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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Hydrogen Peroxide
    #842302 - 08/25/02 01:33 PM (22 years, 1 month ago)

Has anyone here heard of using hydrogen peroxide as a sterilizing agent verses using pressure cooker etc...... ?
I am new to forum and growing , I have been doing a lot of research , and in my exploits have found some mention of a method using peroxide .
:


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Offlineprimitive
newbie
Registered: 06/10/02
Posts: 38
Last seen: 6 years, 4 months
Re: Hydrogen Peroxide [Re: tripndicular]
    #842313 - 08/25/02 01:38 PM (22 years, 1 month ago)

no i have never heard of a tek only using hydrogen peroxide only. i know that hydrogen peroxide wont hurt the mycellium but i think it will kill spores.

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InvisibleRipple
Ripple
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Folding@home Statistics
Registered: 05/16/02
Posts: 21,014
Loc: the timbers of Fennario
Trusted Cultivator
Re: Hydrogen Peroxide [Re: tripndicular]
    #842314 - 08/25/02 01:39 PM (22 years, 1 month ago)

Read a bit more my friend.....the easy answer is no


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The bus came by and I got on that's when it all began!


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Offlinetchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 10 years, 17 days
Re: Hydrogen Peroxide [Re: tripndicular]
    #842375 - 08/25/02 02:28 PM (22 years, 1 month ago)

i think you are perhaps confusing pasturize and sterilize. hydorgen peroxide is not effective against large colonies of bacteria or multicellular organisms like mushroom mycelium. do a search on the subject, there are thousands of posts on it.

Edited by tchyted (08/25/02 02:29 PM)

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Hydrogen Peroxide [Re: tchyted]
    #842641 - 08/25/02 05:07 PM (22 years, 1 month ago)

No, according to the site I found it at , it is a method that is new to the edible mushroom community i.e. morrells , portobellos etc........ they mention the use in composted materials . I am not sure if method can be used in the growth of P.CUBES . They also mention the lack of normal equipment needed with the method .
That is why I have come here hoping to find someone with experience in growing my favorite species , that may understand the basic info described at web site I found . I do not want to buy from them if it cannot be applied to my project .
By the way , I am not expreienced at all in growing any fungi except athletes foot hahaha .


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Offlinewrestler_az
PsiLLy BiLLy
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Registered: 08/11/02
Posts: 13,685
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Re: Hydrogen Peroxide [Re: tripndicular]
    #842643 - 08/25/02 05:09 PM (22 years, 1 month ago)

what site?


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how's your WOW?





  Edited by yageman (04/20/06 4:20 PM) 

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Hydrogen Peroxide [Re: wrestler_az]
    #842652 - 08/25/02 05:10 PM (22 years, 1 month ago)

mycomasters.com


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Offlinejimsuzo
I am the Eggman

Registered: 08/14/02
Posts: 269
Loc: Land of the not-so-free
Last seen: 14 years, 10 months
Re: Hydrogen Peroxide [Re: tripndicular]
    #842719 - 08/25/02 05:43 PM (22 years, 1 month ago)

Peroxide is NOT a substitue for pressure cooking as it will kill the spores that come into contact with it. The most popular use of peroxide is either 1) spraying in a dilute solution on cased substratesor 2) for adding to the water that one adds to a perlite-floored terrarium. In either use, the ratio used is roughly 10 parts H20 to 1 part peroxide. It basically is good for killing and preventing fungus/mold. Hope this helps...


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There are roughly nine galaxies for every person alive on the planet today. Each of these galaxies has a billion suns, give or take the odd hundred million

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Hydrogen Peroxide [Re: jimsuzo]
    #842801 - 08/25/02 06:14 PM (22 years, 1 month ago)

Thanx , does help a bit . I will try by the rules of kit I am to get , then possibly buy the method to experiment with . It sounded as if it would help with contams alot without a lot of effort , and equipment . Of course they only say enough to interest you , not enough to apply it though .
Are you experienced in mycology ?


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: Hydrogen Peroxide [Re: tripndicular]
    #842816 - 08/25/02 06:19 PM (22 years, 1 month ago)

In reply to:

in my exploits have found some mention of a method using peroxide


You probably read about adding a bit of peroxide to agar or even mixing peroxide with spray water. Do a search for more info if you're curious.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Hydrogen Peroxide [Re: mickey_rourke]
    #842874 - 08/25/02 06:35 PM (22 years, 1 month ago)

No , it was an add of a sort , check it out , I need to read the site more for clues . I did a search on MSN for.......... mushroom "growing " , I found mycomasters.com .
If you have experience , please see what you can extract . I am not knowlegable in area ....yet !


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: Hydrogen Peroxide [Re: tripndicular]
    #842913 - 08/25/02 06:50 PM (22 years, 1 month ago)

If I'm not mistaken (haven't read the papers), those documents describe using peroxide to inhibit contaminants in your processes, not the substrates that you innoculate.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Offlinegroingrinder
mycophiliac

Registered: 05/22/02
Posts: 271
Last seen: 18 years, 11 months
Re: Hydrogen Peroxide [Re: tripndicular]
    #842966 - 08/25/02 07:22 PM (22 years, 1 month ago)

I have heard of adding 10cc of peroxide to quart jars of grain when doing grain to grain transfers.


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Let's not confuse truth with reality.

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Offlinegoatywoaty
THing of thing

Registered: 04/11/02
Posts: 263
Loc: caught in the storm of en...
Last seen: 21 years, 2 months
Re: Hydrogen Peroxide [Re: jimsuzo]
    #843373 - 08/26/02 02:18 AM (22 years, 1 month ago)

Peroxide is NOT a substitue for pressure cooking as it will kill the spores that come into contact with it.

hrmn i added a bit o hydrogen peroxide to my pf jars before i sterelized them. will this make a difference later when i innoculate? im thinking yes, but tell me if im mistaken. i shure hope im not heating up sterile jars lmao... (newb if you cant tell)


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[real.eyes.realize.real.lies]
the only reality is the one you make of it, for in the end it becomes what it always was.
______________________________
:::anything above this line is a lie:::


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Hydrogen Peroxide [Re: mickey_rourke]
    #843715 - 08/26/02 07:17 AM (22 years, 1 month ago)

They also mention using at prep of substrates . But I just scanned it some more , they seem to revolve around the use of paper pellets , wood fuel pellets etc .... have not found info on if applicable to compost grown mushrooms .
Definetly requires some investigation , experimentation .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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OfflinetelefunkenU47
numerator
Registered: 08/03/02
Posts: 102
Loc: cartoon planet
Last seen: 21 years, 1 month
Re: Hydrogen Peroxide [Re: goatywoaty]
    #844257 - 08/26/02 11:45 AM (22 years, 1 month ago)

it may have just been a fluke, but i recently had a successful inocculation with a syringe containing spores, water and h2o2. i had a rather large fruit that did not dry fast enough in the chamber and sporulated on some foil. i put the foil in sterile water with a small dash of peroxide, shook, drew into a syringe and innoculated a jar that had a very small patch of halted growth on one side (i innoculated on the other side). i checked the jar 7 days later to find a massive amount of mycelium on the h202 spore solution side. as there was already mycelium in the jar, i almost suspect that the jar was dehydrated and just needed more water. in any case, i have recently duplicated the experiment with a jar that has never been innoculated previously. i'll post an update on my findings in a few days.


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Central Scrutinizer:
This is the CENTRAL SCRUTINIZER...
That was Joe's first confrontation with The Law.
Naturally, we were easy on him.
One of our friendly counselors gave him
A do-nut...and told him to
Stick closer to church-oriented social activities.

-Frank Zappa's "Joe's Garage"

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Invisiblesoochi
Chef
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Registered: 08/13/02
Posts: 2,420
Loc: The Richest County
Re: Hydrogen Peroxide [Re: tripndicular]
    #863930 - 09/04/02 03:52 PM (22 years, 1 month ago)

In reasonable amounts h2o2 is completely safe on mycelium and is more commonly used to mist casings, when h2o2 is exposed for a prolonged time to sunlight and air it loses an oxygen molecule rendering it h2o which we all know is as safe as water!



got it?


--------------------
Wee, sleeket, cowran, tim'rous beastie,
O, what panic's in thy breastie!
Thou need na start awa sae hasty,
Wi' bickering brattle!

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