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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26523601 -

c10h12n2o said:
D3monic said:
Well this is a new one for me. Going a little geometric. Didn’t have the lines when I took transfers. Just some fluffy run away edges acting weird on malt yeast agar





That's weird af. Myc always does weird shit when I use yeast, so i stick to plain mea these days

woofwoof said:
Is it safe to use sterile swab to make transfers or is cutting it with scalpel to make transfers cleaner or better?

I have some agar with myc I want to clean by moving to a new plate. Can I swab the myc and streak new plate?



Itll never be clear because brf doesnt dissolve completely like ME does. If you want clear get some LME and boil and  pour it through a cheescloth before PC, like I do in the guide in my sig. Crystal clear every time

Also why 30psi ? Why not 15psi for 20 min, the standard?

You could technically use anything sterile.. but scalpel is best for precision,  scalability, reuse, etc.. I love #7 handles



Yea I think I’m going to stick to only using it for streak plates. All my xfers were looking really good and as soon as they hit the yeast they got funky looking so this last transfer I went back to mea. Just wanted to give Mistys agar mixes a try.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 2
    #26523705 -

D3monic said:
Well this is a new one for me. Going a little geometric. Didn’t have the lines when I took transfers. Just some fluffy run away edges acting weird on malt yeast agar





Illuminati.


--------------------
Trade List
🖕🖕🖕
6 hole Mini Monos

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Re: AGAR ENVY! (Anything and All things agar!) [Re: gizmo1]
    #26523751 -

gizmo1 said:
D3monic said:
Well this is a new one for me. Going a little geometric. Didn’t have the lines when I took transfers. Just some fluffy run away edges acting weird on malt yeast agar





Illuminati.




PASTY! EXPLAIN THIS SHIT! I knew the RW was special, but this thing's trying to talk to us through symbols....

Edited by Sockadin (03/08/20 11:12 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sockadin]
    #26524442 -

Does this look like mold to anyone else?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #26524445 -

Looks like my melmak frist few xfers

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #26524481 -

p9hu7 said:
Does this look like mold to anyone else?






Mold would of overtaken the dish by now.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #26524497 -

Good point.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #26524508 -

Doesnt look clean tho. But I doubt its all mold

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #26524510 -

mushboy said:
p9hu7 said:
Does this look like mold to anyone else?






Mold would of overtaken the dish by now.



What kind of mold? I'm finding most of my contam mold does not spread out as far as myc... my mold circles seem to stay much smaller

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #26524513 -

mushboy said:
Doesnt look clean tho. But I doubt its all mold



Dirty af, just T1 away from spore.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: C12ShroomMan]
    #26524535 -

How do my 2 plates look? i thought they were good so i took some transfers


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Re: AGAR ENVY! (Anything and All things agar!) [Re: C12ShroomMan]
    #26524567 -

I just got my first mold that looks like myc today. Was on a spore plate and though it was myc germinating. Took a transfer and the next morning it’s the size of a 50 cent piece.

Anybody ever seen this red contam before?  It’s on a melmak swab plate from Jan 1.  I think certain bacteria eat the food coloring and concentrate it cuz I’ve seen some pretty flashy colored contams over the last couple months since I got the neon food color.


Plate was originally dyed light pink.

Dirty ass swab plate with “myc”


--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26524612 -

So I have these t2 transfer plates, and I am throwing one away due to contamination, and I was able to get some good surface pics. I am wondering why My second transfers are growing so damn slowly. Is it bacteria? If so how can i Get rid of it? My recipe for this agar is 500 ml water 15g agar 20g oat boil broth and a drop of honey. This transfer was done 2 weeks ago.



I know Its contaminated, but its a good example for how most of the transfers look. Very small and thin myc. I really need help figuring out how to get these to give me good healthy mushrooms in the future.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26524731 -

Thanks hitler! Being patient is the hardest part.  Will report back when I see some progress.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: landscraper]
    #26524763 -

huangdeez said:
So I have these t2 transfer plates, and I am throwing one away due to contamination, and I was able to get some good surface pics. I am wondering why My second transfers are growing so damn slowly. Is it bacteria? If so how can i Get rid of it? My recipe for this agar is 500 ml water 15g agar 20g oat boil broth and a drop of honey. This transfer was done 2 weeks ago.



I know Its contaminated, but its a good example for how most of the transfers look. Very small and thin myc. I really need help figuring out how to get these to give me good healthy mushrooms in the future.



For one you are using way too much agar. I use 20g agar per 1000ml for hard agar , and sometimes that's too hard. Use 10g for 500ml. I also dont like using simple sugars. Grain soak water is fine by itself, but I prefer good ole MEA (20g agar, 15g LME, 1L water) any day

Looks like you have at least 2 kinds of contams there, that dark one is already sporulating

Cant really see that well what the light ring around the myc looks like. But I would start over if it was me


landscraper said:
Thanks hitler! Being patient is the hardest part.  Will report back when I see some progress.



Yeah definitely:) you should have something to work with soon


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26525630 -

When I transfer a wedge does it matter what side of the wedge I put on the new plate ? For example do I flip the wedge so the myc is on the bottom of the new plate ? I ask this because I have fuzzy growth on the top of the wedge then they grow out more flat .

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye] * 1
    #26525636 -

It doesn’t matter, it will eventually find its new food source and colonise the plate.


--------------------
Back once again with the ill behaviour



Links I like

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #26525729 -

I found some of these in with some contaminated dish. Is this mold or myc? It grew 10 days to that size.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: huangdeez]
    #26526017 -

Hard to tell. I’ve had myc grow kinda like that on soft plates where it was under the surface, but def doesn’t look like normal growth.


--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26526172 -

Looks like Mold to me but I could be wrong. Give it a few more days see how it grows out.


--------------------
Back once again with the ill behaviour



Links I like

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