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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26979051 -

Any idea what's up with this plate? It's been pretty damn slow and it decided to sector out kinda weird.


This plate was transferred the same day.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26979057 -

Tha blank spots may be from contam.

The rings around that first culture indicate high temp swings, the cold nights are probably what's slowing it down


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26979058 -

Yes I worry that I'm rushing it, especially taking half
a plate. So there's discipline, doing transfers till an actual
clean plate is achieved. And how does one know, well that comes
from experience!


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26979060 -

My cultures have been growing at inconsistent speeds too, I think the ones on top of the stacks are slower, but I haven't played with it to be sure.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #26979136 -



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish]
    #26979144 -

LoadedFish said:
What’s the highest amount of grain jars you guys have nocced up from a single pasty plate? Wondering if I could use one culture to inoc 7 jars


Sometimes I split a single 100mm plate into 12 jars. You could probably get away with 6-8 jars from a single pasty plate. Not the best way to do things tho. Just throwing it out there.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26979145 -

tryptkaloids said:
Tha blank spots may be from contam.

The rings around that first culture indicate high temp swings, the cold nights are probably what's slowing it down


Thats probably accurate. The temp is usually around 68Β°F in the morning and 72Β°F through out the day.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #26979209 -

I may wait, then. The only reason I asked is because I finally have a culture I’m confident in, but my other culture seems to have contammed which ruined my plan to do 2 plates into 7 jars. I was worried about the clean culture hitting the edge by the time I can get another plate ready so I guess I’ll have to transfer from that too.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish] * 1
    #26979253 -

LoadedFish said:
I may wait, then. The only reason I asked is because I finally have a culture I’m confident in, but my other culture seems to have contammed which ruined my plan to do 2 plates into 7 jars. I was worried about the clean culture hitting the edge by the time I can get another plate ready so I guess I’ll have to transfer from that too.


Don't get too caught up in it like I did. I spent weeks making transfers trying to get that perfect plate when I really could have dropped a wedge and been over the hump. For whatever reason, I was under the impression that I needed a full plate. Plates kept contaminating before full growth and just kept transferring. Then this happened.

Doctor Mario said:
I confess that that tonight I was stoked to advance from agar to grain. I had a nice puck that I went to put in a grain jar and it fell off the scalpel and landed face down on a wet towel. πŸ€¦β€β™‚οΈ So disappointing... I put in the jar anyway but I guarantee there is no way that its not gonna contam. Oh well. I should have another nice puck ready in about a week and a half.


LotKid said:
I use a single piece about the same size as if i were transferring to another plate.

The trick is not waiting too long to start shaking. To give you an idea... if the piece of agar is the size of a dime... when the growth looks like the size of a quarter i'm shaking if i havent done so already. My first shake is usually maybe 3 days after knocking up the jar.

Once myc grabs onto a nice lil cluster of grains around the agar... shake. Then like 3 days later... shake it again. Your jars should finish faster.


Utilizing that advice, I dropped some wedges about nickel-quarter size and waited until they looked like this.



I gave 'em a good shake and rolled the jars around to get the colonized oats to distribute a little better and when the myc grabbed onto another cluster of oats, they got shook and rolled again.



Once the colonized oats looked pretty well distributed through out the jar,

I left them alone for a week and came back to this.



Some of the wedges that went into these jars came from plates that had mold and bacteria growing in them. Obviously you want to stay well away from that part of the plate as to not accidentally touch it with the blade and work real slow and careful or your jar will end up like this.


Don't forget, if you have 1 good jar, you potentially have many more of the same culture if you G2G.


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Edited by Doctor Mario (10/10/20 11:13 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26979268 -

Quote:

LotKid said:
I use a single piece about the same size as if i were transferring to another plate.



Idk abt you but thats like a 1/4 inch for me


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26979276 -

This made me feel a lot better about the situation lol. I hadn’t even considered putting the clean parts of the contammed plate to grain. I’m probably gonna keep an eye on it and see if it looks safe, and if not I’ll just inoc everything with the clean one. As a first time grower my fear of spawning to bulk and finding trich or some shit in the tub is overwhelming

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish] * 1
    #26979284 -

tryptkaloids said:
Quote:

LotKid said:
I use a single piece about the same size as if i were transferring to another plate.



Idk abt you but thats like a 1/4 inch for me</font>


Yea, same here. I didn't have the balls to do one that small. I was worried that perhaps I didnt hydrate the grain enough and it would dry out before full colonization.
LoadedFish said:
This made me feel a lot better about the situation lol. I hadn’t even considered putting the clean parts of the contammed plate to grain. I’m probably gonna keep an eye on it and see if it looks safe, and if not I’ll just inoc everything with the clean one. As a first time grower my fear of spawning to bulk and finding trich or some shit in the tub is overwhelming


This is my first time as well and I understand that fear just the same. Just make sure that you've got some transfers on stand by so if something goes south, you dont end up back to germinating spores.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #26979290 -

I hope it's ok for me to post this here, I'm an utter noob and I just completed my first transfers from MSS Pastyplates. I'm hoping to get some feedback on whether or not I made correct choices and where I could have done better for future reference.

These were all inoculated on 9/28 and transfers took place today (10/10). I know I should have made the T1 transfers from MSS plates at the earliest sign of mycelium, but it is what it is at this point. It was so tomentose I wasn't sure what to transfer or whether it was mycelium or mold (plus I was just nervous I was going to screw it up). I used a pre-mixed MEA that I bought on Amazon plus some red food coloring, and I'm wondering if the nutrient level of the pre-mix is why my mycelium was so cottony. The Golden Teacher mycelium was probably at least 8mm thick!

I also noticed while reviewing this post that it looks like I took these pictures with my hand and phone hovering over the open plate - I did not. I'm dumb, but I'm not quite that dumb. The plates were inside my SAB, my phone was outside of it. There was a sheet of Lexan between them. Obviously, you cannot take a decent picture of Pastyplate mycelium with the lid on they way you can with proper petris. I just wanted to mention that before someone called me out for holding my hand over the open plates. I did try to avoid letting my hand ever cross over the plates.

-------------------------------------------------

So up first was Golden Teacher. I had one plate that just never kicked off. It didn't contam, it just did nothing. The other plate was a little slow to kick off, but then turned into what seemed like a very consistent growth pattern. Here is the before and after so you can see what I transferred:

 

I know it looks like I took the cuts with a rusty machete while blindfolded, but I swear it was a flame-sterilized scalpel with a new blade. I just suck at using it so far. I had a hard time choosing what to transfer, so I just took wedges of what seemed like the most aggressive myc.

-------------------------------------------------

Next up was Mazatapec. I had one contam on me so I tossed it (contam pics at end for advice) and one made it. As you can see, I had a little control issue with the syringe here, and let several drops go:

 

Again, I just took what seemed like the most aggressive mycelium for my two transfers.

-------------------------------------------------

Lastly, we have Penis Envy. One of these was actually the first plates to show growth, and it remained the most aggressive. It was also the only of the four varieties I inoculated two plates each with in which neither plate contaminated and both produced mycelium. The second plate, while it produced nice looking mycelium, wasn't nearly as aggressive as the first so I wound up leaving it untouched and instead took three transfers from the first plate. The first one (yeah, I got a little spastic with the syringe here, too):

 

And here is the one I didn't transfer from:



-------------------------------------------------

I also inoculated two plates with PF Classic, but one contaminated and the other never showed signs of any life. I inoculated two more plates from the MSS tonight, fingers crossed - otherwise, I'm going to start worrying about the syringe and the 6 PF Tek jars I also nocced with it.

-------------------------------------------------

I'm going to include pics of the two I tossed for contamination, just for confirmation. This first one I'm sure was bad, the contamination showed up in under 48 hours. Any thoughts on what it was?:



The next one showed up around 72 hours, and at first I thought it could be mycelium, but ultimately decided it was too soon and too close to the edge, and must be mold, so I tossed it. Do you think I was right, or did I toss mycelium?:



-------------------------------------------------

So, in total, I had two contaminate and two show no growth out of 8 plates. Not a great start, but at least I got something for my efforts.

I can only assume that I didn't dribble enough out of the syringe into my waste bowl after flame sterilizing the needle and before inoculating the plates for the two that showed no signs of life. I was inoculating 6 PF Tek jars per variety, flaming the needle every two jars, then flaming it again before noccing the plates. I must have nocced two of them with BBQ'd spores.

As for the contamination, I'm sure my (not very) sterile method sucks. It will hopefully get better. I may also have contaminated them flailing the plates around in my SAB trying to shake the condensation out of them. I'll know from now on to make up my Pastyplates at least 5 days ahead of needing them (and don't put them in the fridge!) so the condensation can evaporate a little.

Again, I hope this isn't the wrong place to post this, but since it seems like some of the board regulars frequent this thread, I was hoping to get some experienced input on whether I should have made different decisions on where I took my transfers. Any and all input/advice/criticism is welcome, wanted and appreciated!


[Edit to add: I have kept all of the original plates I transferred from, so if you do see something that you think I really missed the boat on, please let me know. I can still transfer it! Assuming I didn't contaminate the plates, of course.]

Edited by Forgotten Oak (10/11/20 12:01 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26979291 -

Best of luck and may we both see bountiful harvests:gethigh:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish]
    #26979456 -

What am I growing here fellas? Can I get a pro's take? Anything ready for grain of should I keep cleaning it up?

AA Plate 1



AA Plate 2



AA Plate 3



Malabar Coast Plate 1



MC Plate 2



MC Plate 3



MC Plate 4



--------------------
Mehhh



Edited by Wall.E (10/11/20 06:21 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26979473 -

Not a pro but they definitely all look like cube myc to me. Are those satellites on the third plate between 1-5 o’clock or just reflections from the agar?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yeetusdeetus]
    #26979495 -

Yeetusdeetus said:
Not a pro but they definitely all look like cube myc to me. Are those satellites on the third plate between 1-5 o’clock or just reflections from the agar?


Just reflections lol


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26979516 -

Dr mario, if you have any kind of contamination on your plate I’d transfer away from it to agar and only use clean plates to knock up jars.


--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26979549 -

D3monic said:
Dr mario, if you have any kind of contamination on your plate I’d transfer away from it to agar and only use clean plates to knock up jars.


Yeah, I agree. I check all my plates daily and try to transfer at the first indication of a colony beginning to form. If the myc growth on the plate is large, I just quarter it up staying away from the contam and put it to grain. If its only big enough for 1 jar, I get 3 or 4 small transfers to new plates.

I've gotta figure something out. My contam rate is out of control all of a sudden and I'm not exactly sure why. I lost 16/20 plates to trich and a white mold. Its extremely agressive with very long strands. Ive changed the air filters in the house, scrubbed and cleaned everything real good and try to be real careful in the SAB. Mold is heavy in this area and I think its getting brought inside with us. My kids and dogs are constantly in and out of the house.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26979566 -

Quote:
I lost 16/20 plates to trich and a white mold.


Is that fresh plates or plates with a wedge?

From what I've read it's only in the SAB that matters. This island
is one big petri dish, even the politics! :rofl:

So I analyzed only my SAB related practices.
I'm down from 50% to 2.5% (inside joke).

Soooo...without further ado...

Washed 90 Qrt SAB, rack, and table, w/Clorox & towel
Books on top of SAB to anchor it down and reduce effects of bumping.
Nitrile gloves, mask, glasses, hat, iso gloves, (forearms soaped).
Wet towel under 3” high rack.
Lysol wipes under plates
iso the scalpel handle before
Hold scalpel toward the end
try flaming the entire blade all the way down to where it locks on.
Move slowly, don’t disturb the air
Lift petri dish cover very slowly
Move stack completely away from being over the bottom plate so fingertips aren't over the open plate.
don't bring the media bottle all the way back when pouring.
Don't bother cooling the blade, be the sizzle!
work at the back of the SAB
Evil spirits residing in the room?
New Plates in shoeboxes now, we'll see
Try some incense to see air movement

Oh and D3
When wrapping plates with grafting tape, can you just break the tape
or is it so strong you have to scissor it?


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (10/11/20 08:01 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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