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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] * 3
    #28370048 -

stockw said:
I like to use a fresh one per dish when doing a2g.


:wtfno:


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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 3
    #28370062 -

Mycodemus said:
Mycodemus said:
I cut off the weirdest part of my infected tub and thought I’d fill in the space with coir. It’s been generous so far so what the hell, even though I know the contam isn’t gone for good. Hey, I got 180 wet out of it, so for my 2nd box ever I’m not displeased.

The only question is, I cut off about 2 inches along the long side, do I center the sub and put coir on both sides, or just along the one side? And should I patch the surface where it ripped a bit when harvesting?


Bumpy bump :smile:


Lmao, just fucking leave it and keep a close eye on it. Very unlikely you got all the contam out of there and very likely that you spread it around a bit more.


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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] * 1
    #28370082 -

Scemilope said:
Nah, sharp doesn't matter too much, you can buy them blunted so they won't scratch pp plates. I just like the smooth surface tension pickup and release of a fresh-ish blade.


I just hate ripping those massive chunks of ropy myc from the mother plate. Maybe it's my technique that sucks, idk :shrug:

SirPsycho said:
What are y'all cooling your scalpel for? Only time a tool needs cooling is if you're gonna use it to grab spores cause ideally you're only grabbing as few as you can (so a red hot tool might kill em but a proper nichrome loop cools almost instantly anyway), plus getting that melted agar on the surface helps the spores stick.

If you're transferring myc you don't need to cool shit. Sure, it'll probably damage the myc more than a cool scalpel but really what's a few thousand extra dead cells?


For real? Just shove it in there while it's red hot, no lube no nothing?

Fuck it why not, one less thing to fuck around with in the sab.



stockw said:
I like to use a fresh one per dish when doing a2g.


:eww:

But why ? Like why specifically grain? You could literally use a fork for that and just mash it in there, you are never seeing that wedge again.


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] * 3
    #28370083 -

Yeah Mycodemus the tub is almost definitely going to severely contam out before any new cubes have a chance to grow. If you want to leave it hanging around as a learning experience it will be good for that. Cutting contam out of a substrate isn’t really a viable solution. Just let it do its thing or toss it. Either way, start a new project πŸ‘


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:kodama:


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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 3
    #28370111 -

Smellyhobbit said:
In my hobbies I always allow myself one frivolity.


I get it. My frivolous things for my hobbies are: lure holding= rod. Reeling in the fish= reel. Getting the fish to bite= airbrush set up and paint, lure blanks and fly tying equipment. Keeping the fish on the lure= hooks. Getting to the fish= boat or kayak. Finding them fish= fish finder. Damn I got a lot of hobbies. Lol


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Re: Ask Quick Questions, Get Quick Answers [Re: Bigworm] * 1
    #28370114 -

You ain't catching sheet if you ain't hone-gry!


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Yeah, yeah.

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28370136 -

Smellyhobbit said:
Yeah Mycodemus the tub is almost definitely going to severely contam out before any new cubes have a chance to grow. If you want to leave it hanging around as a learning experience it will be good for that. Cutting contam out of a substrate isn’t really a viable solution. Just let it do its thing or toss it. Either way, start a new project πŸ‘


Other boxes annd projects are on the way, I’ll just leave it and see what happens. Thanks guys.


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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28370212 -

SirPsycho said:
What are y'all cooling your scalpel for? Only time a tool needs cooling is if you're gonna use it to grab spores cause ideally you're only grabbing as few as you can (so a red hot tool might kill em but a proper nichrome loop cools almost instantly anyway), plus getting that melted agar on the surface helps the spores stick.

If you're transferring myc you don't need to cool shit. Sure, it'll probably damage the myc more than a cool scalpel but really what's a few thousand extra dead cells?


I used to go in hot but I realized the agar melting and getting sticky was why I struggled so hard to get the transfer off my scalpel. That being said, I’m still working my way through the chefs knife scalpel blades my handle came with, and it was when I was using pasty plates and had to come in at a hard angle to clear the tall sides. Always ended up pushing the transfer further onto the blade it’s was infuriating πŸ˜‚


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Re: Ask Quick Questions, Get Quick Answers [Re: aPurpleCray0n] * 2
    #28370216 -

Cool your scalpel in the agar before you take your transfer...
Does no one else do this? I'm pretty sure we all do this.


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Re: Ask Quick Questions, Get Quick Answers [Re: aPurpleCray0n] * 2
    #28370217 -

I used to have issues with getting the transfer off when working with 8oz jars but with plates it's super easy, never had issues with the scalpel and petris

Needle nose tweezers and samples of mushroom flesh? That's always a bitch


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

Edited by SirPsycho (06/22/23 03:42 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: mushhead] * 1
    #28370220 -

mushhead said:
Cool your scalpel in the agar before you take your transfer...
Does no one else do this? I'm pretty sure we all do this.


No, I don't and you can't make me :grrr:


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] * 2
    #28370229 -

SirPsycho said:
mushhead said:
Cool your scalpel in the agar before you take your transfer...
Does no one else do this? I'm pretty sure we all do this.


No, I don't and you can't make me :grrr:


:cop2: Oh I'll make you alright. But seriously, I have no issues ever with cooling in agar a sec before taking the transfer.
I also have a wee stand inside my SAB that I put my scalpel in, it does cool there sometimes if I have to unwrap plates or something.
Otherwise IDGAF it goes in pretty hot.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Re: Ask Quick Questions, Get Quick Answers [Re: mushhead] * 1
    #28370233 -

mushhead said:
SirPsycho said:
mushhead said:
Cool your scalpel in the agar before you take your transfer...
Does no one else do this? I'm pretty sure we all do this.


No, I don't and you can't make me :grrr:


:cop2: Oh I'll make you alright. But seriously, I have no issues ever with cooling in agar a sec before taking the transfer.
I also have a wee stand inside my SAB that I put my scalpel in, it does cool there sometimes if I have to unwrap plates or something.
Otherwise IDGAF it goes in pretty hot.


I'm not saying it'll cause issues, I'm saying it's unnecessary for agar transfers.

Only time it's "necessary" is spore inoculation.

See comment near top of page.

Also, it cools while I cut the transfer piece. Dafuq
:kingcrankey:


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] * 3
    #28370236 -

Listen, y'all do you. I'm just sloppy AF tbh. My goal is to be as lax and sloppy as possible while still having acceptable failure rates (below 3%)


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] * 1
    #28370241 -

:heart: u SirPsycho
Not saying it's necessary it's just a thing you can do to cool your scalpel off if that's what you want to do you know?
Most my transfers are done pretty hot anyways, I take no chances usually.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28370244 -

Quote:
My goal is to be as lax and sloppy as possible while still having acceptable failure rates


Same.

I even inoculated my last 3 PF jars without using an SAB. Was just too lazy to get it and i heard you can get away without it.


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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] * 1
    #28370245 -

Got my first 0 shroom rating too! Moving up in life

:tryingnottodie:


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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28370249 -

Pandaskis said:
Got my first 0 shroom rating too! Moving up in life

:tryingnottodie:


Yeah it’s from that troll old account. He gave me one too.


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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis] * 1
    #28370251 -

No worries, i just gave you 5 to even it out.


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28370256 -

Yahra said:
Quote:
My goal is to be as lax and sloppy as possible while still having acceptable failure rates


Same.

I even inoculated my last 3 PF jars without using an SAB. Was just too lazy to get it and i heard you can get away without it.


Alright, real talk?

If you're working with syringes and sealed containers with injection ports of some sort, you're fine to work in open air.

They'll probably take my TC tag away for that one:lol:


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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