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jcm4620
Stranger

Registered: 05/26/19
Posts: 6,700
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Re: AGAR ENVY! (Anything and All things agar!)

2
#27102014 - 12/22/20 05:12 AM (5 years, 9 months ago)

i always do that wben taking germination points off a streak plate. i dont wanna waste plates for no reason. im nit one of these ppl that spends 6 months on agar b4 running anything. i dont ever let them grow out much bigger than that. i knkw exactly what i want to take and where i want to take it from. especially at t1. im always on grain by t2 but in the worst case scenario t3 but almost always t2. however those plates il let grow out to about the size of a qtr with 3 on a plate. then they go right to grain👍👍👍

my goal is clones and i want to start fruiting as fast as possible. when ur sittin there making a million transfers at some point u could be transfering away from something you may want without even knowin it. i want my initial gene pool as big as possible. plus wasting time on letting them grow out that big when each 1 goes to its own grain jar is just kinda pointless.

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PANAEOLUS FRUITING MADE SIMPLE

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27102020 - 12/22/20 05:24 AM (5 years, 9 months ago)

:whathesaid:

Very cool dude! Gonna be the new way for meeeeeeeee!

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jcm4620
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Registered: 05/26/19
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]

1
#27102024 - 12/22/20 05:28 AM (5 years, 9 months ago)

yup aka already done😃 took them this mornin b4 work they should b on grain within 2 or 3 days i imagine

i very rarely will ever let them get mich bigger than this

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PANAEOLUS FRUITING MADE SIMPLE

Edited by jcm4620 (12/22/20 05:51 AM)

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27102055 - 12/22/20 06:13 AM (5 years, 9 months ago)

jcm4620 said:

yup aka already done😃 took them this mornin b4 work they should b on grain within 2 or 3 days i imagine

i very rarely will ever let them get mich bigger than this

Ahh so tiny, so cute! Not sure I could see that well in the SAB.
But hey, are those 60mm plates? Sorry, curious minds and all...

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jcm4620
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Registered: 05/26/19
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27102167 - 12/22/20 08:34 AM (5 years, 9 months ago)

no they r 100

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jcm4620
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Registered: 05/26/19
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27102169 - 12/22/20 08:35 AM (5 years, 9 months ago)

also no need to see em that good cuz i just cut around em and toss in a jar🤷🏼

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PANAEOLUS FRUITING MADE SIMPLE

Edited by jcm4620 (12/22/20 08:35 AM)

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Eugene Gesuale
Jar-Sniffer

Registered: 04/12/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27102522 - 12/22/20 01:03 PM (5 years, 9 months ago)

Anybody know if macaroni cook water would work like grain soak water for plates?

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Narrator
Strangest in the night

Registered: 11/30/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Eugene Gesuale]
#27102545 - 12/22/20 01:26 PM (5 years, 9 months ago)

Mold or myc? Day 18 since starting from spore syringe. 250 ml water/5g BRS/5g agar.

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]

1
#27102553 - 12/22/20 01:30 PM (5 years, 9 months ago)

jcm4620 said:

also no need to see em that good cuz i just cut around em and toss in a jar🤷🏼

Ok thanks for your time!
This is really helpful...

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GETTING STARTED :sporedrop:

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Hide
I Have No Bung Hole

Registered: 11/25/14
Posts: 159
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27102607 - 12/22/20 02:13 PM (5 years, 9 months ago)

First clone attempt. Contam in bottom right. Are the dots all over contam too? Would an 8 o clock transfer be wise?

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bigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Hide]
#27102693 - 12/22/20 03:45 PM (5 years, 9 months ago)

Here's an update on my first attempt at agar (MS syringe -> agar -> t1)

Here's the mother dish, inoculated on 12/9

t1 12/16(double transfer, obviously)

t1 12/16

t1 12/16

t1 12/16

I was hoping to get two of these transferred to grain by New Years and it looks like I'm right on track, if not a little ahead of schedule. My plan is to use 2 plates to inoculate 5-6 jars each in order to start clone hunting and continuing to transfer the other plates just to see what happens.

I have a syringe of GT, PE and APE eagerly waiting for plates of their own with the same strategy in mind. I'm going to need to get some more tubs soon. Cheers, mates!

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I

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dingdonger22
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Registered: 12/14/20
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: capntrips7]
#27102926 - 12/22/20 06:33 PM (5 years, 9 months ago)

light box pictures looking great!

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Hide]
#27102956 - 12/22/20 06:57 PM (5 years, 9 months ago)

Hide said:

First clone attempt. Contam in bottom right. Are the dots all over contam too? Would an 8 o clock transfer be wise?

It looks as if you moved the clone tissue all over the place. Those dots look like cube myc to me, but they're similar in size and quantity to the little mold satellites that you get all over the place when you fuck up royally from technique.

Anyway, if it happens to be mold you're going to find out really soon. At any rate I would do a transfer right now.

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Ras_Irie
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Registered: 07/30/15
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]
#27103280 - 12/22/20 11:00 PM (5 years, 9 months ago)

Advice needed! Want to make PDA agar have no karo syrup, cant buy it fast but need to do transfere from multi spore agar.I did potatoe, honey, carbon agar. Get germination of spores, but I am conserne abiut honey is it ok to use it? Growing cubes mycelium.

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mushhead
Livonia Wanderer

Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ras_Irie]
#27103337 - 12/23/20 12:17 AM (5 years, 9 months ago)

Boil potatoes in water for about 30min(small batch)-1hr(big batches like too much PDA *wink wink*).
This extracts from them the starches and nutes you want.
Filter as best as you can.
Grab 500ml of it and add 10g agar.
PC as you would any regular agar vessel. I PC for 45min but that's because I'm crazy.
Now you have PDA. Enjoy.
I don't see a small amount of Honey being a bad addition to your agar as I have used it minimally in Liquid Culture.
PDA is pretty straight forward.

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TigerStrike
carpophore locator

Registered: 12/22/20
Posts: 315
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushhead]
#27103419 - 12/23/20 02:15 AM (5 years, 9 months ago)

mushhead said:

Boil potatoes in water for about 30min(small batch)-1hr(big batches like too much PDA *wink wink*).
This extracts from them the starches and nutes you want.
Filter as best as you can.
Grab 500ml of it and add 10g agar.
PC as you would any regular agar vessel. I PC for 45min but that's because I'm crazy.
Now you have PDA. Enjoy.
I don't see a small amount of Honey being a bad addition to your agar as I have used it minimally in Liquid Culture.
PDA is pretty straight forward.

Some recipes recommencement peeling the potatoes for better clarity in the finale product what do you think?

https://www.shroomery.org/forums/showflat.php/Number/27103145
This is the LAGM2021 I need an agar that will not let me down and make me look bad at my first agar attempt that people can learn from!!! Should I buy Antibiotics since I am going wild spore print to agar? or just be clean and transfer a lot?

Save on Gas or electric or babysitting time the pc unless its tradition or you enjoy the Quality time with the Pc. Think about Just run your agar for 30 min. Some run theirs for 15 min!!

I have brewers yeast as an additive what think you what do you think?

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jcm4620
Stranger

Registered: 05/26/19
Posts: 6,700
Last seen: 4 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27103458 - 12/23/20 03:14 AM (5 years, 9 months ago)

Inthepit said:

jcm4620 said:

also no need to see em that good cuz i just cut around em and toss in a jar🤷🏼

Ok thanks for your time!
This is really helpful...

thats only for pans by the way tho

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27103552 - 12/23/20 05:14 AM (5 years, 9 months ago)

:homerdoh3:

"thats only for pans by the way tho "

I was thinking (uh oh) I'd do that with APE.
Guess I missed the part where the PANs are different in that respect.

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:sporedrop:

GETTING STARTED :sporedrop:

:sporedrop:

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GrinchGrower
N00B

Registered: 10/02/20
Posts: 682
Last seen: 5 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27103560 - 12/23/20 05:33 AM (5 years, 9 months ago)

Took transfers from these tonight...looking a little better.
Forgot to snap a close up of the GT plate.

I went through and tossed out ~25-30 old plates I had been hoarding.
It seems less is more in this hobby, and wasting all that plastic sucks.
:feelsbadman:

:feelsbadman:

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Trusted Cultivator Methods & Mushroom Cultivation Info :minifo:

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TigerStrike
carpophore locator

Registered: 12/22/20
Posts: 315
Last seen: 4 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: GrinchGrower]
#27103570 - 12/23/20 05:48 AM (5 years, 9 months ago)

I feel like you would waste time additional expense sterilizing washing re sterilizing glass dishes too though. Sucks.

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Shop:

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Injection Grain Bag

Liquid Cultures

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Unfolding Nature: Being in the Implicate Order

Spores for European Microscopy

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