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Ababyphoenix
You

Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 9 days, 16 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushhead]
#27133199 - 01/07/21 03:12 PM (5 years, 8 months ago)

mushhead said:

You gave up too soon looks like.

I know right... Its all good ill have many many more clones to toy around with soon. Btw I modded those tubs and the shoeboxes are looking much better. thanks for rushing me :heart:

:heart:

Im not a pro culture er but I think shes a bit past due for a clone at this point?

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:sporedrop:

:aum:

Pho's MushiAventures: :aum:

:aum:

:sporedrop:

:heartpump:

:heartpump:

:peace:

:peace:

Edited by Ababyphoenix (01/07/21 03:13 PM)

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starbones
I'm an alien, I eat uranium.

Registered: 03/04/20
Posts: 1,131
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ababyphoenix]
#27133206 - 01/07/21 03:16 PM (5 years, 8 months ago)

Aight so

10g agar
10g ELME
1g nute yeast
500ml tap water.

Just for clone plates I want them to stretch and grow out nice and quick.

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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: starbones]
#27133370 - 01/07/21 04:29 PM (5 years, 8 months ago)

I’d reduce the lme then if you want to be lower nute. Maybe 6g lme and the 1g nutritional yeast or leave the yeast out all together.

My standard everyday use recipe for 500ml is like

10g agar
7g lme

I don’t personally use yeast as it makes my myc go haywire but others use it with great successes

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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tomcards

Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 3 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27133417 - 01/07/21 04:51 PM (5 years, 8 months ago)

D3,

In your agar tek, you recommend 10g agar and 8g lme for 500ml. That's what I've been using.

Have you changed your recommendation?

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tomcards]
#27133430 - 01/07/21 04:56 PM (5 years, 8 months ago)

That’s fine too, doesn’t make a huge difference. I’m always experimenting back and forth trying to find what performs best. Id say anything between 6.5-8g lme is a pretty solid everyday use range.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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starbones
I'm an alien, I eat uranium.

Registered: 03/04/20
Posts: 1,131
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27133527 - 01/07/21 05:43 PM (5 years, 8 months ago)

When you say haywire what do you mean? Is it harder to find sectoring?

--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: starbones]
#27133540 - 01/07/21 05:47 PM (5 years, 8 months ago)

I went from perfect rhizo myc to something that looked like a white fluffy paintball splatter I only gave it one chance and my results are subjective

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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starbones
I'm an alien, I eat uranium.

Registered: 03/04/20
Posts: 1,131
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27133571 - 01/07/21 05:59 PM (5 years, 8 months ago)

Alright I'll try some test plates using it with some clone tissue and some without then decide, thanks man.

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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.

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GrinchGrower
N00B

Registered: 10/02/20
Posts: 682
Last seen: 5 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: starbones]
#27133607 - 01/07/21 06:16 PM (5 years, 8 months ago)

I'm just gonna take all these translucent plates to grain.
I think I saw someone having a similar issue said it bounced back after transferring to new medium.

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:minifo:

:minifo:

Trusted Cultivator Methods & Mushroom Cultivation Info :minifo:

:minifo:

:snowman:

:snowman:

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AlsetAlokin
Student

Registered: 07/30/20
Posts: 182
Last seen: 5 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: GrinchGrower]

1
#27133632 - 01/07/21 06:26 PM (5 years, 8 months ago)

Myc growth in 3 hours.

PE t2 from spores

Higher res non-magic picture.

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Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: bigfootscreepyuncl]
#27133682 - 01/07/21 06:42 PM (5 years, 8 months ago)

I've been using 9g Agar powder. Seems plenty soft to me. The spores didn't complain. 8g made the agar a little too sticky for my liking. Stuck the scalpel too far into the piece and it didn't wanna let go for the transfer.

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NOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushhead]
#27133806 - 01/07/21 07:16 PM (5 years, 8 months ago)

TheDoobsker said:

What I've been doing for condensation is right after pouring plates, I boil about 300ml of water and add it to my media bottle, put the cap on the bottle and place it on the top of the stack. All the plates are crystal clear.

This isn't anything new, I think a lot of growers do something similar. I have just found that using the media bottles works really well

mushhead said:

Set your vessel on a stable unmoving surface while it cools from the PC(hopefully you're using a flowhood or SAB), let the bubbles disperse and allow it to cool to 125f or so, pour.
Some agar needs to be shaken when it comes out of the PC, if need be grab heat resistant gloves and shake it up way before the 125f mark, let the bubbles disperse obviously.

So as for bubbles🤓, when i go to use agar I’m reheating it in the ‘wave. Some bubbling inevitably occurs. I guess i should have said that. By the time I cool it down it’s still there.

As for condensation I have also heated the media bottle (250ml) and used on one plate at a time. Beer bottle works too but next time i look at them they are condensed up again. Im hoping to learn how to avoid it all together. I tried cooling down a little more last time but no luck. 44 degrees is about as far as I can take it before it clumps up. Still condensation. Maybe my room is too cold?

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Edited by NOS4A2 (01/07/21 07:22 PM)

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Mr. Mushie

Registered: 01/16/20
Posts: 667

Re: AGAR ENVY! (Anything and All things agar!)

[Re: GrinchGrower]

1
#27133816 - 01/07/21 07:19 PM (5 years, 8 months ago)

GrinchGrower said:

I'm just gonna take all these translucent plates to grain.
I think I saw someone having a similar issue said it bounced back after transferring to new medium.

https://www.shroomery.org/forums/showflat.php/Number/27088878

Not sure if you've seen this, but this might help you.

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GrinchGrower
N00B

Registered: 10/02/20
Posts: 682
Last seen: 5 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mr. Mushie]
#27133840 - 01/07/21 07:27 PM (5 years, 8 months ago)

Mr. Mushie said:

GrinchGrower said:

I'm just gonna take all these translucent plates to grain.
I think I saw someone having a similar issue said it bounced back after transferring to new medium.

https://www.shroomery.org/forums/showflat.php/Number/27088878

Not sure if you've seen this, but this might help you.

Thanks for the redirect!
It looks like he had some luck with higher nutritional content.
Will report back if I get any good results going to grain or new plates.

--------------------

:minifo:

:minifo:

Trusted Cultivator Methods & Mushroom Cultivation Info :minifo:

:minifo:

:snowman:

:snowman:

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The Dalcassian
Dirty Druggie

Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: GrinchGrower]
#27134301 - 01/08/21 12:12 AM (5 years, 8 months ago)

I had a similar issue, dropped this plate to grain and it colonised perfectly. Took a grain from it back to my plate and the same growth reoccurred by T1.
Culture was fine tho

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Here Lies This Individual

Edited by The Dalcassian (01/08/21 12:13 AM)

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starbones
I'm an alien, I eat uranium.

Registered: 03/04/20
Posts: 1,131
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Dalcassian]
#27134676 - 01/08/21 08:05 AM (5 years, 8 months ago)

How long is it usually taking you guys to see clone growth from tissue on agar? On average.

--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.

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roarkell
The Roar Master!

Registered: 08/29/19
Posts: 2,223
Loc: Southern WI

Last seen: 2 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: starbones]
#27134688 - 01/08/21 08:13 AM (5 years, 8 months ago)

2 or 3 days to see jump off... but my temps are a little low... 68-72ish...

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starbones
I'm an alien, I eat uranium.

Registered: 03/04/20
Posts: 1,131
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: roarkell]
#27134710 - 01/08/21 08:28 AM (5 years, 8 months ago)

From inner tissue of a fruiting body?
Weird. I always get a week from a mature fruits inner flesh. On MEA agar, that's why I'm trying lower nute stuff in the hopes to speed that up. I know RR says the myc has to revert but a week seems excessive.

Thanks man

--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.

Extras:

roarkell
The Roar Master!

Registered: 08/29/19
Posts: 2,223
Loc: Southern WI

Last seen: 2 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: starbones]
#27134791 - 01/08/21 09:24 AM (5 years, 8 months ago)

A week seems like a really long time... im usually checking the plates like 24 hours later... lol. Inner tissue and plate pins are usually in that 2-3 day range for me.. . My agar is 2% agar 1.5% LME...

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Ababyphoenix
You

Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 9 days, 16 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: roarkell]
#27134804 - 01/08/21 09:39 AM (5 years, 8 months ago)

:whathesaid:

--------------------

:sporedrop:

:aum:

Pho's MushiAventures: :aum:

:aum:

:sporedrop:

:heartpump:

:heartpump:

:peace:

:peace:

Extras:

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Shop:

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