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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal] * 3
    #29726106 -

Thank you so much. For the link. I am amidst of taking an exam in computer engineering so I am short of time to research on my own. Deeply appreciated!

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Re: Ask Quick Questions, Get Quick Answers [Re: sozu] * 1
    #29726146 -

Cubensis can and have been known for having hollow stipes(stem). We try to breed away from this. So that's what someone means when they say 'solid stipe'.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: fontinalis666] * 1
    #29726228 -

fontinalis666 said:
Those are just misplaced hyphal knots, nothing to stress over



Alright,  I guess I've just been second guessing the hell out of them. I've never minded side pinning too much. Hopefully some turn into pins after the weekend.  I suppose this is nothing more than some APE mutant-shenanigans going on. :ape:


Thanks for your input!


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Re: Ask Quick Questions, Get Quick Answers [Re: Doozberry] * 1
    #29726295 -

Ok this is probably a dumb question, possibly covered elsewhere, but a lot of folks like to use a leather punch or mycology punch to get those cool perfect round transfers.

I struggle with them.

For one thing, getting the punch I have red hot means a LOT of metal is red! It's an actual mycology punch from a vendor (think it is originally a dental tissue punch but sold as mycology punch).

Anyways, so I flame the thing and it takes about 2-3 seconds and a LOT of metal is red hot at that point. So I wait until it starts to go dark again, 2-3 seconds, inside the SAB.

I'm worried about adding a contam vector if I just use an empty plate to cool it off. I've seen some guys say they don't bother cooling their knives on transfers, but I tried it and the damn thing is still super hot even after it's no longer red hot.

I think I've killed some transfers this way.

I also have a fancy mycology punch from Etsy with a plunger to expunge the transfer. But it, too, is a LOT OF METAL to heat up, and I end up with the same problem. Cool concept, but there's a lot of metal to heat up to red hot to get the outer and inner parts red hot.

If I use the transfer plate itself as a cooling place, then the transfer plate is all mushy and lumpy and looks nothing like what the cool kids are doing.

And I've heard that just using 70% isopropyl alcohol is a bad idea for this sort of thing, as I'm tempted to just plunge the red hot transfer punch into a small container of it. But that's a no-no I hear.

So, how's a fella s'posed to get those lovely perfect transfers, without also adding contams or mucking up the transfer plate?

Thanks for any expertise. I'm using an SAB and a flame torch.

Mike


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Mycelium? It's not MYcelium! It's YOURcelium!

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol] * 2
    #29726301 -

Not an answer to your question, but a tidbit that someone might appreciate anyhow.

My exacto knife handle is like 100% aluminum and heat sinks wayyy to well so I've taken to just using my 18g hypodermic needles that I flatten the end half of as low cost disposable and rapid to heat/cool blades.


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β€ƒπšƒπš‘πšŽ πšŒπš›πš’ 𝚘𝚏 πšπš‘πšŽ πšŒπš’πšŒπšŠπšπšŠ   π™Άπš’πšŸπšŽπšœ 𝚞𝚜 πš—πš˜ πšœπš’πšπš—   πšƒπš‘πšŠπš πš™πš›πšŽπšœπšŽπš—πšπš•πš’ πš’πš πš πš’πš•πš• πšπš’πšŽβ€ƒ
 :flowers: π™Όπš’ π™»πš’πš—πš”πšœ :hi:  :mushroom2: π™ΆπšŽπšπšπš’πš—πš πš‚πšπšŠπš›πšπšŽπš π™΄πšœπšœπšŽπš—πšπš’πšŠπš•πšœ :earth:  :happyheart: πš„πšœπšŽπšπšžπš• π™»πš’πš—πš”πšœ :bow2: 
Trade List SoonHitchhiker's Guide to the Shroomery 1-2Agar Island Clean up
The Rescue CrewQuick Questions Answered HereEz Dial Mono Tub
π™²πšŠπš”πšŽ πš†πšŠπš›πšœ 𝙸𝙸𝙸 π™·πšŠπš™πš™πšŽπš—πš’πš—πš π™½πš˜πš β€ƒ|β€ƒπ™·πšŠπš™πš™πš’ π™·πšŠπš•πš•πš˜πš πšŽπšŽπš—

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Re: Ask Quick Questions, Get Quick Answers [Re: Cicadoidea] * 1
    #29726322 -

@mychol

My go-tos are zit popping tools from Amazon, which I sterilize in sterilizing pouches, in groups of 3 normally. Like you can see here



They have two ends, all of them work for getting samples, some are better than others, and none will have perfect clean round circles.

This way, I use one end, use it for one plate/variety, and if I was careful, I can flip it over and use the other end if it didn't touch anything.

I like sterilizing the tools entirely, I just open it up in the SAB, and keep the plastic layer on after picking my first tool. They never leave the sab because I don't bother flame sterilizing, unless I ran out of tools. They get hot though throughout the entire body.

Doesn't mean flame sterilizing isn't viable, but you'd probably be better off with something like a thin scalpel or exacto knife. Needles are also viable, get red hot easy, make a lil square then poke it out.

Does anyone have like a "staff" to add needles to to make it a mycology tool, or does everyone just use a syringe itself?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol] * 3
    #29726324 -

I have an induction heater and most stainless and aluminium isn't magnetic enough to trigger my induction coil which means whatever I use would take a long time to get red hot, now, that's not necessary at all though. One second in the induction heater is enough, if I put it in my glass of iso it boils.

That's not even the main problem, the problem is waiting for it to be cool enough to not kill my transfer, who has time to wait several minutes for a tool that in the end just make a punch and I would have to do the actual transfer with my scalpel anyway :waits:

I have a FFU and don't really need to worry about leaving my scalpel for a however long I want, but in a SAB as soon as the punch or blade is cold contamination can land on it and the transfer is fucked :uhoh:

I do agree that a perfect round transfer looks very nice and if it was for a agar picture  competition I could see why one would want to do it, but for everything else an agar punch can

:begone:


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β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29726370 -

The solution to your problem is an easy fix. Be patient and let it cool down a bit.

Solved.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29726373 -

Cicadoidea said:
Not an answer to your question, but a tidbit that someone might appreciate anyhow.

My exacto knife handle is like 100% aluminum and heat sinks wayyy to well so I've taken to just using my 18g hypodermic needles that I flatten the end half of as low cost disposable and rapid to heat/cool blades.


That's an interesting idea - thank you!

Huskies said:
@mychol

My go-tos are zit popping tools from Amazon, which I sterilize in sterilizing pouches, in groups of 3 normally. Like you can see here



They have two ends, all of them work for getting samples, some are better than others, and none will have perfect clean round circles.

This way, I use one end, use it for one plate/variety, and if I was careful, I can flip it over and use the other end if it didn't touch anything.

I like sterilizing the tools entirely, I just open it up in the SAB, and keep the plastic layer on after picking my first tool. They never leave the sab because I don't bother flame sterilizing, unless I ran out of tools. They get hot though throughout the entire body.

Doesn't mean flame sterilizing isn't viable, but you'd probably be better off with something like a thin scalpel or exacto knife. Needles are also viable, get red hot easy, make a lil square then poke it out.

Does anyone have like a "staff" to add needles to to make it a mycology tool, or does everyone just use a syringe itself?


That's a cool way to do it. I have some dental tools too that I've used.

Kloakk said:
I have an induction heater and most stainless and aluminium isn't magnetic enough to trigger my induction coil which means whatever I use would take a long time to get red hot, now, that's not necessary at all though. One second in the induction heater is enough, if I put it in my glass of iso it boils.

That's not even the main problem, the problem is waiting for it to be cool enough to not kill my transfer, who has time to wait several minutes for a tool that in the end just make a punch and I would have to do the actual transfer with my scalpel anyway :waits:

I have a FFU and don't really need to worry about leaving my scalpel for a however long I want, but in a SAB as soon as the punch or blade is cold contamination can land on it and the transfer is fucked :uhoh:

I do agree that a perfect round transfer looks very nice and if it was for a agar picture  competition I could see why one would want to do it, but for everything else an agar punch can

:begone:


I totally hear you on this - and I'm definitely not married to using a punch. A mutual buddy here on shroomery (who's a way better mush guy) suggested I do it because it helps to see uniform growth.

He's also a lot more skilled in this area than I am - in truth I had given up on the punches for the reasons I listed. The first time I did it I ended up dunking the punch in alcohol (I know, I know!).

In fact I'm pretty damn sure I killed my transfers onto 4 plates yesterday, and I'm really annoyed with myself over it. Although the tool was not red hot when I used it, surely it was still too hot for the myc. I don't see any growth 2 days later, and usually with a transfer you start to see SOME fuzz...

In any case, I value your thoughts @kloakk, and I thank you for always being so generous with your knowledge and ideas. It's always a pleasure to learn from you.

I'll probably go back to using a scalpel after this, or even using a needle as @Cicadoidea suggested.

I'm going to work on doing very very small transfers as well, on the idea that the smaller the transfer the less the likelihood of transferring contam along with it.

Also I've experimented with CS. I have absolutely butured some plates, even the agar roofs not staying in place, but still the myc has grown,. I think my problem is trying to do 4 of them on one plate like the video in another post showed.

I do have some small glass coverslips, so I'll probably PC them when I do another round of agar. I'll individually wrap the coverslips so they should stay sterile.

Thanks again everyone; you've all just confirmed to me that NOT using a punch is probably for the best! At least for someone with my limited skillsets.


DV8DUG said:
The solution to your problem is an easy fix. Be patient and let it cool down a bit.

Solved.


Just saw your message - I'm using an SAB. Where to put the tool for 1-2 minutes or something where it won't get contam? Sorry if I'm being dense...


--------------------
Mycelium? It's not MYcelium! It's YOURcelium!

Edited by Mychol (11/13/26 04:11 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mychol] * 1
    #29726385 -

Gonna throw in with the advice that punches are mostly fancy and inconvenient nonsense. You can cut smaller samples with a scalpel anyway, which is always ideal.

The one time I find it useful if for making numerous sequential punches on the same clean plate for transfer to a Castellani vial or for making up a bunch of smash wedges for trade/giveaway.

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Re: Ask Quick Questions, Get Quick Answers [Re: Marxcelium]
    #29726391 -

Thank you for the advice.

That bad huh?

I am nervous about using a credit card to purchase clones/spores over the internet. Aside from posting here with a VPN and securish browser this is the only thing tying me to an illegal activity.

My plan is to finish out what I have started, I have two bags now that are about 70% 30% colonized and they smell like how healthy bags are described.

But now I have gone to far and am already considering obtaining some liquid cultures from the forum sponsors. So in the end I might just take your advice and start over.


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I like cryptography and digital privacy

You can use PGP to pm me if you would like

Edited by hunter465 (11/13/26 04:41 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29726394 -

I am shopping around for some tools for agar plate work.

I had some individually sealed scalpels that I have been using but would like to get something I can sterilize over and over, along with some tweezers.

Anyone have any advice as to what I should get?


--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

Edited by hunter465 (11/13/26 04:32 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29726403 -

One other question

If I have 4.5lb bags of spawn how much substrate should I need preferably in grams or lbs if possible, can't quite wrap my head around qt's just yet.

If I am following the 1:2 ratio that would be roughly 10lbs of substrate but how much is that in dry ingredients like coir?


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I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29726405 -

I gotta say zit popping tools are great! Something Like these, sorry to link to a mega corp

In the beginning I wrapped each one individually in foil, but now I just use pouches. A bit wasteful I suppose, but 200 has last me over 2 years so far. I put three, so I have batches of sterile tools always available. but if I knew I was about to do agar, no reason you can't just sterilize more in one go.  sterilizing pouches.

What's great about them, is that they have two ends, so I use one side while being careful for one plate, and flip it if I am sure it didn't touch anything.

The kit I sent you has tweezers as well, which are IMO the best to get clone samples from inside of the stem. Hell, sometimes I use them to just grab a specifically rhizo looking cable.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29726406 -

I got a scalpel with replaceable tips from the art store. I mostly use it for stencils, but it is a tool that has many uses. Maybe check out your local equivalent?

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29726408 -

Thanks for the links, I will pick some up!


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I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Ask Quick Questions, Get Quick Answers [Re: sozu]
    #29726409 -

I do have an xacto knife with a bunch of replaceable blades, I guess I could just use that and replace the blades and sterilize everything.


--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465] * 1
    #29726570 -

Huskies is right with the zit poppers. Fungi perfecti sells a solid spear scalpel that doesn't have removable blades


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29726622 -

Just cut a wedge :crankey:


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29726632 -

How long would it take to sterilize 500ml agar at 20PSI, 259F

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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