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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Progress so far * 2
    #29724718 -

Introduction

Hello everyone. I have been sucking up as much info from this forum as I can, and I would like to get some feed back on how I am doing so far, and what I plan to do in the next few weeks. Let me know if what I have done, and plan to do are good or bad.

What I have done so far

Culture

- Obtained a sample of P. Cubensis from buying some dried mushrooms from a random person. Got the samples from the inside of the stem under a isopropyl alcohol sterilized SAB.
- Placed samples into a few different agar plates using a SAB.
- Once the samples looked good, and I could not detect any bacteria or mold I moved a small section from the agar to another few fresh plates.
- Let that grow for a bit.
- Then under a SAB and using PC sterilized LC jars made some LC.
- Waited a few weeks for LC to mature.
- Once the LC matured I withdrew a sample of about less than 1ML of liquid and put it on a new agar plate all under a SAB.
- Checked that new plate once it matured for any infections, was happy with it and decided it was time to inoculate.

Bags
- Soaked 4.5lbs of whole oats in one 5 gallon bucket for about 10 hours.
- Soaked 4.5lbs of birdseed in another 5 gallon bucket for about 10 hours.
- Rinsed everything.
- Then simmered both on the stove for about 10 - 20 minutes.
- Checked for hydration of oats by cutting some open, was satisfied.
- Let dry for a bit, and helped along using a hair dryer.
- Used the paper towel method to test dryness.
- Once properly dry, moved 2.25lbs of whole oats and 2.25lbs of birdseed to autoclave bag with a breather and injectable port. (Next time I will not use bird seed because a lot of the grain split open).
- Sterilized 2 4.5lbs of the whole oats and bird seed mix separately. I evacuated the PC for about 10-15 minutes for each sterilization cycle, to get rid of all air in the PC. Ran the PC for 3 hours each for both cycles at as close to 15psi as I could, I live somewhere with high altitude so I adjusted my PC time to account for this.
- Folded the bags in such a way so there was a plenum before sterilization.
- When pulling the bags out of the PC I transferred them quickly to a isopropyl alcohol sterilized SAB.
- Slightly unfolded the bags as to not lose the plenum and heat sealed the bags with a heat sealer.
- Then in that same SAB I injected the bags.
- So far its been about a week since I injected the bags
- The bags have some good mycelium growing already all along the bottom and they smell like cooked oats and a little earthy like mushrooms.

Going Forward

Tub Setup
- I have two bags of 4.5lb grain/bird seed spawn, I plan to birth them in two separate 66qt Sterlite tubs.
- The tubs have 2" holes drilled in 6 spots on the tub. Two each on the long side of the tub 4-5" above the bottom of tub, and one on each short side of the tub close to the lid. Each hole currently has a 2.5" filter disc.
- I do not plan on taping the holes on the inside to separate spawning and fruiting, will just treat it as spawning the moment I put the CVG and grain spawn mixture inside.

CVG
- I plan to mix 10lbs - (850g Coir / 3.6 Liters water) with some azomite and gypsum, maybe a little bit of vermiculite into a 5gal bucket. This is per 4.5lb grain spawn bag, so I plan to make two batches of CVG.
- Boil the water and place on the coir, mix up, test water retention, and let sit over night.
- Next day reserve about 1qt of the CVG mixture for top soil and mix the rest with the grain spawn and spread in the 66qt tubs.
- I will use a tile float to spread the mix evenly and compact it slightly using that tile float.
- Then spread that reserved 1qt of CVG on the top of the mixture and slightly compact that as well.
- Hoping the compaction will not require me to use a liner.

Wait and Hope
- Then from there I will basically just check conditions in the box and look for humidity. Water pooling means more FAE, not enough humidity means I will have to mist.
- Once the veil has broken I will harvest the crop using the method where you fill the tub with water and use a knife to cleanly cut all the mushrooms from their stems.
- Then hopefully get a flush or two more.

Drying
- Once I have harvested I will put everything in my dehydrator with some silica packs and crank up to max temp and let sit for 24 hours.
- Then store the mushrooms in large ziplocks with those cooked silica packs.

Thanks for reading

It was a little long winded, but if you read it and think I did something wrong, or plan to do something wrong, please let me know.

Thank you to everyone who has posted in these forums over time, the information has proved invaluable.

Also I tried using an AIO bag back when I was in highschool, and injected them with straight spores. Everything got contaminated, and I had to abandon the whole secret grow before my parents found out. Now I am back again, hope things go better this time.

Thank you.

Edited by hunter465 (11/11/26 06:59 PM)

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Re: Progress so far [Re: hunter465] * 1
    #29724720 -

I am curious to see what those plates with the dried clone look like. Such things are not impossible however most would consider the degree of difficulty to be not worth the effort. If the mushrooms were dried with heat it is likely that the tissue is denatured and will not revive.


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Re: Progress so far [Re: Pastywhyte]
    #29724724 -

Give me a moment and I will post some metadata stripped photos.

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Re: Progress so far [Re: Pastywhyte]
    #29724725 -

Here is the original result of the biopsy. This was all done in a SAB to eliminate any outside contamination. It's possible that it is contaminated regardless to my precautions because I am still new to recognizing mycelium vs contam. So please be kind. You can see the little pieces of the stem still on the plate and thats where the growth is coming from.







Edited by hunter465 (11/11/26 06:06 PM)

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Re: Progress so far [Re: Pastywhyte]
    #29724731 -

Here is a plate where the LC was squirted on. This is second generation from the first photos I linked. So I allowed the first agar plate to take off, then took a healthy slice from there, put it on another gar plate, let that take off and then put that agar in the LC jar and then transferred about less than 1ML to this plate. All was done using a SAB.

I believe it is mycelium because:
- it's white
- fuzzy
- fine, threadlike structures like hyphae
- radial growth
- Some areas resemble tomentose mycelium by their cottony texture





Here is the LC jar. The big floating bits are the agar I cut up to put in the jar.



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Edited by hunter465 (11/11/26 06:22 PM)

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Re: Progress so far [Re: Pastywhyte]
    #29724752 -

Sorry to spam.

But when taking the biopsy from the stems of those mushrooms I had noticed that they were not 100% dry. When I cracked open the stem the insides seemed fluffy and cottony and a little moist.

Also I got the idea from a youtube video about cloning any and all mushrooms from dried material. I know that youtube is not a great source so after some digging I found a good source that backed up the idea this paper: https://strathprints.strath.ac.uk/96753/


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I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Progress so far [Re: hunter465]
    #29724823 -

Can you post in focus photos of your plate with light coming from above.


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🍄OFFICIAL SEMILANCEATA THREAD🍄



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Re: Progress so far [Re: Baba Yaga]
    #29724849 -

:whathesaid:

Man, you are sucking up information like a sponge. Excellent detail and operation, staying tuned for updates

:camping:

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Re: Progress so far [Re: Baba Yaga] * 1
    #29724858 -

Hope these pics are better, my camera is not great at focusing close up






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I like cryptography and digital privacy

You can use PGP to pm me if you would like

Edited by hunter465 (11/11/26 09:05 PM)

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Re: Progress so far [Re: hunter465]
    #29724874 -

If you are using a phone camera, look for an expert mode or similar that allows you to adjust all sorts of values that I have no idea about but the MF value lets you adjust the focus manually, very handy for close up shots where autofocus is all over the place :thumbup:


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Re: Progress so far [Re: hunter465]
    #29724876 -

It looks like there is indeed cube mycelium in there. However it still needs cleaning up. I'd suggest you employ one of following until you get something more organized looking.

Cabin

And or

Island


The LC germ plate shows signs of rhizomorphic growth, which is promising, however that bottom plate is clearly struggling with something, or a couple things. That long clearish section and disorganized growth towards the center is the give away. This being said, I wouldn't inject anything with the LC.

This is a great guide for identifying contamination on plates.

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Re: Progress so far [Re: Agar Wedge]
    #29724884 -

Thank you for the reply. The bottom photo is the original plate where I took a disection from to transfer to new plates. The middle part is a chunk missing.


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I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Progress so far [Re: Agar Wedge]
    #29724888 -

I will try out the cabin tomorrow or the islands tomorrow. Would like to get some pure agar plates


--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Progress so far [Re: hunter465]
    #29724893 -

Happy to help!

Stick with it, you will :wink::mushroom2:

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Re: Progress so far [Re: Turvenuija] * 2
    #29724895 -

Ok I believe this is the best I can do.





--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Progress so far [Re: hunter465] * 1
    #29724898 -

Rock on dude! When I read that you cloned dried mushrooms I was like..... what??  But then you said that they weren't all the way dry. 1. Yikes at whoever didn't dry them properly and 2. I'm glad for your sake that they weren't all the way dry because it looks like the universe wanted you to have some Mycelium to play with. Congratulations Hunter!


hunter465 said:
I will try out the cabin tomorrow or the islands tomorrow. Would like to get some pure agar plates


I just made a little write up for a low nutrient agar recipe and it contains Milkboy's PDA tek, Agar Wedge's Island tek, Marcus Mycelius' Trench Tek, and Siphonophorest's write up on Cabin Sequestering. Wedge already linked them above because he's freaking awesome. It's helped me clean up some nasty embedded bacteria / biofilm. I've found with vendor purchased Agar plates, their nutrient content tends to be on the high end which feeds both embedded contamination and mycelium quickly. With lower nutrient agar plates, the bacteria gets slowed down while the mycelium spreads quickly looking for more nutrients.

Congratulations bro, I'm excited to see how this turns out for you!


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Lo-Z Things
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Things that saved Lo-Z
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Edited by Lo-Z (11/11/26 09:59 PM)

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Re: Progress so far [Re: Lo-Z]
    #29724911 -

I was also a little shocked at how well the plates took off and that I got anything from those random mushrooms.

So I am still low tech on the agar plates, I just bought some off amazon because I have not dipped my toe into making my own yet. I guess I will have to do that as well.

Though I do still have some left over that I will use up to try out the cabin. I have decided to just go ahead and take a crack it this evening.


--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Progress so far [Re: Lo-Z]
    #29724918 -

For real though, sucks that guys selling fan dried shrooms :hehehe: but hey, hunters out here making the best of someone else's mistakes :rockon:

Here's a great Agar tek to help familiarize you:mushroom2:

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Re: Progress so far [Re: Agar Wedge] * 1
    #29724930 -

Ended up trying out the cabin method. Sorry for the potato quality photo but its the best I can do.



--------------------
I like cryptography and digital privacy

You can use PGP to pm me if you would like

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Re: Progress so far [Re: hunter465]
    #29724935 -

Right on man!:mushroom2: If the culture isnt as clean as it should be afterwards, you can also use sterilized microscope cover slips as a roof. It sits more plumb than an agar roof, therefore yields better results:awesomenod:

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Mono Tub Substrate   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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