Poisoned's glass petri no-pour tek.

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poisoned
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Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 3 years, 10 months

Poisoned's glass petri no-pour tek.

10
#26680235 - 05/19/20 03:29 AM (6 years, 3 months ago)

I see a lot of people on here wanting to use glass dishes, but there's not many teks on them. While glass dishes aren't for everyone, I believe everyone should have at least a couple of them at home as a backup.

You don't need boro dishes to do it. I've been using soda lime glass dishes for quite a long time now and none have cracked in a PC. There's a common misconception that you need boro for any high temperature application, but it actually doesn't matter in situations where you get nice even heating. Your jars are made of soda lime glass and if they're sitting in water during your PC cycle, they will experience way bigger temperature differences than your agar dishes.

Also, many people are cautious about boiloff. I had it happen a few times when some dishes were filled all the way to the brim. Try to keep your agar level at about half height for best results.

So, let's get to work. You'll need:

PC
Some glass dishes
Agar
Saran wrap
Vinegar

First, make your agar. Use whatever recipe you want. I like to put agar and LME into cold water and let it sit for at least an hour before cooking it. It seems to bring out zero sediment and perfect clarity. Then put it on a stove and cook it until everything is dissolved nicely. Be careful, because it likes to boil over and it's a bitch to clean. Pour it into a pitcher or a bottle or whatever you want to pour with:

Lay out your dishes on the counter like this:

Pour them:

If you're as clumsy as I am, you might have poured some over the edges. Nothing to worry about, you'll just have some extra work.

Let agar cool in the dishes. Once it's solid enough, use a paper towel and clean up any agar that didn't end up in dishes. Make sure to clean edges and bottoms thoroughly.

Now take out your saran wrap and stack dishes onto it like this:

Wrap them:

An important thing to note here is that you want saran on the bottom to be flat, so your dishes will sit level in your PC. You also want only one layer of saran. You'll see why later.

Put your dishes in a PC. I use some espresso cups to lift the rack above the water, use whatever you have that will keep your rack level:

Make sure there's a tiny bit of space between individual stacks. Otherwise, they'll all stick together. If you don't have enough room to do that, but some tin foil between the stacks.

I do a 20min cycle with it. You can cycle it however long makes you feel comfortable. Let the PC cool down completely before moving it or anything. You want your agar to solidify before you take your dishes out, otherwise you'll spill over all your hard work.

Here come the dishes:

They will be a bit moist, but wrapped relatively tightly. There will be some holes in the saran wrap and that's a good thing. Just leave them on some shelf and they will dry out in a day or two. You can also go ahead and use them, but then I prefer to leave them unwrapped in a SAB for a day to let them dry out. I usually do that anyways.

Ok, so you grew some myc and some contams on those dishes and need to reuse them. I dump agar pucks into the toilet, but I'm not sure if that's recommended. My toilet sewage pipe basically goes straight down into the septic tank, so I can get away with lots of stuff.

Then I put them in a sink with some water and do some scrubbing:

I don't scrub too much. Just enough to remove the labels and any other visible debris. I put dishes into a sweaterbox sized tub, with edges pointing upwards. Once I'm done with all of them, I put some vinegar into the tub and cover all dishes with water:

This step is a must unless you get some super soft water out of your tap. It will keep your dishes nice and clear over time. Let it soak for a few hours.

Then put your dishes into a strainer, with edges pointing down:

Let it dry for a day and you have your dishes ready to be poured again.

--------------------
How I do glass dishes

Extras:

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