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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Lo-Z's Lo-Nutrient Potato-Molasses Agar Recipe (LNPMA) * 3
    #29724759 -

I originally started writing this in my Journal, but I'm sure others can benefit from it too.

I use Milkboy's PDA tek, But I like things a little more precise than he writes it. Shout out to Milkboy for the original tek. This is my refined recipe based off of his tek.

French press is
:chefskiss:


Low-Nutrient Potato-Molasses Agar (0.45%)

Batch Size: 500 mL
Nutrient Concentration: ~0.45%
Agar Concentration: 2.5%


Ingredients

Distilled Water: 500 mL
Instant Potato Flakes: 0.75 g
Molasses: 1.50 g
Telephone Brand Agar Agar Powder: 12.50 g
- Total Added Nutrients: 2.25 g (0.45%)



Preparation

1. Measure 500 mL of distilled water.
2. Place in French press
3. Add 0.75 g of instant potato flakes to water.
4. Stir flakes for 30 seconds then let all the flakes fall to the bottom.
5. Use French press to strain flakes out of water, pour directly into pot.
6. Bring water to simmer.
7. Add 1.50 g of molasses.
8. Mix thoroughly until the ingredients are evenly dispersed.
9. Add 12.50 g of agar agar powder and mix thoroughly.
10. Transfer the mixture to media bottle, leaving sufficient headspace.
11. Sterilize at 15 PSI for approximately 20–25 minutes, following appropriate pressure-sterilization procedures.
12. Allow the medium to cool to approximately 45–50°C (113–122°F).
Personally I pour with it hot as f*ck still, just hot enough that its uncomfortable with thick cloves on.

13. Pour into sterile Petri dishes using aseptic/ sterile technique.
14. Leave closed but unwrapped in SAB with arm holes close until condensation is evaporated (possibly up to 5 days+).
15. Allow the plates to cool and solidify before use.


Nutrient Calculation

0.75 g Potato Flakes + 1.50 g Molasses = 2.25 g Total Nutrients
2.25 g ÷ 500 mL × 100 = 0.45%
Maximum Target (0.5%): 2.50 g

Actual Added Nutrients: 2.25 g
Below Maximum By: 0.25 g


Note: Nutrient percentage is calculated from the combined weight of potato flakes and molasses relative to the starting water volume. Actual available carbohydrate concentration may vary depending on ingredient composition.

The Purpose of Low Nutrient Agar:
Low-nutrient agar formulation is intended for mycelium isolation and culture cleanup. Reduced nutrient availability may slow bacterial growth, potentially allowing healthy mycelium to extend beyond contaminated areas for transfer to fresh agar.
Low-nutrient conditions do not guarantee that mycelium will outgrow bacterial contaminants.

Using Lo-Nute agar in combination with Islands, Trenches, and Cabins is highly recomended.







First Plate Purchased from Vendor | 2nd plate LNPMA Trench Transfer | 3rd plate LNPMA CS Transfer

Edited by Lo-Z (11/11/26 08:21 PM)

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Re: Lo-Z's Lo-Nutrient Potato-Molasses Agar Recipe (LNPMA) [Re: Lo-Z] * 1
    #29724776 -

Nice! I have some molasses and instant potatoes, and I need to make more agar, I'll try this one out!


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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