Home | Community | Message Board


Kratom Eye
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Malt Extract, Paul Stamets, Petri Dish, Potato Dextrose, pH Test Strips

Jump to first unread post. Pages: 1
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
my first agar project
    #3622601 - 01/13/05 10:23 AM (11 years, 10 months ago)

hi guys, i read lots of recipes for preparing agar plates....
i want you to tell me if the procedure is correct,i won't tlak about quantities of each ingredient. just the procedure.
ingredients: Agar agar, Potatoes, Dextrose,petri dishes

procedure:
i melt the potatoes in a pot and boil them in water i then only keep the liquid.
I put this liquid in a bowl and add agar agar and dextrose and mix them.
I sterilize this mix for 20'
I let it cool slowly....
and i pour into the plates...

please tell me if i am wrong somewhere in the procedure
questions:
1)dextrose i can find in a drug store in powder form.is this what we want?
2)if i have more liquid than the petri dishes,how could i keep it alive for a few weeks/months?

thank you all!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,898
Last seen: 1 year, 28 days
Re: my first agar project [Re: Hamurabi]
    #3622705 - 01/13/05 11:11 AM (11 years, 10 months ago)

I've heard that agar can last months regardless...
But i don't know to much about agar,

If you take on the ocean, you'll be sure to drown....-GTA"The Truth"
Haha


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: my first agar project [Re: Hamurabi]
    #3622980 - 01/13/05 01:08 PM (11 years, 10 months ago)

Quote:

Hamurabi said:
hi guys, i read lots of recipes for preparing agar plates....
i want you to tell me if the procedure is correct,i won't tlak about quantities of each ingredient. just the procedure.
ingredients: Agar agar, Potatoes, Dextrose,petri dishes

procedure:
i melt the potatoes in a pot and boil them in water i then only keep the liquid.
I put this liquid in a bowl and add agar agar and dextrose and mix them.
I sterilize this mix for 20'
I let it cool slowly....
and i pour into the plates...

please tell me if i am wrong somewhere in the procedure
questions:
1)dextrose i can find in a drug store in powder form.is this what we want?
2)if i have more liquid than the petri dishes,how could i keep it alive for a few weeks/months?

thank you all!




Never done the homeaide agars , but sounds about right .
Personally would pc for 40 mins , but that is me .
Yes if you make too much , just store in the fridge,in a glass botle with narow neck, with a very good airtight lid , and when ready to use again just put in pot of hot water till easy to pour , can store in fridge easily for a few weeks to a month .
GL
PS TMC has a couple of recipes for making own agars .Also remeber when making own agars , one should periodically check ph of the mixture .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblederx
who run it
 User Gallery

Registered: 05/30/03
Posts: 2,459
Loc: dx/dt
Re: my first agar project [Re: tripndicular]
    #3622996 - 01/13/05 01:13 PM (11 years, 10 months ago)

trip, how in fact do you do agar if you don't make your own? Have you boughten over 200 pre-sterilized petri dishes with agar in them?


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 11 months, 22 days
Re: my first agar project [Re: derx]
    #3623008 - 01/13/05 01:16 PM (11 years, 10 months ago)

LOL derx revenge :sniper:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: my first agar project [Re: derx]
    #3623020 - 01/13/05 01:21 PM (11 years, 10 months ago)

Quote:

derx said:
trip, how in fact do you do agar if you don't make your own?  Have you boughten over 200 pre-sterilized petri dishes with agar in them?




Holy mother of god , you are determined to proove me a liar huh ???
Have you ever heard of pre made agar in powder form ????????????????
Sporeworks and many many other places sell it , check it out !
And if I wanted to spend that kind o money , yes one could buy pre poured petris with agar in them , but I add water , pc , and pour .
And to you ,for now the name is MR Tripndicular , not trip !

ps , just for you , I may ask friend to take pics (no face included) while I make , pour , and inoc , tissue transfer etc ..... WITH NO GB OR FH ! Will take some time to prep and make sure friend is clean enough (lots of greasy hippy friends  :smirk:) to help me ! Why oh why do I feel the need to proove myself ?

re-edit , nor do I use peroxide in the mix .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Edited by tripndicular (01/13/05 06:37 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,103
Loc: London Flag
Last seen: 10 months, 3 days
Trusted Cultivator
Re: my first agar project [Re: Hamurabi]
    #3623699 - 01/13/05 04:25 PM (11 years, 10 months ago)

sounds pretty good. dice the spuds up small and keep the skins on - then cook em 20 mins and pour it through a sieve, keep the liquid.

add water to the volume your recipe says, and add the dextrose (yes, the stuff in the chemists) and the agar (chinese shops sell lots of this). some recipes also add yeast, or yeast extract. pc in a glass container with a chunk of polyfil in the top, and don't fill it all the way up, as it can boil and bubble. 20 minutes should do it.

open the lid when it stops hissing but is still hot. to germinate spores, it's now ready to pour; but to grow mycelium from mycelium (ie. wedges) you want to add some peroxide after it cools below 140 and before it cools to setting temperature (around 125). you need a 'candy thermometer' for this. don't worry - it takes bloody ages to cool down when you're waiting for it.

the good thing about peroxide agar is that it does keep for ever. i make up litre batches of PDA, pour all the plates i want, then stuff polyfil in the bottle neck and just leave it on the sideboard. stays for months, until i want some more plates - this time i just put the solidified agar-bottle in the pc and give it a little longer, maybe 40 minutes, so it melts. of course, it needs new peroxide each time, as it denatures above about 145.


agar is fun - look forward to trading wedges with ya, hamurabi  :laugh: :thumbup:


--------------------
buh


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: my first agar project [Re: shirley knott]
    #3623721 - 01/13/05 04:31 PM (11 years, 10 months ago)

if you wanna do some nice agar,
id advise you to try my beer agar project aswell.

click the link in the signature,
it goes all about peroxiding and how to prepare it in detail.

and you dont need malt extract.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: my first agar project [Re: ohmatic]
    #3623785 - 01/13/05 04:50 PM (11 years, 10 months ago)

thank you guys,u really helped:-)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: my first agar project [Re: shirley knott]
    #3624284 - 01/13/05 06:44 PM (11 years, 10 months ago)

This is best way to make and store agar IMHO

Insertion of syringe needles to act as a vent ...


Needles in place ready to stick in the PC ...


Of course one pulls the needles prior to storeage , those are just to act as vents while in the PC . And yes that is just water in there , this is an example of a way to make and know stays clean in the fridge , and no worries with water soaking cotton or poly etc .


Just an idea for ya  :wink:


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,378
Re: my first agar project [Re: tripndicular]
    #3624374 - 01/13/05 07:04 PM (11 years, 10 months ago)

Here is the agar recipe I use and the instructions.

http://www.mykoweb.com/articles/cultivation.html

:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: my first agar project [Re: Peterthinks]
    #3627510 - 01/14/05 08:40 AM (11 years, 10 months ago)

thanks!!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLaughingJim
Anti-Toxin
 User Gallery

Registered: 09/30/04
Posts: 465
Loc: USA, ( CT )
Last seen: 11 years, 2 months
Re: my first agar project [Re: Hamurabi]
    #3628518 - 01/14/05 03:03 PM (11 years, 10 months ago)

I find it funny that there is a beer-tek for agar...

I use beer to promote moss growth... Just raw beer! Works great! Actualy, the starch and yeast method has been used for hundreds of years. The ancient chinese would pour all of thier water used to clean rice, all over thier gardens and stones/statues. Within weeks-months, you would see massive moss growth, thick and bright green. Never needs mowing, looks better than grass, cleans the water that passes through it, and stays green all winter!

They would do the same with any fermented beverages/foods (Grain alcohol, not really beer!)

http://members.rediff.com/archanasin/kinkaku.htm

Here is a cool one in Queensland... I don't think beer was part of it though!
http://www.australianlandscapes.com/The_Photographers/Barry/Barry_s_Page/Moss/moss.html


--------------------
Don't forget to RATE us if you think we are offering GOOD or BAD advice, Thank-you!

I participate in the PROTEIN FOLDING PROGRAM helping the shroomery to help others,
FOLDING.STANFORD.EDU
I support www.shroomery.org (Team # 12679)


Edited by LaughingJim (01/14/05 03:10 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Malt Extract, Paul Stamets, Petri Dish, Potato Dextrose, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* making agar dishes using dried potato skins Dragonaut 719 4 06/18/05 01:15 AM
by ezozo
* Agar questions WhiteBunny 739 13 07/18/08 12:23 PM
by chobumms
* Easy PFA Agar Recipe HerbBaker 709 1 02/03/08 03:06 PM
by tahoe
* odwalla carrot juice agar question. doze 300 2 01/28/09 10:44 PM
by doze
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 1,905 8 02/14/05 09:57 AM
by Roadkill
* Weird LC recipe
wollongong
856 4 06/26/07 07:20 PM
by CaptainLinger
* Quick agar question johnny_poke 690 6 01/30/09 01:40 PM
by RogerRabbit
* potato starch for agar? artizen 3,034 5 01/29/09 08:21 PM
by CheeWiz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,568 topic views. 27 members, 106 guests and 22 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mycohaus
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.062 seconds spending 0.003 seconds on 14 queries.