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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28349799 -

PanikAttak said:
Hi! New grower, here. I’ve noticed the mycelium getting yellow and even brown in some spots, the side especially concerns me. It’s PE in an unmodded shoe box with its lid off in a modded monotub. Been fanning & misting for 5ish days, the lid was on it prior to that and the mycelium used to be completely white. Are these just metabolites or is it contam? If metabolites is it ok, or is there too much or too little FAE or moisture?











I'd remove it from the modified monotub, give it a spritz to get decent surface conditions again, close the lid back up, and leave it. Shoeboxes fruit just fine with their lid on and it appears your monotub isn't dialed in.

Its also likely to be bacterial just based on how the mycelium is growing and the interesting colors on the side. You'll likely still get mushrooms, but I would fix your conditions immediately.

Welcome by the way


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28349801 -

Thank you! I’ll stop everything and only mist as needed. Hoping there was no bacterial contamination, had it on agar and then to LC, everything and the spawn looked great, it just started when I tried to induce FC, but I could have introduced something any step of the way. Learning a lot.

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Re: Ask Quick Questions, Get Quick Answers [Re: PanikAttak]
    #28349804 -

PanikAttak said:
Thank you! I’ll stop everything and only mist as needed. Hoping there was no bacterial contamination, had it on agar and then to LC, everything and the spawn looked great, it just started when I tried to induce FC, but I could have introduced something any step of the way. Learning a lot.


Got pictures of the spawn?


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: PanikAttak]
    #28349808 -

PanikAttak said:
Thank you! I’ll stop everything and only mist as needed. Hoping there was no bacterial contamination, had it on agar and then to LC, everything and the spawn looked great, it just started when I tried to induce FC, but I could have introduced something any step of the way. Learning a lot.


Would suggest just sticking with Agar for a bit before graduating to LC. LC has a lot of contam vectors, and even worse due to there being liquids involved, bacteria spreads very easily if it is contamed.


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A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
    #28349815 -

Not from the exact day I spawned to bulk for some reason but here’s the last day I did have.  Frustrated I contaminated it somewhere along the way, but I suppose it’s bound to happen. I’m still learning.




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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28349816 -

I’ll definitely stick to that for now. I figured since the agar looked clean and it looked good from the jar everything was fine, but I guess it’s just way too hard to tell.

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Re: Ask Quick Questions, Get Quick Answers [Re: PanikAttak]
    #28349819 -

PanikAttak said:
Not from the exact day I spawned to bulk for some reason but here’s the last day I did have.  Frustrated I contaminated it somewhere along the way, but I suppose it’s bound to happen. I’m still learning.






Looks slightly bacterial to me, im thinking the tub is acting like that more from poor conditions


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A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28349832 -

Yahra said:
TL;DR:
Can i spawn a single PF cake to bulk?

My last batch of PF tek jars wasn't that successful and only 2 out of 10 jars germinated.

I am thinking about how to fruit these 2 cakes.

My water tub is huge, i can easily fit 10 cakes in it so i want to try something different.

I thought of spawning each cake to bulk in some kind of a nano shoebox.

Does that make sense?


My first box which is at the end of the first flush now is from 4 PF cakes that were maybe 80-100 ml big, shredded 1:1 to bulk with coir, plus .5 on top. I’ve been posting pics if you’ve seen them. I put it in a Tupperware that is about 8x8 inches. It’s not deep enough really, but I’ve gotten a bit over 50 wet grams from it so far. Someone here told me a few weeks ago it was highly suspect for contams, but it’s delivered a harvest and for a first ever box I’m not disappointed.

Shred to bulk in a quite small container and see what happens, is my vote :smile: am a noob, so grain of salt and all that, but I’m pretty pleased. I generally refrain from giving advice at all, bc noob, but I’ve been mildly successful so far with this box, so it’s worth a try probably.


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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28349835 -

PanikAttak said:
Not from the exact day I spawned to bulk for some reason but here’s the last day I did have.  Frustrated I contaminated it somewhere along the way, but I suppose it’s bound to happen. I’m still learning.






Looks way better than I expected but still looks a bit off to me.

I wouldn't stress though. Just let it play out. The only thing on the tub that really made me think that was the dense bright white patch on the left and the inconsistent colonization. That could be a result of improperly mixed substrate and grains or it could just be early and it'll fill out a suppose.

You're doing great so far :thumbup:


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28349836 -

Went ahead and put the lid back on and took it out of the monotub. Hopefully it’s salvageable then. I guess even with a little bacterial contam, they can still fruit and be safe?

Just took some photos from right before I put the lid back on and boy, it does it look to happy.




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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
    #28349841 -

Thanks! I hope it’s still salvageable. I thought part of the issue was that I made the pseudo casing layer a bit too thick, because underneath that it all appeared to colonize evenly from the sides, but I probably didn’t mix it that well either since it was my first try at this. I think I really jumped the gun on this and tried to fruit it before it was ready.

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Re: Ask Quick Questions, Get Quick Answers [Re: PanikAttak] * 1
    #28349843 -

PanikAttak said:
Went ahead and put the lid back on and took it out of the monotub. Hopefully it’s salvageable then. I guess even with a little bacterial contam, they can still fruit and be safe?

Just took some photos from right before I put the lid back on and boy, it does it look to happy.






Yeah, saying a tub is bacterial or contaminated doesn't mean shit other than it may not produce as well as a clean tub and it has a higher chance of going south completely by getting a tub ending contamination like trich. I'd say a majority of slightly bacterial tubs end up pumping out shrooms anyways.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: PanikAttak] * 1
    #28349847 -

PanikAttak said:
Thanks! I hope it’s still salvageable. I thought part of the issue was that I made the pseudo casing layer a bit too thick, because underneath that it all appeared to colonize evenly from the sides, but I probably didn’t mix it that well either since it was my first try at this. I think I really jumped the gun on this and tried to fruit it before it was ready.


We put our tubs in fruiting conditions immediately when spawning. Shoeboxes are always in fruiting conditions, even with the lid on.

Taking the lid off and putting it in what I assume is an improperly dialed monotub just ended up giving it more fresh air than it needed and conditions got wonky.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Way]
    #28349913 -

Hey guys,

Anyone know why my thicker sub is growing slower. I am doing GT and have 4 6qt unmodified shoeboxes. 2 of the shoeboxes have 1 quart of spawn with 1:1 ratio of coir and 2 shoeboxes have 2qts of spawn with the same coir ratio.

My 1qt shoeboxes are almost ready to harvest and the shoeboxes with 2qts have good size pins, but definitely taking longer. All of the shoeboxes look the same in terms of mycelium coverage and health. I do notice the 2qt shoeboxes have more moisture on the surface but they are just larger droplets.

I’m thinking it might be either humidity or FAE as there is more spawn/coir in the same size shoebox. It looks like they are still progressing as a good rate but seem like they are a few days behind.

Is this just a usual thing when you have a thicker sub layer?

Edited by Newshroom42 (06/06/23 07:44 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 2
    #28349937 -

You should have turned on voting immediately. Lol


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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW]
    #28349979 -

Asdfjkl;

When I inoculate grains I prefer to use ___

Ugh this poll better work now!
When I inoculate grains I prefer to use ___
Users may choose only one (31 total votes)
Agar
-
13 42%
Grain master
-
3 10%
Liquid inoculant
-
5 16%
Liquid culture
-
4 13%
Semi solid liquid culture
-
2 6%
Other, describe
-
1 3%
No answers, only results
-
3 10%
Votes accepted from 06/05/23 02:00 PM until 06/30/23 04:59 PM

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Re: Ask Quick Questions, Get Quick Answers [Re: Newshroom42] * 1
    #28350021 -

You may have mixed 2 qts that were inoculated with different cultures? If youre going to mix qts of spawn you should make sure theyre inoculated with the same plate of agar, or better yet g2g'd from the same jar.


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Edited by AspectOfTheCreator (06/06/23 09:36 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28350185 -

AspectOfTheCreator said:
You may have mixed 2 qts that were inoculated with different cultures? If youre going to mix qts of spawn you should make sure theyre inoculated with the same plate of agar, or better yet g2g'd from the same jar.


Ok that sound like it might be part of it. I used 2 agar cups that was from the same initial culture. I just checked one shoebox and it looks like It’s starting to take off and might need a dub tub it tomorrow now. I guess the little guys hard me and went into overdrive haha

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Re: Ask Quick Questions, Get Quick Answers [Re: Newshroom42] * 1
    #28350356 -

super quick question, when pouring LC (or agar) in a SAB, what do you do with the lid of the bottle? Just put it on piece of foil? Still sounds a bit sketchy since the foil will just be sitting there collecting whatever particles fell down.

I always finish my bottles so I've never given it much thought but this time I have to. I guess put it on a piece of foil and be extra careful when closing the bottle not to rub the lid on the edge of the bottle opening?:shrug:

Maybe I could put down a fresh alcohol wipe just before opening the lid and set it down on that.


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

Edited by altford78 (06/07/23 04:49 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Floret] * 4
    #28350357 -

Interesting that agar alone is so ahead. I like to use a grain master but do so less often than agar just because I tend to have more on agar and need to use it. As the options are either/or in the poll, it tends to skew to agar since there isn't any in between option. Thank you for posting it though. It is interesting to see the results so far.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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