Cloning Tek [Re: FrankHorrigan] * 1
    #17900674 - 03/04/13 02:10 AM (9 years, 3 months ago)

Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/05/11
Posts: 10,573
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Re: Frank's Proper Cloning Tek [Re: PussyFart]
    #17900682 - 03/04/13 02:11 AM (9 years, 3 months ago)

Quote:

Notahacker420 said:
Yes, but I try think of it as productive entertainment, as opposed to work, but after a while, it becomes work, then a pain, then an obsessive passion where it's only driving force is you, lol.




Agreed. I love every second of it.
They call it "work," I just let them think of it that way :lol:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisibleannnon
KillerClown

Registered: 05/13/13
Posts: 86
Loc: Between Heaven/Hell
Re: Frank's Proper Cloning Tek [Re: FrankHorrigan]
    #18261386 - 05/14/13 02:06 AM (9 years, 1 month ago)

Hay Frank, can you please explain to me why you would grab your scraping of tissue from the base and not the middle or head? Also

I have tried a tissue to grain and it was painfully slow so instead of using one scraping I used ten per jar. The myc popped up in many areas quickly, not in every jar though and that is why I am asking what I am.

Thanks mucho in advance my good man!


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Offlineitsaconspiracy
governments worst nightmare
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Registered: 03/26/13
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Re: Frank's Proper Cloning Tek [Re: annnon]
    #18261403 - 05/14/13 02:10 AM (9 years, 1 month ago)

:asianofapproval: i like it:thumbup: and why didn't you use a Psilocybe cubensis


--------------------
("i'm not a criminal i just like stuff thats not mine")
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