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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor]
    #26677604 -

We'll see how it goes, I plan to keep the plate nonetheless if it remains clean even after taking cuts.
It Def is going apeshit


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead]
    #26677608 -

That is some thick myc.

What is it like a week after putting it on the plate?


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26677610 -

Four days actually. They were test plates to make sure that my LC was clean.
In fact the plates have grown faster than my jars from the same LC.


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Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26677730 -

A.k.a said:
Since there’s no scientific evidence supporting either one why would you doubt the anecdotal evidence?

I’ve never heard anybody say myc only grows on top of agar but that’s definitely not true.

To me it made sense that there would be multiple genetics based on how mushrooms work, then this grow was enough to convince me for sure. Same clone grown side by side, only one is an early plate and one was transferred a few times.



Mainly because this 'hobby' has moved in leaps and bounds over the last 10 years where science has changed how we approach growing and the outcomes reflect this.

When different species of active mushrooms fruit can't be separated without a microscope, and we want to make assumptions on the visible presentation of it's myc?

I guess some folks are happy hiding in their bliss :shrug:

As for folks saying myc only grows on the surface of agar. . . Most agar teks in here mention 2D growth to spot contamination. . . I assumed 2D meant. . . Well 2 Dimensions :shrug:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #26677931 -

Bobbit said:
A.k.a said:
Since there’s no scientific evidence supporting either one why would you doubt the anecdotal evidence?

I’ve never heard anybody say myc only grows on top of agar but that’s definitely not true.

To me it made sense that there would be multiple genetics based on how mushrooms work, then this grow was enough to convince me for sure. Same clone grown side by side, only one is an early plate and one was transferred a few times.



Mainly because this 'hobby' has moved in leaps and bounds over the last 10 years where science has changed how we approach growing and the outcomes reflect this.

When different species of active mushrooms fruit can't be separated without a microscope, and we want to make assumptions on the visible presentation of it's myc?

I guess some folks are happy hiding in their bliss :shrug:

As for folks saying myc only grows on the surface of agar. . . Most agar teks in here mention 2D growth to spot contamination. . . I assumed 2D meant. . . Well 2 Dimensions :shrug:


Funny, I was just fascinated by this a month ago... I did samples of the underlying agar to prove its colonization...



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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26677952 -

Did you cut down the middle with a scalpel?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RyeHumor] * 1
    #26677968 -

RyeHumor said:
I have a bunch of contaminated plates that I hope I was able to grab something of today not sure though. Might just have a ton garbage plates from these. we shall see :shrug:



I like the way that the contam'd GT and B+ plates grew.

Here are a couple of the plates I'm excited for.


And my first invitro pin!



Tried my 100mm Poly Pro dishes as no pours, unfortunately I didn't let them cool in the PC properly so they had way more condensation than I would have liked.

probably going to order one of those 500 packs from celltreat, those are my favorite by far.

Have a few more MS plates that I am hopeful for, but it seems like the myc looks way different than I am used to. Either that or its all contamination. Time will tell.


Damn RH you putting in work bro. There seems to be plenty of usable myc there. You'll get it. Just gotta get your sterile Tek down make sure nothing ever passes over a open plate besides the scalpel when dropping a wedge.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ModularMind]
    #26678018 -

ModularMind said:
Did you cut down the middle with a scalpel?


Good question. Assuming you are suggesting the initial cut exposes the surface to myc from the blade. . . Plausible, though I have transfers that grow fluff on the bottom, not exposed to the blade, and A2G where myc grows out the bottom to grain. Also noticed that when i cut the outside cut first (or A2G where the whole thing goes in), 5mm from the leading edge of myc, the remaining face can start growing, often from the bottom (petri) edge.

Still more rigourous testing required. . . Like flipping half a plate (folding down the middle from one edge) and using a fresh scalpel to take transfers from the underside, without touching the colonised side?

I used a modified innoc. Loop to 'punch' round samples. . . Those that land myc down, still grow fluff on the underside. . .

I have a few 25mm thick 0.5% agar pucks in wide mouth 1/2 pints. . . One has P.sub nearly colonised to the edge. . . I reckon the colonisation is not along the surface only. . . It will be interesting to see how quickly it takes to grain. . . If only I had used the same agar to make some 3mm thick as a comparison. . . Would be a bitch to get out of the petri though as it is too soft to extract in one piece. . .

Might try next round with a sterilised teaspoon for the transfer. . . If the jars with more agar, and same growth size on surface colonise considerably quicker, would that suggest the myc has colonised as a sphere? I'm hoping so, the reason for making real soft agar. . . Poormans blenderless LI. . .


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The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #26678081 -

mushhead said:
Wouldn't it stand to reason that by taking a clone of a singular fruit you are already isolating?
I am by far no expert on the subject, just stating an observation that I've seen with my GT clones growing uniform rhizomorphic hyphae without having to narrow it down from MS.


You're narrowing down, but not isolating.

Bobbit said:
As for folks saying myc only grows on the surface of agar. . . Most agar teks in here mention 2D growth to spot contamination. . . I assumed 2D meant. . . Well 2 Dimensions :shrug:


It's still mostly 2D. What you're saying is that a drawing isn't truly 2D because colour has some thickness to it. Also, in a properly made dish, nothing will germinate below the surface.

Bobbit said:
Still more rigourous testing required. . . Like flipping half a plate (folding down the middle from one edge) and using a fresh scalpel to take transfers from the underside, without touching the colonised side?



I don't think there's a lot of testing needed. Put your wedge to grain and watch the back side of the wedge getting fluffy in a day.


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How I do glass dishes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ModularMind] * 1
    #26678230 -

I did slice with a scalpel. I think its pretty accepted that mycelia colonizes the agar throughout.... Transfers grow out on the inner exposed agar. Food coloring gets consumed... This test of mine.

I have found that the internal agar colonization is effected by the nutrient content of the agar. If the nutes are low... say around .7% The inner agar gets colonized at about the same speed as the surface.

If the nutes are higher... say, around 1.6%, the agar gets colonized at about half the speed of the surface.

I have been studying this because I have had varying results with liquid inoculant and I wanted to hunt down why it sometimes was awesome. Other times not so much.

Current feelings: very low nutrient agar makes for fast growing, but weak mycelia that doesn't handle a blender well.

Dream LI agar plate: 1) Deep pour (lots of stuff in LI syringe) 2) soft agar (sub-1% agar so it blends easily) 3) Higher nutrients (2% or more nutrients so mycelia is strong for the LI)

Unknown: If my LI is improved by having some agar uncolonized when blended, or if its better to wait til the entire puck is colonized throughout before blending.

Currently, I am colonizing 4 quart bags of winter wheat berries at 5 days every time with a half petri dish. I doubt I can get it much better.... but If I wait for the whole dish to colonize, it may get even faster. That would be about 3 days past when the mycelia reaches the wall of the dish. (when the nutes are at 2%)


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26678294 -

I think four quarts in five days is as good as it’ll get. Maybe you could shave a few hours off but at this point I’d bet you’ve got the tek dialed in and the culture makes the most difference.

I gotta get a blender. This two week colonization stuff has isn’t doing it for me.


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LAGM2020

Edited by A.k.a (05/18/20 08:48 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26678307 -

"Dream LI agar plate: 1) Deep pour (lots of stuff in LI syringe) 2) soft agar (sub-1% agar so it blends easily) 3) Higher nutrients (2% or more nutrients so mycelia is strong for the LI)"

Tedoro that sounds good, I'm gonna give it whirl. :hatsoff:
5g Agar = 1%, 10g LME = 2%    500mL
Oh and mebbe 50% Oat broth...

  (  last agar was; 10g Agar 2%, 7.5g LME 1.5%, 500mL and 100% Oat broth!  )
    Oh ya and Team Telephone since yer agar may vary bud!  LOL


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (05/18/20 09:16 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26678330 -

I have a blender but the results are still not quite there. I think the low nute agar I'm using is the main issue. From my experience, myc recovers way faster if its fed properly. I'll be making some new plates today so I went with 1.5% LME and I hope it's going to work better.


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How I do glass dishes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
    #26678338 -

Poisoned what were you using before?
2% Agar, 1.5% LME is what c10 calls standard...

"Same as any cube, I'd start with my standard half strength mea (20g agar, 15g LME, 1L water). If that doesn't work I'd try soft agar (11g agar, 15g LME , 1L water)- c10  so that's 10gA, 7.5gLME, 500mL and 100% Oat broth! 1.5% nutrient + broth, 50 min PC - c10 2% Agar, 1.5% LME "


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (05/18/20 09:11 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26678342 -

My last batch was 0.7% nutes and 1% agar. My agar seems to be stronger than telephone brand because at 1% it's still pretty very solid and won't really break up. I'm also planning to let it soak in water before blending it next time to make it absorb as much water as possible.


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How I do glass dishes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
    #26678350 -

"to let it soak in water before blending it next time to make it absorb as much water as possible"

Interesting, but it sounds like there would be more condensation.
I tried SAGARI but the agar wedges were like little greased pigs! And a lot of condensation!
(  3.5, .7% to 5g, 1% of agar, and 6.5g 1.3% of powdered LME to 500ml of water  )


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (05/18/20 09:29 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26678360 -

What I'm saying is to take a sterilized blender attachment jar, put the plate inside then wait a few hours before blending and nocing up. Or give it just one pulse on a blender, wait out for a few hours and then blend it up.


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How I do glass dishes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26678363 -

My struggle has been having high nute, low agar dishes can make for funny looking growth... I make sure my source mycelia is perfect going into these LI recipe dishes... but still, it can get fuzzy or dive under the surface... kinda messing with the whole purpose of LI... allowing confirmation of no contams right up to the point of grain inoculation.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
    #26678369 -

poisoned said:
What I'm saying is to take a sterilized blender attachment jar, put the plate inside then wait a few hours before blending and nocing up. Or give it just one pulse on a blender, wait out for a few hours and then blend it up.


I've done this, but I have found that my agar plates, at room temp, seem uninterested in taking up more water and softening.

I have been brainstorming if there is any other method of breaking down the gelatinous aspect of the puck, while leaving the mycelia unharmed. Perhaps a chemical breakdown, instead of mechanical.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (05/18/20 09:36 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26678384 -

While we are on the topic, and since I've been having some breakthroughs... I thought I'd mention that my focus has been to colonize the agar internally, blend it just small enough to get into my syringe (1mm or so) and mix it immediately with the grains, a lot. Because I am counting on the mycelia emerging from these small pieces of agar... I have no interest in waiting a day before mixing, as is common advice. I'm seeing leapoff 24hrs later.

I spent years doing LI with 2.5 week colonization times. Lots of problems arose from that.

Interested in doing deep dish petri plates with a 10mm puck. That might spread one plate over 8 bags or more with these same 5 day times I'm getting. Pretty nice. I'll keep you posted.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (05/18/20 09:44 AM)

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