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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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First time grower, first time agar-er
    #25951016 -

Hi, new to cultivation and agar. I've studied so many teks at this point my head is spinning. I plan on updating this thread as things progress. Here's where I am so far.

I prepared a SAB, gathered alcohol, gloves, metal inoculating loop and a  torch. I started by inoculating 3 plates via loop. 3 days after that inoculation I inoculated two other plates, but I scraped the print a little firmer this time. I was concerned I didn't use enough pressure on the loop to the print, as I was keeping in mind the common advice on not scraping too hard. Tactile sensation on the inoculation loop is fucky.:grin:

14 days after inoc, the original 3 plates still show no signs of, well, anything. Is 3 weeks a good 'shit or get off the pot' timeframe?

11 days later after inoc, the second two plates show the following.
 


Forgive me if the images lack resolution, my phone camera is outdated and I do not own a HD camera.

The two dots in question appear to be white in color, high density in the center and lower at the edges. I do not see anything in the dishes of another color or shape. I do wonder if it's too early to tell.

Edit: The prints were GT.

Edited by ManicMonk (04/23/19 02:50 PM)

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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25951030 -

Yeah from the pics they look like myc let them grow out to the size of say a quarter then transfer


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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25951038 -

Interested to hear what replies here. I'm also new to the agar. I've got a few plates innoced with an MS syringe that look similar. There are loads of what I'd describe as blotchy white dots. White and furry/fuzzy in the middle and lighter at outside.

I'd say we've both got some kinda contam it's just wether it's salvageable. Is it possible or worth it to cut a piece from middle of dot and transfer?

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Re: First time grower, first time agar-er [Re: thisbliss]
    #25951055 -

That's not a contam.. and you always transfer from the leading edge .. the furry fuzzy is called tomentose mycelium rhizomorphic growth happens after s few transfers and also by lowering the nutes


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Re: First time grower, first time agar-er [Re: Boogieman47]
    #25951092 -

Ah ok cheers hopefully all's good then! Would u advise to transfer now or wait for more growth?

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Re: First time grower, first time agar-er [Re: thisbliss]
    #25951100 -

I say once it gets to the size of an american quarter .. maybe an inch in diameter


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Re: First time grower, first time agar-er [Re: Boogieman47]
    #25951115 -

Hey Thanks Boogie. After they reach that inch diameter, how large should slices be? I'm thinking around 20-25% of the total.

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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25951123 -

We shoot for a grain of rice or smaller ..


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Re: First time grower, first time agar-er [Re: Boogieman47]
    #25951146 -

Right on, thank you sir.

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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25951224 -

No worries good luck


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Re: First time grower, first time agar-er [Re: Boogieman47]
    #25952572 -

Checking the plates today, one of the plates has grown very quickly.



The disconcerting bit may be not visible in the photo. The center of the growth appears to be a dull green/grey color. Green can't be good, surely.

The other plate remains white throughout.

Edited by ManicMonk (04/24/19 10:32 AM)

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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25952579 -

That don't look good, especially since it's on the edge of the plate.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: First time grower, first time agar-er [Re: ShaperDreaming]
    #25952687 -

Does anyone know what that leading edge is. It's not white but it isn't exactly same as the agar so something is already growing on it. Or is it just what happens before the mycelium begins to form on the agar?

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Re: First time grower, first time agar-er [Re: thisbliss]
    #25952701 -

I can only offer that it appears the leading edge is a translucent white,appears to grow at the same rate as the denser center.

It looked like myc before the green showed in the center, afaik.

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Re: First time grower, first time agar-er [Re: ManicMonk] * 1
    #25952743 -

ManicMonk said:
I can only offer that it appears the leading edge is a translucent white,appears to grow at the same rate as the denser center.

It looked like myc before the green showed in the center, afaik.


I believe that the "clear" or "transparent" leading edge is just dikaryotic mycelium growth.

That's still mycelium... it's just not cube mycelium.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: First time grower, first time agar-er [Re: ShaperDreaming]
    #25952759 -

Cheers don't mean to hijack. Had seen it on mine too. So when you're advised to cut from the leading edge to transfer do you include this 'clear translucent' myc or stick with white rhizo?

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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25952773 -

ManicMonk said:
Checking the plates today, one of the plates has grown very quickly.



The disconcerting bit may be not visible in the photo. The center of the growth appears to be a dull green/grey color. Green can't be good, surely.

The other plate remains white throughout.


Yeah that doesnt look good to me either now thay it has grown out a bit I'd say it's a contam  what does the other one look like ???


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Re: First time grower, first time agar-er [Re: thisbliss]
    #25952775 -

thisbliss said:
Cheers don't mean to hijack. Had seen it on mine too. So when you're advised to cut from the leading edge to transfer do you include this 'clear translucent' myc or stick with white rhizo?


So, I don't know as much of the science as others, but I can explain why you transfer from the leading edge, which will hopefully answer your question.

When you transfer from the leading edge you're grabbing mycelium that is both A) Actively growing, and B) Not hiding contam. When you grab from the leading edge you want to cut about a grain of rice size because you want to ensure that you're taking as little of the medium needed to spark more growth.

How much of which type to grab is going to be more about your technique and experience than anything. When you grab from the thicker parts of mycelium there is a small chances that it is hiding something underneath, or within it. It's agar so it's easier to see, but without a lot of experience it is still challenging to tell exactly what you're transferring, and even after a half of a year at this I still have a hard time diagnosing mycelium on agar.

Honestly, without a microscope it's all a little bit of guess work when it comes to mycology.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

Edited by ShaperDreaming (04/24/19 12:40 PM)

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Re: First time grower, first time agar-er [Re: ManicMonk]
    #25952778 -

That looks like aspergillus niger although full disclaimer it would be very hard to tell without microscopy or time lapse photos. Aspergillus niger is slow growing, starts white and transitions to tan (or sometimes green) before turning black. When it turns black it sporulates. One colony can easily create a half dozen more in a week. Colonies are small, half the size of a dime or so. It is commonly found in soil contained in indoor potted plants. Some strains produce mycotoxins that can be harmful to humans and pets. I have this mold in my home and as long as I handle my prepours correctly in my semi sterilized SAB it is not an issue.

IF YOU FOLLOW THE ADVICE IN THE NEXT PARAGRAPH DO IT WELL AWAY FROM YOUR LAB! PREFERABLY OUTSIDE OR IN YOUR GARAGE!!

If you must save that plate I would make a makeshift GB from a cardboard box and glad wrap with shopping bag glove holes. Put your donor and receiving plate, alcohol, scalpel, lighter and rag in the box. Flame sterilize, transfer, seal and thoroughly wipe then toss the box and donor plate. I would transfer at least once more afterward before cloning just to be sure. AN is not a competitor once the Mycellium takes hold but can wreck your grain jars in days. Adding 10% peroxide solution to your receiving dish should be sufficient to clean your culture.

Here is a picture of an air quality drop plate I took 3 weeks ago. It begun as two tiny white spots... DO NOT OPEN THAT INSIDE!


Edited by Professor X (04/24/19 12:49 PM)

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Re: First time grower, first time agar-er [Re: Professor X]
    #25952790 -

Here is a link for diagnosing contaminants (scroll down to "how it shouldn't look", but the first part is good too).

Here's another, slightly better thread that is singularly focused on spotting contaminants.

Professor X said:
IF YOU FOLLOW THE ADVICE IN THE NEXT PARAGRAPH DO IT WELL AWAY FROM YOUR LAB! PREFERABLY OUTSIDE OR IN YOUR GARAGE!!

If you must save that plate I would make a makeshift GB from a cardboard box and glad wrap with shopping bag glove holes. Put your donor and receiving plate, alcohol, scalpel, lighter and rag in the box. Flame sterilize, transfer, seal and thoroughly wipe then toss the box and donor plate. I would transfer at least once more afterward before cloning just to be sure. AN is not a competitor once the Mycellium takes hold but can wreck your grain jars in days. Adding 10% peroxide solution to your receiving dish should be sufficient to clean your culture.


It looks like mold to me, which type I wouldn't know without growing it out, and I don't recommend growing it out.

Also that technique... I don't recommend that. Where did you hear this tek?

Just use a regular SAB with good sterile technique and transfer as far away from that contam as possible. If you're feeling like it's risky don't have any other work happening in the box. If you're getting this mold on your plate then your house is filled with the spores anyways.


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