Caps McGee said:

Iso doesn't kill spores... just so you know

Spores can take up to 6 weeks to germinate... 3-5 days is typical IME

Even trich will take days to show: hell, a transfer from a living culture takes 24 hours to recover... patience dude

I'd probably streak the surface a short distance rather than stabbing

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Caps McGee said:

Always be attentive for contamination, even transfers from clean cultures can become contaminated by improper sterile technique... I don't worry about spots of yeast or bacteria, they can't jump,  the mycelium away from them is unaffected by them: beauty of agar work vs LC... don't worry about molds either until they change color/sporelate... it's the meshed in infections that are hard to spot... but from what I can tell by the photo, the growth from 11-12 looks clean and uniform... get a better focused photo, maybe with a brighter light behind it... if using your phone, I've found it useful to photograph from a roughly 12-18" away from the plate and crop it down to zoom


I mean fresh plate... you can go to grain, but it's an unnecessary risk IMO, especially if this is all you have going... could let it grow a bit and do both grain and fresh plates


Highly suggest making a few transfers until you see uniformity in structure and shape... like this one (which is tomentose):



This one that's rhizomorphic:



Or these clean cultures exhibiting both types of growth:

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Vibe_Enthusiast said:

Well I just wanted to pour them all so I had them for use. I'll only use like 10 out of the 25. One for cloning and some transfers and some for Ms to agar... and then transfer.

But it would be silly for me to not pour them all when I'm already doing it. So I just wanted to know if I could store the others until needed. Thanks for the replies guys.

How long do these usually stay well for unused in the fridge/shelf life

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that makes sense to pour them all. i stand corrected.

i predict shelf life depends much on how they are stored. stacked in an airtight, vacuum sealed box in the fridge is probably longest, and they'll last many weeks. (months even?) but vacuum sealing makes a huge contamination risk when the vacuum is broken, so not a good idea.

check this out:


developing my own no-pour system. sitting on all these, made the green ones yesterday, red ones are a week old. should last a couple weeks before i have to make more. stored unwrapped in generic polypropylene jars, on a shelf in the lab. there's about 4.5 dozen plates, just hangin out. point is, i'm confident in a shelf life measured in weeks.

speaking of, in response to the earlier debate, using food color to help keep track of stock rotation.

J. Jack Flash said:

Vibe_Enthusiast said:

Well I just wanted to pour them all so I had them for use. I'll only use like 10 out of the 25. One for cloning and some transfers and some for Ms to agar... and then transfer.

But it would be silly for me to not pour them all when I'm already doing it. So I just wanted to know if I could store the others until needed. Thanks for the replies guys.

How long do these usually stay well for unused in the fridge/shelf life

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that makes sense to pour them all. i stand corrected.

i predict shelf life depends much on how they are stored. stacked in an airtight, vacuum sealed box in the fridge is probably longest, and they'll last many weeks. (months even?) but vacuum sealing makes a huge contamination risk when the vacuum is broken, so not a good idea.

check this out:


developing my own no-pour system. sitting on all these, made the green ones yesterday, red ones are a week old. should last a couple weeks before i have to make more. stored unwrapped in generic polypropylene jars, on a shelf in the lab. there's about 4.5 dozen plates, just hangin out. point is, i'm confident in a shelf life measured in weeks.

speaking of, in response to the earlier debate, using food color to help keep track of stock rotation.

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J. Jack Flash said:

you'll get different answers to this, i think. i must qualify mine with: i'm not an expert.

imho, short answer is no, the plate won't last forever. the myc does kinda fill in the void, but the agar doesn't grow back. not sure how feasible it is to grab bare mycelium from the void spaces to drop into grain. i suspect it'd be too keen to stick to whatever tool you're using.

longer answer is, kinda, because you can propagate the culture, sorta, by making further transfers. but if the culture isn't a genetic isolate, each transfer narrows the gene pool. for this reason, i like dropping full half-plates from MS with fewest transfers possible, into single jars. widest genetic pool increasing chances for clone-worthy fruit. cloning, i believe, is where the 80/20 rule comes into play. getting good clone cultures going, and slanting for long term storage is where it's at.

again, i disclaim the above opinions with the fact that my experience level is pretty low compared to many here. it's based on educated reasoning and a lot of coffee. i could be way off base, and hope anyone who notices corrects me in the bluntest possible terms asap.

edit: you changed the question... absolutely take agar with it. look at the tiger drop technique. literally dropping the entire contents of the plate into a jar.

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Vibe_Enthusiast said:

One more question.. when transferring.. does it matter if some of the agar gets cut out with the myc.. or just be really careful on only getting the myc?

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Quirkmeister92 said:

Thai Lipa Yai, A2G today, each to pint masters.

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Quote:

Vibe_Enthusiast said:
what makes the myc grow both types? That's wild looking lol.

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