MudaFuka said:

Well Blinding leaf inspired me to make some nutrient free liquid inoculant.

Un like LC which contains nutrients and needs time to colonise. LI is made by mixing a colonised agar plate into sterile water. This is a real improvement because it greatly lowers the potential for contamination.

It also removes the need to do G2G transfers since you can simply make enough LI to inoculate as much spawn as you want right at the initial inoculation.

There are PC able blender jars on the market designed for this purpose but they are a bit pricey. So I made my own

selecting your blender

I started out with an old blender that up until now I have been using to crush drywall for gypsum. the threads on its base fit a tall half pint jar perfectly and its made of PC able plastic. It is a Procter Silex 58130R 8 speed. I really just got lucky that it works so well.

To find a blender that fits a jar. I suggest taking a jar ring to the store and seeing if it will screw on to the base of a blenders pitcher. If it dose you know it will work. As far as the plastic holding up to a PC cycle. I think most cheep blenders are likely made of the same cheep plastic. Mine looks like the same white plastic that jar lids are made of. So get a cheep white blender and chances are it will work.

Making the switch

The first step is to un screw the pitcher from its base and remove the blade assembly from the bottom of the pitcher.


Then simply put the blade assembly into a half pint jar and screw the base onto the jars top.



Now its ready to use.


Using your blender to make Liquid inoculant
Simply start by filling your jar about half way with water(tap is what I use but if you are concerned about it go ahead and use distilled). Next place the blade assembly into the top of the jar and screw on the blender base. Make sure not to tighten it all the way. It should be loos enough to provide gas exchange during the PC run.

now place it in the PC and run it at 15PSI for 15 to 20 minutes.

Once the timer goes off turn off the heat and let the pressure drop on its own. Once the pressure has dropped open the PC and quickly tighten the blender base to the jar so no contams get sucked in while it cools. I then like to put it in my SAB to cool


I like to prep my SAB before the jar has cooled so by the time the water is no longer to hot the air in the box has had time to settle.
I prep my SAB by first misting it with a mix of bleach dish soap and water then I spray Lysol in and around the box. I put my scalpel and the petri I plan on using in the SAB and I go and wash my hands and arms. 

Now that the water is nice and cool its time to go to work. Put on some gloves and coat them with alcohol or an alcohol based hand sanitizer. I also like to wear a face mask. wipe down the petri and blender jar with alcohol.


Unscrew the blender base from the jar and set it aside. I like to have an empty jar or petri in my SAB to set thing on so they don't touch the floor. Flame sterilise your scalpel(I like to use a propane torch). open up your plate and cut out the colonised portion of the agar and pick it up with the scalpel. With your other hand take the blade assembly off the top of your jar and drop in the colonised agar. Put the blade assembly back in and screw the blender base back on the jar. Threw out this process watch that your hands are never directly above the jar or plate.


Now pu the blender jar on to your blender and run it on high for about five seconds. Don't run it for to long or you risk really damaging the mycelium.


Well there you go you have a nice jar of clean liquid inoculant. You can either use a syringe to suck some up and inoculate with the syringe or just pour it in to your receiving jars like I do.

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MudaFuka said:

4 days growth.

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d0urd3n said:

You say it contains no nutrients but there are nutrients in agar. And usually only the top of the agar is colonized so there are a lot of nutrients left that contams would also like.
Thanks.

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Yeah myc only colonize the top layer, that's why you should experiment too with thin layers of agar, just when pouring don't fill the dish all the way up.