Frank's Proper Cloning Tek - Mushroom Cultivation

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Best Posts Active Topics Galleries FAQ User List Chat Store Random Growery

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

Bulk Cannabis Seeds

Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder

Buy Kratom Extract, Kratom Powder For Sale

Red Vein Kratom

Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post

Pages: 1 | 2 | 3 | 4 | Next > [ show all ]

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

FrankHorrigan
The Inquisition

Registered: 01/05/11
Posts: 10,573

Frank's Proper Cloning Tek

28
#17784996 - 02/11/13 08:34 PM (9 years, 4 months ago)

This is part of my series on how I get things done. I hope it helps!

The easiest way to get a good set of genetics is to clone an ideal looking fruit or cluster from a multispore grow. This process is very simple, and the way I do it is essentially the same way that RR does it.

Some people will try to clone into LC or using a needle, etc. This will almost always fail. Make sure to use agar. Since fruiting is done in open air, you can always assume the fruit is covered in contaminants. This is doubly true for cloning wild mushrooms.

Even though you'll be taking a cut of virgin mycelium that has never been exposed to open air, it is very common to need to transfer your mycelium away from contaminants on the first dish.

Since a flowhood can potentially blow contaminants around from the fruit you are cloning, I recommend working in a still air box.

Start by wearing tyvek sleeves and disposable gloves. Make sure to work quickly and carefully inside the still air box. Use good sterile technique.

Flame sterilize your scalpel and set it down in the still air box where it won't be disturbed. I place it on my receiving petri dish. Do not set it on the floor of your SAB.

Take the mushroom (this is a golden oyster) and pinch it at the base. Rip it down the middle. Do not cut into the stem with your scalpel or you will force contaminants down into it.

Holding part of the fruit with one hand, use your scalpel to gently scrape at the center of the stem, near the base. Try to get a small piece on to the blade. As RR says, a scraping motion will be easier than trying to slice a specific piece.

Once you have a small piece of tissue on the end of your scalpel, transfer it to the agar. It may take a bit of effort to get it off the blade :shrug:

Take as many pieces of tissue as you want to clone. You can flame sterilize between each cut, or just flame sterilize between each petri dish or fruit. I use 3 section dishes so I flame sterilize every three cuts.

That's all there is to it. Wrap the petri dish and let it colonize. Transfer healthy mycelium away from any contaminants as soon as they appear!

Some fruits will give you an isolate right off the bat, but a lot of mushrooms are actually composites of multiple genetics. However, I have seen TCs say that the original clone sectors perform better together than as an isolate of each. So it's up to you if you decide to isolate the clone further :thumbup:

--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (03/04/13 12:17 AM)

Extras: