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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lassy123]
    #25748928 -

Left to right, Z strain variety clone specimen continuing with tomentose growth only. These were the pours I had the condensation problem with. Transfer after plate is fully grown out?
Next is MS Ecuador showing tomentose growth at 10 days...now that I’ve got the agar bug I’ll never innoc straight to grain or rice flour from MS
Last is Cam variety from MS (found an MS stash and figured best to go to agar to keep myself going on something). Tried for a sacred heart smear. Notice the yellow tint? Every plate from that sample is the same color. Has anyone else experienced that hue from MS in a variety or is it more likely it was full of bacteria?



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It looks a lot more like it does now than it did...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
    #25748947 -

Bacteria fo sho. This is why you do agar. Clean em up and get em on grain. :super:


Just arrived.


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Never give up, never surrender.

Edited by Failboat (01/17/19 10:05 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #25748966 -

Quirkmeister92 said:

Just arrived.


:hereyougo:


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #25748975 -

My thoughts...if it looks like snot, it isn’t good:) will do!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
    #25749032 -

Humanhelper said:
Left to right, Z strain variety clone specimen continuing with tomentose growth only. These were the pours I had the condensation problem with. Transfer after plate is fully grown out?
Next is MS Ecuador showing tomentose growth at 10 days...now that I’ve got the agar bug I’ll never innoc straight to grain or rice flour from MS
Last is Cam variety from MS (found an MS stash and figured best to go to agar to keep myself going on something). Tried for a sacred heart smear. Notice the yellow tint? Every plate from that sample is the same color. Has anyone else experienced that hue from MS in a variety or is it more likely it was full of bacteria?




The last dish looks horrible. There might be some mycelium at the top right corner. The 2nd dish looks salvageable, I'd transfer from 12, the z looks bacterial from the photo.


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I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Johnny Dont]
    #25749446 -

I agree to right and center but what makes you think the Z looks bacterial?  Is it the amount of fuzz?  I know I remember Caps saying in a post a while back that tons of tomentose usually indicated bacteria in his experience.  Despite the H2O droplets and off color of the pda being a sort of tannish yellow color the growth on the z is brilliant white, just hasn't started any rhizo growth. 
Let me know what you're looking at so I can learn a little more!
Gracias amigo!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
    #25749524 -

Humanhelper said:
I agree to right and center but what makes you think the Z looks bacterial?  Is it the amount of fuzz?  I know I remember Caps saying in a post a while back that tons of tomentose usually indicated bacteria in his experience.  Despite the H2O droplets and off color of the pda being a sort of tannish yellow color the growth on the z is brilliant white, just hasn't started any rhizo growth. 
Let me know what you're looking at so I can learn a little more!
Gracias amigo!


It might just be that its hard to see from the picture, but to me, it looks like you have some healthy mycelium in the middle, its nice and white, but the z looks yellow. If you can see that its actually mycelium, then obviously I'm wrong. But from here it looks like a bacterial blob


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I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Johnny Dont]
    #25749568 -

Gotcha, the pic is also a bit shit because the ol photo taker wants to focus on the water droplets.  I'll keep a weather eye on it either way. 
HH


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
    #25749591 -

So I was gonna do 3 transfers per pasty, i took 9 from the first and now I'm going to mix up more agar and pour another 18 for the next two. All the transfers!


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #25750405 -

I did agar for first time! I used single drop of Costa Rican MS spore syringe into center of agar plate. I used some prepared agar plates I got from shroomsupply because I was waiting for my pressure cooker. Oh well..they came vacuumed sealed, pack of 4. When I broke bag open, I noticed they didn't have parafilm around them! I opened it in my SAB at least. I used siran  wrap to wrap up agar dish when finished. Seemed fast. I made sure my hands weren't hovering over the plate and flame sterilized and spit out to kool down, then added a drop to center of dish and promptly closed agar dish. Took siran  and sealed it up and put it my closet along with some jars and bags I noc'ed up.
I was unhappy that agar dishes from shroomsupply didn't come with parafilm wrapped around dishes. I'll be making my own from now on

Edited by woofwoof (01/18/19 12:24 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25750410 -

How do spores get GE from agar??

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25750417 -

velvetmagician1 said:
How do spores get GE from agar??


Parafilm and plastic wrap breathe enough.


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Last seen 12/2/2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens]
    #25750423 -

I should wrap it horizontal only? I wrapped up entire plate

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25750428 -

Good for you!  Agar is awesome and cheap and easy to make.  Just curious, what did they cost?
There is enough air in the agar plates for the mycelium to grow even though the6 are wrapped.  If you want pins to form on the agar though, you do need to take the wrap off after a while.
I do not use parafilm, cling wrap is cheap and easy.  Some like parafilm but I think most prefer Saran.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25750433 -

The complete agar resource thread has tons of info and videos on working with agar in a SAB.
I wrap twice around with Saran.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25750435 -

SunnyDayze said:
Good for you!  Agar is awesome and cheap and easy to make.  Just curious, what did they cost?
There is enough air in the agar plates for the mycelium to grow even though the6 are wrapped.  If you want pins to form on the agar though, you do need to take the wrap off after a while.
I do not use parafilm, cling wrap is cheap and easy.  Some like parafilm but I think most prefer Saran.


It seemed easier than brf lol. 4 pack cost 14.95. Here is link http://www.shroomsupply.com/agar-culture-media/agar-kits/pre-poured-mea-agar-plates-set-of-four

I'm still bit confused on making my own. Gunna tackle it tho. I just wanted to get my hands some practice

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25750451 -

Ouch!  But you’re on the right track now.
:hehehe:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25750458 -

Pastywytes agar tek is most current? I need a video lol

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25750466 -

I think no pour is what I'm gunna try first

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25750646 -

velvetmagician1 said:
Pastywytes agar tek is most current? I need a video lol


Pastyplates are nice to get your feet wet and work well but you'll eventually want to pour
your own plates if you stick around. Visibility and handling is much better with petris.


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Build yourself a Flow Hood in these 99 simple steps

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