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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Zav3r] * 2
    #28473489 -

Zav3r said:
What to do with FAE and misting after the first flush? Do I keep regularly misting? I'm assuming that the cake is well hydrated after the dunk and hydration but not quite sure.

What about FAE? thanks guys


You never mist regularly. If you have to you’re getting too much FAE. You only mist when it’s dry. If your tote is dialed and you hydrate your sub, you shouldn’t have to mist much if at all.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #28473518 -

Understood

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Re: Ask Quick Questions, Get Quick Answers [Re: Zav3r]
    #28473534 -

Is this utterly special thus worth cloning?



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28473542 -

Huskies said:
Is this utterly special thus worth cloning?




Do you like it? If so, clone it!


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28473598 -

While I am not 100% sure, I think that for the last two tubs I harvested, I had packed the sub tight, then applied a top layer of pure coir, then packed that pretty tight.

The very top layer felt dry, and pulled up bigger chunks than usual.

Is this due to the top layer not being fully colonized since it's doesn't have grains directly in it? Or is it just that the tub and coir was overall a bit too dry?

Someone mentioned this happening when they packed their subs, so maybe neglecting the top layer of just coir is better?

What even is the purpose of a pure coir top layer?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #28473631 -

Hi everyone,
Is there any place for CaCO3 in mush Cultivation, or has it been put out to pasture same as gypsum?

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #28473638 -

RoscoeReturns said:
thcee said:
Is keeping an air purifier going 24/7 in the same room helping/harming at all?

My guess is no but just curious.


No.


Thought so, thank you.


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:sporedrop:🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 :skittles2:


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Re: Ask Quick Questions, Get Quick Answers [Re: drVetker] * 1
    #28473639 -

drVetker said:
Hi everyone,
Is there any place for CaCO3 in mush Cultivation, or has it been put out to pasture same as gypsum?


Hydrated lime can be used to "cold pasteurize" wood or straw substrates. This  will apply mostly to gourmets. You could pasteurize large batches of straw or woodchips for outdoor beds, straw logs, etc.

Outdoor beds for: ovoid, cyan, allenii, or azures, could benefit from cold pasteurization. Cubes can be grown on straw logs, but that's about as useful as it can be for cubensis.

Lime is often used in casing recipes to adjust ph. Peat/lime casings are used for species other than cubes, but, you can use it for PE if you're into making casing material, however these materials are more often reserved for panaeolus.

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Re: Ask Quick Questions, Get Quick Answers [Re: drVetker]
    #28473640 -

I haven't heard anyone talking about adding calcium since I first was learning around in 2012.

Most important thing for good yields and potency are clean spawn and selecting for the best growth on agar. We are overall past the age of over-complicating things: clean spawn +coir = happy.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #28473656 -

Stipe-n Cap said:
drVetker said:
Hi everyone,
Is there any place for CaCO3 in mush Cultivation, or has it been put out to pasture same as gypsum?


Hydrated lime can be used to "cold pasteurize" wood or straw substrates. This  will apply mostly to gourmets. You could pasteurize large batches of straw or woodchips for outdoor beds, straw logs, etc.

Outdoor beds for: ovoid, cyan, allenii, or azures, could benefit from cold pasteurization. Cubes can be grown on straw logs, but that's about as useful as it can be for cubensis.



Ok, this is all good info.
So adding a half a teaspoon to grain when doing the prep is pointless. Good to know.
But if it can help cold pasteurize straw wouldn't it stand to reason that it would make our grain then more resistant to contamination spreading when inoculating it with agar etc. (Opening closing the bottle, having the lid 1/4 inch opened for FAE) ?

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Re: Ask Quick Questions, Get Quick Answers [Re: drVetker]
    #28473663 -

Grain is sterilized, not used in open air. Pasteurization and sterilization are way different


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: drVetker] * 1
    #28473664 -

No, not at all. Lime is a chemical agent which alters ph, the sterilization cycle will not leave any living organism to be affected by thw lime. The autoclave dentures proteins in microorganisms, destroying them entirely.

The addition of lime will have no effect whatsoever on sterility, but may negatively effect your culture. If you contaminate via sloppy technique, no amount of lime or any other agent will correct or mitigate the mistake. Technique and clean inoculum are paramount, there's no short cut or safety net.

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #28473666 -

Stipe-n Cap said:
No, not at all. Lime is a chemical agent which alters ph, the sterilization cycle will not leave any living organism to be affected by thw lime. The autoclave dentures proteins in microorganisms, destroying them entirely.

The addition of lime will have no effect whatsoever on sterility, but may negatively effect your culture.


I understand both of you, thank you for replying. The sterilization and pasteurization difference is clear, the idea was going more in the direction of it helping with inadvertent exposure to contam during e.g. inoculation, meaning after it's been sterilized already.

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Re: Ask Quick Questions, Get Quick Answers [Re: drVetker]
    #28473670 -

Read the edit.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28473673 -

Good spore company ?

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #28473674 -

Stipe-n Cap said:
Read the edit.


Got it. Thank you.

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Re: Ask Quick Questions, Get Quick Answers [Re: drVetker]
    #28473725 -

Speaking of additives being a detriment, I recently made low/no-prep oats for the first time and I added some gypsum to the water help with clumping.
But I think I added way too much. I only made a few jars, but I noticed the first two I inoculated, one with LC one with Agar, have been extremely slow to colonize and the mycelium looks weaker.

Would too much gypsum/calcium cause this? Or should
I attribute it to using oats instead of my normal WBS?


--------------------





Edited by MicroMycelium (09/18/23 04:00 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium]
    #28473749 -

You probably already know this but people dont even use gypsum anymore. I used to but stopped as well.


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28473763 -

15 qt  unmodified "shoebox" spawn to bulk on 9/6 so almost 2 weeks ago. Spawn was clean as far as I could tell. Used a White Rabbit "isolated syringe" / LC that's been tested on agar. Hopefully expecting pins soon.
--The upper/middle right side has started looking a bit more matted than the rest. Could be a result of contam? Either way I'm gonna let it ride.
-- Seems like 2 weeks is a while to still not be seeing pins.



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Re: Ask Quick Questions, Get Quick Answers [Re: Chumbum]
    #28473785 -

I still use gypsum for casing, but if all you run is coir and grain it's a waste of space.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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