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simonjester44
Old AF



Registered: 03/22/13
Posts: 94
Loc: EAST COAST
Last seen: 11 months, 5 days
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Potency, Monotubs, and mono-cultures.
#18861986 - 09/19/13 08:39 AM (10 years, 4 months ago) |
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There will be a couple of questions contained in this post.
The picture you see is a selection of fruits from a monotub grow a-la a modified Ohmatic monotub (25 gal) and good-old Damion5050 coir Tek.
...just for reference: I used 2x coir recipe per tub to yield 6 inches of substrate in each 25 gal tub....
Strain:
I received a wedge from a friend that was labeled John Allen (F2). I pout the wedge on a plate, and it colonized nicely without sectoring. I transferred from that plate to other plates, colonized, and then used an entire plate (F4) to colonize some WBS. I fruited them in a small monotub.
Because I did not have much experience with cloning. I decided to clone one particularly fat little fruit, and then I used that clone to then colonize more plates witch ultimately went into colonizing more WBS for the grow you see pictured.
Here are my questions:
Strain:
This is a mono-culture right?
Potency:
Can the potency in a mono-culture vary from fruit to fruit like they can in MS?
If my personal QC test indicate high potency, should I expect any variation within the same monotub?
Any comments would be great. I've gotten some mixed reviews, but I absolutely lost my mind one day on one 3g cap.
-------------------- "Never underestimate the power of friction as a construction adhesive."
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Potency, Monotubs, and mono-cultures. [Re: simonjester44]
#18862233 - 09/19/13 09:50 AM (10 years, 4 months ago) |
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If and only if your culture from that friend was a monoculture/isolate will the clone you took from the tub also be an isolate. If the culture you got from your friend was a multispore or a clone itself (limited but still multiple genetics) the fruit you cloned could itself be all of those genetics or some of them or maybe in an extremely rare case a monoculture.
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 9 months
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Re: Potency, Monotubs, and mono-cultures. [Re: simonjester44]
#18862350 - 09/19/13 10:14 AM (10 years, 4 months ago) |
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Quote:
simonjester44 said:
I received a wedge from a friend that was labeled John Allen (F2). I pout the wedge on a plate, and it colonized nicely without sectoring. I transferred from that plate to other plates, colonized, and then used an entire plate (F4) to colonize some WBS. I fruited them in a small monotub.
This shows the fact that its an isolate, at this point all your future clones are the same iso just farther towards senescence.
The potency will be consistent with a mono.
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
Edited by PrinceShroom (09/19/13 10:16 AM)
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simonjester44
Old AF



Registered: 03/22/13
Posts: 94
Loc: EAST COAST
Last seen: 11 months, 5 days
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Re: Potency, Monotubs, and mono-cultures. [Re: bodhisatta]
#18862368 - 09/19/13 10:19 AM (10 years, 4 months ago) |
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Thanks for the reply guys!
My friend has been in the hobby for years, so I would doubt he sent me a MS.
Can I assume that no sectoring on the plate = isolate? Can I assume that after, say 2-3 transfers without sectoring, that I've got a good isolate?
-------------------- "Never underestimate the power of friction as a construction adhesive."
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 9 months
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Re: Potency, Monotubs, and mono-cultures. [Re: simonjester44]
#18862376 - 09/19/13 10:21 AM (10 years, 4 months ago) |
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Quote:
simonjester44 said:
Can I assume that no sectoring on the plate = isolate?

As soon as you see that its not sectoring you start using it. The more plates you transfer the culture to the older it gets.
--------------------
  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
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PrinceShroom
Experienced Mofo



Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 9 months
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Re: Potency, Monotubs, and mono-cultures. [Re: PrinceShroom]
#18862384 - 09/19/13 10:24 AM (10 years, 4 months ago) |
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The F2 after John Allen on your plate is probably like stamet's P value. That would be the 2nd fully colonized plate from the isolate. The further up the value you go the older the culture gets. Cubes can go 6-8 before senescence hits IME.
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  WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Potency, Monotubs, and mono-cultures. [Re: PrinceShroom]
#18862669 - 09/19/13 11:39 AM (10 years, 4 months ago) |
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Quote:
PrinceShroom said:
Quote:
simonjester44 said:
I received a wedge from a friend that was labeled John Allen (F2). I pout the wedge on a plate, and it colonized nicely without sectoring. I transferred from that plate to other plates, colonized, and then used an entire plate (F4) to colonize some WBS. I fruited them in a small monotub.
This shows the fact that its an isolate, ...
No, it doesn't. Sometimes sectoring is not visible until after 1 or more transfers.
Perhaps the label indicates its supposed to be an isolate, but that's just a question of trust.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Potency, Monotubs, and mono-cultures. [Re: SpitballJedi]
#18862684 - 09/19/13 11:45 AM (10 years, 4 months ago) |
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Quote:
SpitballJedi said: Perhaps the label indicates its supposed to be an isolate, but that's just a question of trust.
This is what I was getting at in the very first reply.
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