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 stash9
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Registered: 01/27/18
Posts: 18
Last seen: 11 months, 8 hours
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 How many agar plates with one disposable loop? 
#25799065 - 02/08/19 12:20 PM (2 years, 23 days ago) |
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I have plastic disposable inoculation loops. How many agar plates can I inoculate safely with each loop? In bod's video he flames it between 1-2 plates, but I wouldn't want to waste 10-20 loops for 20 plates.
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 sandman420
Honorary Potato
Registered: 06/17/04
Posts: 2,089
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 Re: How many agar plates with one disposable loop? [Re: stash9]
#25799072 - 02/08/19 12:24 PM (2 years, 23 days ago) |
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unfortunately they are intended for a single use as far as "proper use" goes. That's why we use metal loops not plastic disposable loops.
You can do what you want but it will be risky to keep swiping at a print with the same loop over and over, there are nasties on the prints no matter what.
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Have no fear of perfection, you'll never reach it.
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sandman420
Honorary Potato
Registered: 06/17/04
Posts: 2,089
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Re: How many agar plates with one disposable loop? [Re: sandman420]
#25799075 - 02/08/19 12:26 PM (2 years, 23 days ago) |
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You can easily make your own loop though dont sweat it. I use ecig wire. fold it over and chuck it into a drill chuck and you can twist it really easily to make it strong enough to apply pressure.
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Have no fear of perfection, you'll never reach it.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 60,362
Loc: Milky way
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Re: How many agar plates with one disposable loop? [Re: stash9]
#25799159 - 02/08/19 01:05 PM (2 years, 23 days ago) |
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Quote:
stash9 said:
I have plastic disposable inoculation loops. How many agar plates can I inoculate safely with each loop? In bod's video he flames it between 1-2 plates, but I wouldn't want to waste 10-20 loops for 20 plates.
That's exactly what you would do with disposable ones. I had a couple hundred of em lasted a few months.
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stash9
Stranger
Registered: 01/27/18
Posts: 18
Last seen: 11 months, 8 hours
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Re: How many agar plates with one disposable loop? [Re: stash9]
#25799185 - 02/08/19 01:15 PM (2 years, 23 days ago) |
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Alright, thanks. I have couple hundred too, and going to use them first, but I guess I'm going to make my own of metal after them. The drill chuck technique sounds good.
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mushpunx
Fungus Punk
Registered: 04/20/14
Posts: 13,305
Last seen: 4 days, 11 hours
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Re: How many agar plates with one disposable loop? [Re: stash9]
#25799886 - 02/08/19 07:33 PM (2 years, 22 days ago) |
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Wire loops are really simple to make but they only cost a few bucks anyways. I bought one for 5$ and it came with a pack of nichrome wires. Twisting the loop is very simple
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