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poisoned
untitled



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Poisoned's glass petri no-pour tek. 6
#26680235 - 05/19/20 03:29 AM (3 years, 8 months ago) |
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I see a lot of people on here wanting to use glass dishes, but there's not many teks on them. While glass dishes aren't for everyone, I believe everyone should have at least a couple of them at home as a backup.
You don't need boro dishes to do it. I've been using soda lime glass dishes for quite a long time now and none have cracked in a PC. There's a common misconception that you need boro for any high temperature application, but it actually doesn't matter in situations where you get nice even heating. Your jars are made of soda lime glass and if they're sitting in water during your PC cycle, they will experience way bigger temperature differences than your agar dishes.
Also, many people are cautious about boiloff. I had it happen a few times when some dishes were filled all the way to the brim. Try to keep your agar level at about half height for best results.
So, let's get to work. You'll need:
- PC
- Some glass dishes
- Agar
- Saran wrap
- Vinegar
First, make your agar. Use whatever recipe you want. I like to put agar and LME into cold water and let it sit for at least an hour before cooking it. It seems to bring out zero sediment and perfect clarity. Then put it on a stove and cook it until everything is dissolved nicely. Be careful, because it likes to boil over and it's a bitch to clean. Pour it into a pitcher or a bottle or whatever you want to pour with:

Lay out your dishes on the counter like this:

Pour them:

If you're as clumsy as I am, you might have poured some over the edges. Nothing to worry about, you'll just have some extra work.
Let agar cool in the dishes. Once it's solid enough, use a paper towel and clean up any agar that didn't end up in dishes. Make sure to clean edges and bottoms thoroughly.
Now take out your saran wrap and stack dishes onto it like this:

Wrap them:

An important thing to note here is that you want saran on the bottom to be flat, so your dishes will sit level in your PC. You also want only one layer of saran. You'll see why later.
Put your dishes in a PC. I use some espresso cups to lift the rack above the water, use whatever you have that will keep your rack level:

Make sure there's a tiny bit of space between individual stacks. Otherwise, they'll all stick together. If you don't have enough room to do that, but some tin foil between the stacks.
I do a 20min cycle with it. You can cycle it however long makes you feel comfortable. Let the PC cool down completely before moving it or anything. You want your agar to solidify before you take your dishes out, otherwise you'll spill over all your hard work.
Here come the dishes:

They will be a bit moist, but wrapped relatively tightly. There will be some holes in the saran wrap and that's a good thing. Just leave them on some shelf and they will dry out in a day or two. You can also go ahead and use them, but then I prefer to leave them unwrapped in a SAB for a day to let them dry out. I usually do that anyways.
Ok, so you grew some myc and some contams on those dishes and need to reuse them. I dump agar pucks into the toilet, but I'm not sure if that's recommended. My toilet sewage pipe basically goes straight down into the septic tank, so I can get away with lots of stuff.
Then I put them in a sink with some water and do some scrubbing:

I don't scrub too much. Just enough to remove the labels and any other visible debris. I put dishes into a sweaterbox sized tub, with edges pointing upwards. Once I'm done with all of them, I put some vinegar into the tub and cover all dishes with water:

This step is a must unless you get some super soft water out of your tap. It will keep your dishes nice and clear over time. Let it soak for a few hours.
Then put your dishes into a strainer, with edges pointing down:

Let it dry for a day and you have your dishes ready to be poured again.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26680259 - 05/19/20 03:59 AM (3 years, 8 months ago) |
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Nice! I didn't know cling wrap would hold up in a PC cycle, I would've thought to use foil.
If I wanted I could just sterilize the plates and pour them after they cool, yeah?
--------------------
 Amateur Mycologists United AMU Q&A
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poisoned
untitled



Registered: 04/17/13
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Re: Poisoned's glass petri no-pour tek. [Re: mushpunx]
#26680279 - 05/19/20 04:18 AM (3 years, 8 months ago) |
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Yeah, for sure.
Cling foil does melt, but in a weird way, where it just pulls together.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26680443 - 05/19/20 06:12 AM (3 years, 8 months ago) |
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Makes sense. I might get a set of these, it would be nice to have them to put spores on, clone tissue, etc.. plates I'm gonna toss right away anyways.
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 Amateur Mycologists United AMU Q&A
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FrankRhizo
Interdimensional Travel Agent

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Re: Poisoned's glass petri no-pour tek. [Re: mushpunx]
#26680452 - 05/19/20 06:19 AM (3 years, 8 months ago) |
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I like it. Thanks.
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StinkBorg
Stranger

Registered: 04/11/20
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Re: Poisoned's glass petri no-pour tek. [Re: FrankRhizo]
#26680488 - 05/19/20 06:55 AM (3 years, 8 months ago) |
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Very nice. I really appreciate the write up. You cleared up a lot of good questions I had surrounding the topic. I think you may have motivated me to go finally try working with glass dishes.
I also didnt know plastic cling wrap would hold up at all through the PC. Super cool!
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The Fresh Prints
Smell ya later



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Posts: 1,377
Loc: Bel-Air
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Re: Poisoned's glass petri no-pour tek. [Re: StinkBorg]
#26680504 - 05/19/20 07:13 AM (3 years, 8 months ago) |
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Hell yes. I was just getting ready to buy a bunch more of the holy grail no-pour containers. I think I'll give this a try instead.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,654
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Good to see a write-up on glass. I've just started experimenting with recycling my agar. Beginning with spore plates that didn't germinate. I just re-wrap them, re-pc them, reuse them. No problems, big time saver. One day soon will try with contaminated plates.
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poisoned
untitled



Registered: 04/17/13
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Re: Poisoned's glass petri no-pour tek. [Re: LadysKnight]
#26680681 - 05/19/20 09:15 AM (3 years, 8 months ago) |
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It should work, but then you end up with some visible specks in your grow.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,654
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26680784 - 05/19/20 10:09 AM (3 years, 8 months ago) |
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Probably right, we shall see. Did I mention I'm a cheap, lazy bastard? Anyway, nice needed Tek, good job.
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Adas
Lonely Dreamer



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Re: Poisoned's glass petri no-pour tek. [Re: LadysKnight] 1
#26683302 - 05/20/20 12:52 PM (3 years, 8 months ago) |
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Same here, I had no idea SW could survive PCing. The lifting of the rack is a genius idea, idk why I haven't thought of it before. I sterilize my dishes in a mycobag, which sucks for the most part, lol. I think I'll try your method next (or slightly modified).
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poisoned
untitled



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Re: Poisoned's glass petri no-pour tek. [Re: Adas] 1
#26683568 - 05/20/20 03:11 PM (3 years, 8 months ago) |
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As said, SW doesn't survive it like you wrapped it. It will have some holes, it will melt together, but I find it even better that way.
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ouuwee
Garden of Weeden


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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26683930 - 05/20/20 06:05 PM (3 years, 8 months ago) |
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Where do you get your glass petris
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poisoned
untitled



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Re: Poisoned's glass petri no-pour tek. [Re: ouuwee]
#26684683 - 05/21/20 01:06 AM (3 years, 8 months ago) |
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I'm located in central Europe and I get them for 0,75€/piece from a local lab store.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,269
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26684748 - 05/21/20 02:36 AM (3 years, 8 months ago) |
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I got mine from a German vendor but despite being pretty good, they weren't as specified. Not an honest vendor (they were smaller then they should have been).
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Bobbit
Even Stranger

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Re: Poisoned's glass petri no-pour tek. [Re: Adas]
#26696098 - 05/26/20 02:09 AM (3 years, 7 months ago) |
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Nice writeup.
Will try raising the rack to allow more water for a slower cool. . .
Like the vinegar also. . . I've been drying properly till shiny, which is a lot of work. . .
For those looking to recycle plates, make sure you remove any visible colonies first. . .
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Inthepit
Aum Mani Padme Hum



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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26701426 - 05/28/20 10:06 AM (3 years, 7 months ago) |
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Well I'll try it again. I think you encouraged me in another thread. And even though I can't stand the smell of vinegar, I definitely want my new plates to stay pristine! I totally dig my borosilicate glass and just use PP for fridge storage.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Poisoned's glass petri no-pour tek. [Re: Inthepit]
#26701499 - 05/28/20 10:41 AM (3 years, 7 months ago) |
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Cool writeup nice work
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poisoned
untitled



Registered: 04/17/13
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Re: Poisoned's glass petri no-pour tek. [Re: Pastywhyte]
#26701766 - 05/28/20 12:42 PM (3 years, 7 months ago) |
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Thanks man! Means a lot to hear that from Pastywhyte himself!
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Poisoned's glass petri no-pour tek. [Re: poisoned]
#26704804 - 05/29/20 02:23 PM (3 years, 7 months ago) |
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Really appreciated! I bought a 12 pack of pyrex when I first started and then decided to go with celltreat instead. I kind of hate wasting the plastic, though. I have a set of 40 Alien holy grails, too. They are great for general use. But I still felt bad about the box of unused pyrex. Now I am inspired to at least use these for clone masters for the best clarity.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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