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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #18632434 -

This makes me feel better about trying agar great write up. Loved it.

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Re: Stro's Cleaning and Isolating On Agar [Re: Crispyth]
    #18691273 -

just posting to put in my threads :smile: starting agar soonish very nice write up though

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Re: Stro's Cleaning and Isolating On Agar [Re: vaneazy]
    #18691390 -

Im happy to see more of these threads poppin up. When I started agar I looked for something like this and couldnt find much so I was posting questions about sectors and making iso's. this would have saved alot of time...A must read for beginers with agar!!


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Re: Stro's Cleaning and Isolating On Agar [Re: first time expert]
    #19218374 -

Nice write up, bump for relevancy and so that it's in my threads to use as source material


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Stro's Cleaning and Isolating On Agar [Re: bodhisatta]
    #19224628 -

Oh man thanks for the time and effort you put into that. Great read, definitely helped me out as this is exactly what I've been working more on recently.

Some notes and then a ?. In the pictures used showing 7 days after first transfer you will notice for both plates A and C that the growths for the most part are mostly cottony. Looking at the 11 days after pics you can see in them the same original cottony growth which after a certain amount of growth it develops more characteristic and rhizomorphic hyphea in some places. I believe you noted in the tek that the myc has now had time to mature and actually develop rhizomorphic hyphea at this point which is exactly what i want my question focused on. Right now I am using the same 1/2 pint wide mouth jars recommended for BRF cakes as my ajar plates. This has allowed me to reuse the same containers over and over and gain enough skill working with it to hopefully just go ahead and invest in actual agar plates. That point aside...

Is it normal for myc to grow out in that way... where the initial growth comes out more fluffy and as it matures the rhizo growth is able to form and sectoring and stuff like that become much more obvious? I ask because after reading this tek it made me wonder if perhaps all my plates simply appear so cottony because there is far less surface area for the myc to grow out and mature on like it would on a larger plate. Not a single plate that I can think of growing out over the last few months has developed nearly any "obvious" rhizo growth and its made me question a lot of different things cus well... I'd like to master the art as much as possible.

Part of the issue was likely that the agar recipe I used was an old Doc_T one that I found which worked perfectly fine for growing out clones or using colonized pieces of grain but i guess was just to solid for any sort of spore germination at all. I recently made the switch over to the same 500-10-10 you mention using which has had great success with germinating spores and well im waiting to see how the actual growth decides to come out.

SO - Would you say that those 1/2 pint wide mouths offer sufficient amount of space for myc to actually grow out and mature enough to where someone could hopefully see some solid rhizo formation or am I pushing it?

I ask because Im looking into possibley changing containers and figured a little input on the potential of this even being a factor or not is real or just something ive over thought?


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"Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"



"All that is gold does not glitter, Not all those who wander are lost"


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Re: Stro's Cleaning and Isolating On Agar [Re: Mosey3012]
    #19226205 -

Very useful and helpful thread, great illustrations! Thanks for posting this information.

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Re: Stro's Cleaning and Isolating On Agar [Re: budkatz]
    #19226284 -

Banging Write up bro

:mushroom2::mushroom2::mushroom2:
:mushroom2::mushroom2::mushroom2:


Any chance you can show something so beautiful with Pans?...


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"wise words here"
:toadkart:

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Re: Stro's Cleaning and Isolating On Agar [Re: budkatz]
    #19226287 -

Great guide

Isolating away from bacteria can be hard sometimes I read

thats is why the sandwich method or hot pour (on top of mycellium) was invented iirc

sometimes the bacteria will ride on the mycellium and be almost impossible to isolate away from

but I have had good success with isolating away from bacteria without once

the cause of bacteria was a biopsy too....
easy methods arent always the best :-)

agar is the only way to get stable results, anyone growing edibles should do it

oatmeal agar is an easy way to get started.. mycellium loves oatmeal agar


sometimes it is easier to start all over from sporeprint if it is hard to get rid of the bacteria
that way you can be pretty sure there is no bacteria in your dish..
only mold, and mold is easy to isolate away from

isolating away from bacteria can be hard.. antibiotic agar/hot pour/sandwich teks

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Re: Stro's Cleaning and Isolating On Agar [Re: Stool_Sample] * 1
    #19237464 -

Mosey3012 said:
Would you say that those 1/2 pint wide mouths offer sufficient amount of space for myc to actually grow out and mature enough to where someone could hopefully see some solid rhizo formation or am I pushing it?


Although genetics play the dominant roll, there are also many other factors that determine the growth type of your culture (rhizomorphic or tomentos). Many of times I have seen cultures start out strong and fast with rhizo, but also vice versa. As long as conditions are suitable it will continue to adapt and grow, I honestly can't tell you how much these conditions affect the uniformity, appearance, or class of mycelia it displays. I will however say that regardless, you should go ahead and move on to dishes/plates, as there are many benefits to doing so. Also, when you are starting out, try to be consistent with your agar formula and temps once you get it set up, knowing how the culture respond to it will help you when working with them.

Stool_Sample said:
Any chance you can show something so beautiful with Pans?...


This photo was taken after a sector was removed from it, it was photoshopped back in just for you. ; )



mio said:
thats is why the sandwich method or hot pour


I've used that method quite a bit, especially when working with stuborns and wilds.


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Current Grows

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The Noob Forum
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The Tribe

Edited by Stropharis (12/06/13 05:04 PM)

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #19237472 -

hey stropharis! nice to see ya around again:thumbup:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
    #19237484 -

Just stopping by, I'm hoping to be back at it in March, we will see.


--------------------
Current Grows

Stro's Write Ups
The Noob Forum
Amateur Mycologists United
The Tribe

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #19237490 -

ok well phone first next time:shakefist:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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to the question about agar plates vs ½pint wide mouth jars for agar [Re: cronicr]
    #19238687 -

½ pint wide mouth works good IME, got a mono culture from 2-3 transfers from a clone it seems

now if I had taken the biopsy better and not been lazy/hurrying so much etc. it might be possible in 1 transfer

plates are just much better/more enjoyable to work with I would imagine
just be sure to get vented dishes or your culture dies

the advantage of jars for agar is you can PC them with the agar in... dishes cant be if they are plastic
(you also save the expensive parafilm, but parafilm can be substituted with cellophane wrap, it is breathable too)

larger dishes arent necessarily better, they can be harder to work with I heard
some prefer 120mm, some 80mm or so

glass dishes are harder to work with than plastic often if you want to stack them like is usually done

the biggest problem with jars IMO is that they are hard to stack after transfers
if you want to do 5-6 transfers your still air box might be over-crowded very quickly

plastic petri dishes are much better if you are serious about isolating/doing agar work IMO

Edited by lessismore (12/06/13 10:11 PM)

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Re: to the question about agar plates vs ½pint wide mouth jars for agar [Re: lessismore]
    #19333586 -

Great write up. Thank you.


--------------------
How Frank gets shit done
RR's notes
RTB Forum

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Re: Stro's Cleaning and Isolating On Agar [Re: Chimaira]
    #19333816 -

Awesome thread man...very informative!!!  Thanks for this:thumbup:


--------------------
~~Everything is relative~~

A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.

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Re: Stro's Cleaning and Isolating On Agar [Re: 1down5up]
    #19335069 -

1down5up said:
Awesome thread man...very informative!!!  Thanks for this:thumbup:


:whathesaid:

This one can get some more front page time.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Stro's Cleaning and Isolating On Agar [Re: bodhisatta]
    #19338701 -

:asianofapproval:
This is a beautiful writeup. Thanks bro!

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Re: Stro's Cleaning and Isolating On Agar [Re: Vis0nairee]
    #19454264 -

Very nice guide!:thumbup:

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #19654252 -

Stropharis said:
Just stopping by, I'm hoping to be back at it in March, we will see.


it's march, i didn't forget lol! bumpity bump stropharis where ya at!


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
    #19654584 -

Feel like im in with the cool kids now with this knowledge :P. Thanks great guide ill be puttin it to use soon!

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