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Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns]
    #28243646 -

I feel like my agar is too stiff for germinating spores. I use 10g agar (telephone brand) 10g lme to 500 ml tap. I’ve tried several different swabs and they take forever if they germinate at all. Is there a better lme recipe anyone can recommend to help with germination? Or is a softer water agar going to be better?

Thanks y’all

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision] * 1
    #28243648 -

Telephone is really hard, I've heard. Try 1.5% - 7.5g to 500 mL.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: dowodenum]
    #28243650 -

dowodenum said:
Telephone is really hard, I've heard. Try 1.5% - 7.5g to 500 mL.


Thanks man, I’ll definitely bring down the agar percentage.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision]
    #28243728 -

I just did 3 gram (if my scale is accurate) for 250 ml water and it came out how I like it and maybe a tiny bit soft but I think perfect.  Last time I use three I thought it was a little hard and that is why I mention the scale.  I am going to start just using about a 1/2 teaspoon with maybe just a pinch more and call it good for 250 ml. Haven't tried it yet but did see what 3 gram looks like in a teaspoon after weighing this last time.
Hope this helps more then hurts.
Cheers
gww

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JSE] * 3
    #28243739 -

JSE said:
Hello guys!

I am hoping you may be able to help me. I have been trying to progress from simple "home grow kits" (spore syringe into grain bag) to agar and home grain jars for best part of a year now, and have really been struggling with contam. I have slowly been upgrading my equipment, and now I think I am on the way.

I am having difficulty judging my agar plates - sometimes unsure what is contam and what is mycelium; and also having difficulty understanding how to judge healthy mycelium.

Would you guys be willing to talk me through the plates I have going at the moment? I have got rid of a dozen plates that were contaminated, leaving these ones:


I am most excited about this one. Should I do another transfer to agar? Or should I go to grain jars? Or possible to do both? If so, so you advise the leading edge of the thicker white mycelium?

Thank you all. I am really wanting to learn more about how to identify mycelium.


Stick with this culture because it is the most promising.

Pic 8 had some growth at the 5 o clock area which I would transfer from.

Everything else does not look promising and that last APE plate is contaminated like you suspected.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision]
    #28243810 -

MonoVision said:
I feel like my agar is too stiff for germinating spores. I use 10g agar (telephone brand) 10g lme to 500 ml tap. I’ve tried several different swabs and they take forever if they germinate at all. Is there a better lme recipe anyone can recommend to help with germination? Or is a softer water agar going to be better?

Thanks y’all


That’s exactly what I use to germinated with spore syringe. If I was doing a straight spore swipe, I would use softer agar. If you use that recipe, but soak the swab in sterile water first or made a spore syringe, it would be similar to what I do.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28243948 -

San Pedro Girl said:
MonoVision said:
I feel like my agar is too stiff for germinating spores. I use 10g agar (telephone brand) 10g lme to 500 ml tap. I’ve tried several different swabs and they take forever if they germinate at all. Is there a better lme recipe anyone can recommend to help with germination? Or is a softer water agar going to be better?

Thanks y’all


That’s exactly what I use to germinated with spore syringe. If I was doing a straight spore swipe, I would use softer agar. If you use that recipe, but soak the swab in sterile water first or made a spore syringe, it would be similar to what I do.


You use Telephone brand?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28244307 -

Thank you! OK so out of the 20-odd dishes I started with, I will just work from this one :grin: Can I ask what you would do with it, specifically?

-Take from only the thicker mycelium growth side? Disregard the other side or keep working with it?
-Take from the leading edge or anywhere?
-Transfer to agar again? Or transfer to grain? OR is it ok to do both - keep the genetics going on some agar plates and transfer some to grain?

Thank you

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JSE] * 1
    #28244329 -

How long after PCing agar plates do you let them sit to ensure there’s no contam before using?


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Here to learn

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Renoraines] * 3
    #28244341 -

If you let them sit too long, they might develop contams. I personally find that the sooner I use them, the better.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Renoraines]
    #28244345 -

That's an odd question, how are you pc'ing your plates? Wrapped in stacks or pp cups or similar?
You should pc them wrapped so that you can remove stacks to your sab or flowhood.
You don't need to wait to use them if they're cooled but if your concern is agar plates contaminating out of the pc then you have bigger concerns.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Renoraines]
    #28244369 -

Renoraines said:
How long after PCing agar plates do you let them sit to ensure there’s no contam before using?


As long as they weren't exposed to open air when moving from your PC to your sterile work space (hood/SAB) then as soon as they are room temperature

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Renoraines]
    #28244398 -

Renoraines said:
How long after PCing agar plates do you let them sit to ensure there’s no contam before using?


I do no-pour and hot pour(SAB). If I plan ahead, I proof them for about 4 days. But in practice I use them up about as fast as I can pour them. :tongue:


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This hobby has taught me many things.
Most of those things are patience.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28244399 -

Thank you.  Had read somewhere they let them sit for 5 days after.  I’m PCing in a presto 23q w 2oz pp5 ketchup style containers. Hopefully making my first batch tonight and am eager to get to trying.


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Here to learn

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Renoraines] * 2
    #28244403 -

Renoraines said:
How long after PCing agar plates do you let them sit to ensure there’s no contam before using?


I make as many at a time as I can and use them when I need them. That could be anywhere from as soon as they solidify to about a month. Rarely longer than a month. If sealed, your plates will last years.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: JSE] * 3
    #28244828 -

JSE said:
Thank you! OK so out of the 20-odd dishes I started with, I will just work from this one :grin: Can I ask what you would do with it, specifically?

-Take from only the thicker mycelium growth side? Disregard the other side or keep working with it?
-Take from the leading edge or anywhere?
-Transfer to agar again? Or transfer to grain? OR is it ok to do both - keep the genetics going on some agar plates and transfer some to grain?

Thank you


transfer from the rhizomorphic section at around 6 o clock. Take a few transfers to new agar and see what you can get cracking with that since its the best-looking myc u have so far. If those few transfers prove clean and rhizo id go big with them. Make as many transfers as you want from those plates and let them grow out 70% of the petri then send them to grain. I wouldn't go to grain yet with what you have.


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:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix] * 1
    #28245161 -

First time making/pouring agar, 15g lme, 15g agar, 750ml h20, I have slight condensation, started to pour around 129f, it’s slowly going away being in front of the flow hood. How long should I keep them sitting in front of the hood for before I proceed to wrap in parafilm?



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Extinction is forever




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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dirtnapz] * 1
    #28245240 -

I wouldnt trip and use as is.. You can leave em for a couple hours, but any longer they will start to dry out.. A little Condensation doesnt hurt anything.

Usually it will only be bad on top couple, to minimize stack back in 20’s once agar has solidified but is still warm and put the warm media bottle on top…

A hot cup of coffee/water placed on a plate will make most condensation go away..


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28247907 -

Hi all. I'm at my T4 of PE right now of my first try with agar, here's one plate: can you guys explain my why this transfer doesn't grow with ropey dense mycellium in all parts but just in some "branches"? All the other parts (in a circle) have a very subtle growth.



After the T0 and 4 transfers (takes time!) I'm willing to do my first cultivation from grain... is this good enough for you? I know you'd say to keep transfering, but for now I'd like to grow some fruits and see what happens.

EDIT: Here the Agar recipe I'm using:

4g potato flakes + 3,5g agar + 2g honey per 250 ml of water = 10 Petri dishes


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Edited by Nerex (03/26/23 04:16 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex]
    #28247917 -

If it's clean send it to grain mannn.

It looks like you're taking quite large transfers. If you take the size of a grain of rice as a transfer you can limit the variability in the rhizo/whispy growth. I personally would take the very leading edge of 4-5 o clock since it looks to be the fastest most rhizo growth and work with that. But all those rhizo sections look healthy to me.


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:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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