TonyCanoli said:

Rizo? No? Too early to tell?  How do I know lol.

I feel like this looks really similar to my other transfers prior but it’s just more uniform.

Golden Teacher T3.

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mushhead said:

fiddle_head said:

mushhead said:

I'm having this issue with some plates too, showing wet looking growth that seems weak or bacterial even though the original culture seems clean as fuck.
I put some to grain the other day to see if they colonize and produce fruits without contam.
To fix the issue I am going to attempt a couple different agar styles.
My first is going to be: 8g Agar, 6g Malt Extract, and 1g Nutritional Yeast.
Second is the same but with activated charcoal, though I do need some advice on it's use as in how much, etc.
My third solution I'll use 150ml Distilled water with 350ml grain water, 8g Agar, and 1g Nutritional Yeast.
Hopefully one of these solutions wins out for me and I'll report back with my findings.

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I also hypothesize genetics and conditions; it’s a t2 isolation.
If I hadn’t had so many cups look like this I would say it were totally dependent on genetics but as these are clean transfers and I have had so many plates look like this recently, I’m wondering if my malt is aging and just need to replace it. A buddy of mine suggested PDA, which has been useful to me in the past; think I’ll give that a go.

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I purchased BULK Malt Extract, so much infact that the one purchase has lasted me about five years.
It could be because the Malt Extract is aging, though it has shown no change in color, smell, or consistency.
I get this growth from other methods of agar too, PDA, LMEA, and simple grain water+agar gives the same results.
I used to just toss pure grain water into 10g agar and it was perfect, but now for some reason it's not working as well for me.

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I've picked up some fresh Malt Extract, Fresh potatoes as well, and I'll be making grain water throughout tonight.
we'll see if I can stomp out that wet looking tepid growth.
I'll be making 20 plates of each recipe and recording my findings per plate very carefully.
Most likely I will put up a large post on my procedure and occurrences during this.

JSE said:

I wonder ig you guys can help me here please?

I took some grain from a colonized grain jar for agar before I put it to bulk (to avoid having to go from spores again).

These are the plates form the grain.

What do you think? How are they looking? Anything for grain, or particular areas to transplant to a new plate?

Thanks

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