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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29725475 -

Have another question.

How do you label your agar plates, I have seen prefix of t, and a few other things without any explanation.

For instance I have an original agar plate which has then been transferred 3 times so far to new plates.

I made a plate with an LC sample then transferred a sample from that plate to another plate, how should that be labeled?


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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465] * 2
    #29725500 -

Yeah, anymore than 2 shakes is excess. Shake once at 30% is all you need. If you shake too many times it can stress the grow out. Whether it results in a total stall or compromised state depends on a number of variables.

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge] * 1
    #29725510 -

Agar Wedge said:
Yeah, anymore than 2 shakes is excess. Shake once at 30% is all you need. If you shake too many times it can stress the grow out. Whether it results in a total stall or compromised state depends on a number of variables.


And once again thank you for a good answer.


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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29725516 -

hunter465 said:
I took a sample from my LC and squirted it onto a plate, and within 6 days the mycelium had taken over the plate. The sample was squirted all over the plate though.



6 days to fill a plate sounds like mold.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465] * 1
    #29725518 -

hunter465 said:
Have another question.

How do you label your agar plates, I have seen prefix of t, and a few other things without any explanation.

For instance I have an original agar plate which has then been transferred 3 times so far to new plates.

I made a plate with an LC sample then transferred a sample from that plate to another plate, how should that be labeled?


If it's an initial plate with a specific culture, I like to label them T0, germ plates I label "germ", growth transfered from a spot on a germ plate I'll label as T0, if taking multiple transfers from different points of germination on a germ plate I'll use a number before the T# . So say I streaked a plate with GT spores, that gets labeled germ, three spots start growing and I grab wedges from each, those are now GT 1 T0, GT 2 T0, and GT 3 T0. Transfers from that point on just get successive numbering, T1-T2 etc.


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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge]
    #29725523 -

So much appreciation - I will give those teks a try!

But just wanted to check, I see it says that bacteria cannot travel, but how about mould? Because I think if I have contam, it’s likely mould since it’s all white. And my understanding is that mould is also mycelium. So will an island or cabin still clean it up if it’s mould?

With making my own agar plates, I have most of the stuff arriving today. However, I note that I need a PC, but I have an induction stove top and I don’t see PC options available for that in my location. Electric PC seems like a possible alternative. Is 6L too small?


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See no evil, hear no evil 🙏

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29725532 -

your intuition about mold is right, way harder to clean AFAIK. cabins etc are for bacteria.

there definitely are induction stove pc's,  there's a steel bottom Presto, not sure about your area.

6l is real small. doesn't seem worth it to me

:cheers:


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Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29725540 -

DV8DUG said:
hunter465 said:
I took a sample from my LC and squirted it onto a plate, and within 6 days the mycelium had taken over the plate. The sample was squirted all over the plate though.



6 days to fill a plate sounds like mold.


Here are some photos in this thread of my plates, let me know what you think

https://www.shroomery.org/forums/showflat.php/Number/29724718


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Re: Ask Quick Questions, Get Quick Answers [Re: fontinalis666]
    #29725541 -

Thank you very much


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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood] * 1
    #29725546 -

Happy to help!:smile::mushroom2:

Yeah, the PE culture would be a tough one to clean up because of the mold.

The GT looks good though! Clean that up a little and you'll be cranking out lots of mushrooms in no time:rockon:

For an induction capable canner check out the presto 23 qt (01784)
I use a 12qt presto precise electric canner that works great. Holds 5 quarts at a time.  Which is half of what the 23qt holds, but that's okay with me

There's also the option of using a instant pot if you'd like. Here's a Tek for growing with a Instant pot

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood] * 2
    #29725549 -

CeeGood said:
So much appreciation - I will give those teks a try!

But just wanted to check, I see it says that bacteria cannot travel, but how about mould? Because I think if I have contam, it’s likely mould since it’s all white. And my understanding is that mould is also mycelium. So will an island or cabin still clean it up if it’s mould?

With making my own agar plates, I have most of the stuff arriving today. However, I note that I need a PC, but I have an induction stove top and I don’t see PC options available for that in my location. Electric PC seems like a possible alternative. Is 6L too small?



They also sell induction plate adapters that can go on the bottom of any pot and work with your stove top.


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29725567 -

I have likr 1/8 of an inch of something around myc on plate. It's almost transparent, you can't really see it until you put it to light.
Here are pics:



One plate that I did on water agar is whole like that and other one that's cabin(very low nute) is the same.
Is that mold? Any threads on cleaning it?


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I certainly hope we will
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Re: Ask Quick Questions, Get Quick Answers [Re: Reishi Uchiha] * 2
    #29725634 -

I think it's just thin myc. If you think it's mold try to pour a layer of hot agar over top


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Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29725766 -

I had a false alarm for LEucistic KSSS earlier. But, this time perhaps not.



Whatcha think, just very pale cap, albino or Leucisitic?


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Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29725785 -

Just put some 7 year old MSS to some agar.
I ended up putting a few drops instead of one since its so old.

I know the extra moisture isn't good, will it ruin it though?


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Yahoo deleted my email from inactivity that I use to use to access my old account from back in 2018.
-Until I can get that sorted out im back on a new account. (Yuck)

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg] * 2
    #29725792 -

It'll be fine.


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Re: Ask Quick Questions, Get Quick Answers [Re: hunter465]
    #29725816 -

hunter465 said:
DV8DUG said:
hunter465 said:
I took a sample from my LC and squirted it onto a plate, and within 6 days the mycelium had taken over the plate. The sample was squirted all over the plate though.



6 days to fill a plate sounds like mold.


Here are some photos in this thread of my plates, let me know what you think

https://www.shroomery.org/forums/showflat.php/Number/29724718


I think you should find an alternate source for spores and/or clones and start over.

You don't need azomite and gypsum. Vermiculite only when you over hydrate your coir.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29726091 -

sozu said:
I have already got the Petri dishes. Fun story, they were out of the size that I found recommended by the community so they sent me the 10cm variant for no fee. So now i should get some agar and get started with cloning. Which of the fruits should i go with? Roger suggests going with the ones that are clustered, but what is the modern understanding?

picunrel: kind of


Some basic attributes you’d want to look for are size, speed, clustering, and solid stipes

Have you read the hitchhikers guide yet?


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𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29726099 -

The first three items I recognize. But what means solid stipes? This is unfamiliar.
Also: your reference to the hitchhikers guide: I will figure this out.

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Re: Ask Quick Questions, Get Quick Answers [Re: sozu]
    #29726100 -

The stem of the mushrooms official name is stipe.

Hitch hikers guide is good.
Here's a link to it.

https://www.shroomery.org/forums/showflat.php/Number/28501533#28501533


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Edited by Mystikal (11/13/26 07:31 AM)

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Shop: Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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