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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Agar Tips
    #25842592 -

I just finally recently got into agar after always having contaminants when I inoculated with spores. So now I'm hoping to finally get a successful grow with no contaminants using agar. I wanted to show some of my petri dishes and see what you guys think and if there's any tips you would give me on how to best work with agar.


These pictures show the second transfer I did of a petri dish I had inoculated with spores.
I took the best looking rhizomorphic growth and transferred it to new dish.



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Re: Agar Tips [Re: TrippnBB]
    #25842609 -

Weeeel, looks ok maybe. 

Try to not double dribble like that.  One culture per plate. 

Looks ok.  Try to take smaller wedges, they transfer easier and balance on the blade easier.


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Need a lift?

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Re: Agar Tips [Re: BeefExtreme]
    #25842622 -

The best tip is never have your hand or finger directly over open media.


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Agar Tips [Re: JHOVA]
    #25842635 -

JHOVA said:
The best tip is never have your hand or finger directly over open media.


and when using a SAB, hold the plate at roughly a 45 degree angle so the top of the plate isn't perpendicular with the roof of your SAB


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Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrÑn and it means that of which is of itself.

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Re: Agar Tips [Re: Ziran]
    #25842670 -

Definitely take better pictures of your plates. When bacteria is often hidden, you don't want shadows from your camera obstructing the growth. You can angle plate in front of a light and snap clear pictures. Or if they're laid flat like you've done, make sure they're lit up better. Here's some examples:







Your Maz plate has solid growth on the right, but clear bacteria from 6-7 and 10 o'clock. I'd definitely transfer on the right growth only. The PE growth in the second plate looks clean, but a better picture might reveal more. I'd definitely take a handful of transfers from that plate. The final PE plate has clean growth, but obvious satellite colonies to the right of it. I'd take a few transfers from the left side of the clean growth.

You should record and upload your next SAB session for critique. There's a million threads with overall technique advice, including videos of SAB sessions. Here's a thread where I'm  requesting critique of my technique when learning agar. I advise others to do the same if they're looking for tips on lowering their contam rate.

An actual recording of your session can immediately tell us what needs to be fixed. Kind of a waste of time to give basic pointers that have been covered ad nauseam when you can just upload a video and we can see exactly what you're doing wrong. I expect everyone just learning or struggling w/ agar to have already learned the basics from searching.


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ATTACK OF THE KILLER APE!!!11 | G2G Video | Stare Sticks | Bags in SAB |
DMT + Laser = Reveals Alien Code Commonly Reported In Trips | JavaScript I Use To Make Shroomery Better [New]

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Re: Agar Tips [Re: stareatclouds]
    #25842673 -

Oh, and for a beginner, your plates look very good. Your clean growth looks awesome, so keep it up, dude.

My one tip would be to realize that not every contamination will be a separate, distinct green colony or growth of a different color or texture. Those are easy to spot (especially bacteria because it smells horrible), but molds intertwined with mycelium are not (this is where holding up to a light can help). Fortunately I don't seem to get many contams these days, but unfortunately, this has rendered my ability to differentiate between clean mycelium and tiny bits of mold fairly poor. Search for "spotting hidden contams in agar" or something similar and there's a dope thread on this.

There's a slight learning curve to agar, but once you get it down, your mycological possibilities are endless. Props to you for ditching spore syringe inoculation (that's for dumbasses). I definitely had my share of satellite colonies and bacteria when first learning, but have a very low contam rate these days. As you keep practicing, your muscle memory takes over and you'll make effortless transfers with no fear. Once you know enough to make the purposeful, direct movements, you stop fucking around with open plates, crossing over your hands, and knocking shit around in your SAB. And then your contam rate goes way, way down.


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ATTACK OF THE KILLER APE!!!11 | G2G Video | Stare Sticks | Bags in SAB |
DMT + Laser = Reveals Alien Code Commonly Reported In Trips | JavaScript I Use To Make Shroomery Better [New]

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Re: Agar Tips [Re: stareatclouds] * 1
    #25842878 -

I don’t open my blank plate until I have the wedge on the blade.  Even then, I don’t set the lid down with my left hand, but hold it at an angle, opening the plate just enough to get the wedge transfered, then I close it. 

Everything that comes to the work table gets sanitized with an alcohol wipe.  Plates and anything else that goes in the sab gets wiped down again before entering. 

Blade is wiped and burned after each plate.  Gloves and even wrists and forearms are sanitized after each plate. 

A spray bottle with alcohol is nice. 

As said before this isn’t bad work for your first time.  Just breath steady and focus on smooth and concise movements.  Don’t do it stoned.  Don’t do it hungry, particularly if your hands shake from hypoglycemia like mine do.

Edit, also label your plates with the transfer number.  T1 means first transfer from a germ plate. 

I use  the code ct1 for first transfers from a clone plate.  Ct2, ct3 etc.
It’s important.  Let’s say you spawn a t1 plate and take a clone.  Let’s say the clone doesn’t work out well in the tub.  It won’t do to go back to a ct plate, you need to go back a generation to t1 to try again. 

It’s simple for you at this point but it starts getting more complex with each generation.  I keep t1 and t2 plates for this reason. 



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Need a lift?

Edited by BeefExtreme (02/28/19 08:15 AM)

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Re: Agar Tips [Re: BeefExtreme]
    #25843075 -

Something that lowered my contams a lot was keeping my x-actos in cup of alcohol (outside SAB).  No need to wipe it with iso towel.  I saw it on one of Bod's videos and tried it out.  A lot better than setting the scalpel down somewhere that isn't clean.


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:sporedrop:Unmodded, Unflipped, No Drill, Monotub TEK:sporedrop:

:stoned:OTD - Let's Talk Music:stoned:

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Re: Agar Tips [Re: rickyswamps]
    #25843467 -

rickyswamps said:
Something that lowered my contams a lot was keeping my x-actos in cup of alcohol (outside SAB).  No need to wipe it with iso towel.  I saw it on one of Bod's videos and tried it out.  A lot better than setting the scalpel down somewhere that isn't clean.



Aye,  I saw that too.  I had a little jar of alcohol I was doing that with, but the sexy move would be to have one of those things barbers keep their blades in.

I think it’s calles a barbacide.


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Need a lift?

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Re: Agar Tips [Re: rickyswamps]
    #25843873 -

rickyswamps said:
Something that lowered my contams a lot was keeping my x-actos in cup of alcohol (outside SAB).


Do you mean for storage in between SAB sessions? Not during, right? I assume you're not taking it out of an alcohol cup and then flame sterilizing.

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Re: Agar Tips [Re: badsponge]
    #25843883 -

Yes actually


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Need a lift?

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Re: Agar Tips [Re: BeefExtreme]
    #25843911 -

Doing great man, except for the bacteria in areas. I didn't read all the posts but you are aware of the obvious bacteria, right? I'd take another transfer (smaller) of each. I think you'll be ready for grain then (assuming you don't fuck up your sterile tek). Stareatclouds is spot on with his advice. Great post, stare. If you've got specific questions feel free to ask. Otherwise we'd just have to throw out all ten million standard tips and hope we hit something you are curious about.

BeefSupremeJr said:

Try to not double dribble like that.  One culture per plate. 




Maybe good advice for a beginner just because the more times you put a transfer in the more times you risk contaminating the plate. But if you are good enough I highly advise "double dribbling". Actually i find the tripple dribble to be the best use of the available space. I only go to one dish per plate if I'm actively cleaning a filthy culture or if I'm going to use the plate for grain. Unless and until then, I triple dribble like a mofo.



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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Agar Tips [Re: BeefExtreme]
    #25844053 -

BeefSupremeJr said:
rickyswamps said:
Something that lowered my contams a lot was keeping my x-actos in cup of alcohol (outside SAB).  No need to wipe it with iso towel.  I saw it on one of Bod's videos and tried it out.  A lot better than setting the scalpel down somewhere that isn't clean.



Aye,  I saw that too.  I had a little jar of alcohol I was doing that with, but the sexy move would be to have one of those things barbers keep their blades in.

I think it’s calles a barbacide.


I always watch the scalpel pretty well when I flame so I don't light my hand on fire.  One time I put it back in the cup and the alcohol lit up.

:onfire:


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:sporedrop:Unmodded, Unflipped, No Drill, Monotub TEK:sporedrop:

:stoned:OTD - Let's Talk Music:stoned:

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Re: Agar Tips [Re: verum subsequentis]
    #25844184 -

verum subsequentis said:
Doing great man, except for the bacteria in areas. I didn't read all the posts but you are aware of the obvious bacteria, right? I'd take another transfer (smaller) of each. I think you'll be ready for grain then (assuming you don't fuck up your sterile tek). Stareatclouds is spot on with his advice. Great post, stare. If you've got specific questions feel free to ask. Otherwise we'd just have to throw out all ten million standard tips and hope we hit something you are curious about.

BeefSupremeJr said:

Try to not double dribble like that.  One culture per plate. 




Maybe good advice for a beginner just because the more times you put a transfer in the more times you risk contaminating the plate. But if you are good enough I highly advise "double dribbling". Actually i find the tripple dribble to be the best use of the available space. I only go to one dish per plate if I'm actively cleaning a filthy culture or if I'm going to use the plate for grain. Unless and until then, I triple dribble like a mofo.




Okay now that is straight handsome. 

Ssssoo, if not for grain, what are you doing with said plates?


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Need a lift?

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Re: Agar Tips [Re: BeefExtreme]
    #25844244 -

I do all kinds of shiz with plates. I did a lot of three per plate when i was working on a ridiculous penis envy isolation project. the plates in my post above were just getting cleaned up from spores. sometimes I'll put five or six (or fifteen) different cultures on one plate and throw them in cold storage while i clone hunt and or am testing out cultures.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Agar Tips [Re: badsponge]
    #25844289 -

badsponge said:
rickyswamps said:
Something that lowered my contams a lot was keeping my x-actos in cup of alcohol (outside SAB).


Do you mean for storage in between SAB sessions? Not during, right? I assume you're not taking it out of an alcohol cup and then flame sterilizing.


Why do that tho? Just curious. I don't see a point if u torch it red hot. Nothing is more sterile than red hot metal. I literally don't even think about where I put my scalpel. Just torch it and go.

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Re: Agar Tips [Re: verum subsequentis]
    #25844292 -

verum subsequentis said:
I do all kinds of shiz with plates. I did a lot of three per plate when i was working on a ridiculous penis envy isolation project. the plates in my post above were just getting cleaned up from spores. sometimes I'll put five or six (or fifteen) different cultures on one plate and throw them in cold storage while i clone hunt and or am testing out cultures.


Omg that was u? :lol: that was agonizing. Lol good work and good documentation tho.

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Re: Agar Tips [Re: nube424]
    #25844296 -

:whathesaid:

I read through that thread a couple days ago.  Wow.

Pissed me off you didn't get any good genetics out of it.


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:sporedrop:Unmodded, Unflipped, No Drill, Monotub TEK:sporedrop:

:stoned:OTD - Let's Talk Music:stoned:

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Re: Agar Tips [Re: rickyswamps]
    #25844303 -

But be proved a point that it's almost impossible (or impossible) to get a true isolate.

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Re: Agar Tips [Re: nube424]
    #25844321 -

Who says i didn't get any good genetics? I just got busy with other things. All the cultures are on slants in cold storage. It's rumored to be possible that contams are intertwined in the myc but I don't know. I just know that my initial testing all fucked off pretty hard. I'll bust em out in batches of twelve here soon and test them all out again.

"nearly impossible to get a true isolate"? Hardly, I was a noob and did things a little wrong. My next isolation thread will show how it should be done.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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