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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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How many agar plates with one disposable loop?
    #25799065 -

I have plastic disposable inoculation loops. How many agar plates can I inoculate safely with each loop? In bod's video he flames it between 1-2 plates, but I wouldn't want to waste 10-20 loops for 20 plates.

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Re: How many agar plates with one disposable loop? [Re: stash9]
    #25799072 -

unfortunately they are intended for a single use as far as "proper use" goes. That's why we use metal loops not plastic disposable loops.

You can do what you want but it will be risky to keep swiping at a print with the same loop over and over, there are nasties on the prints no matter what.


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Re: How many agar plates with one disposable loop? [Re: sandman420]
    #25799075 -

You can easily make your own loop though dont sweat it. I use ecig wire. fold it over and chuck it into a drill chuck and you can twist it really easily to make it strong enough to apply pressure.


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Re: How many agar plates with one disposable loop? [Re: stash9]
    #25799159 -

stash9 said:
I have plastic disposable inoculation loops. How many agar plates can I inoculate safely with each loop? In bod's video he flames it between 1-2 plates, but I wouldn't want to waste 10-20 loops for 20 plates.


That's exactly what you would do with disposable ones. I had a couple hundred of em lasted a few months.


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Re: How many agar plates with one disposable loop? [Re: stash9]
    #25799185 -

Alright, thanks. I have couple hundred too, and going to use them first, but I guess I'm going to make my own of metal after them. The drill chuck technique sounds good.

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Re: How many agar plates with one disposable loop? [Re: stash9]
    #25799886 -

Wire loops are really simple to make but they only cost a few bucks anyways. I bought one for 5$ and it came with a pack of nichrome wires. Twisting the loop is very simple


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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