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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood] * 1
    #29723398 -

CeeGood said:
Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?


🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723403 -

CeeGood said:
Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?


TEAM GLASS PETRI


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

Edited by langten69 (11/10/26 01:20 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge]
    #29723482 -

Thanks for responses RE: Petri… seems like it’s personal preference. The ones I have that are pre-poured are plastic, but I’m having trouble with condensation and not being able to see my specimens clearly. I thought perhaps glass may help with that or is it the same?

Have some really crappy photos, due to poor lighting and condensation.

This is sample one - GT’s from spore syringe, 6 days post. Used 10ml in grow bag, minus drops for agar. Was concerned about contam on this plate, but everything is white so far?





Sample 2 - PE from what I think was liquid culture, only used 5ml in grow bag now 6 days ago. Agar had presentation on day 2. I see some powdery white in grain but looks slower than the GT MSS grow bag… maybe because I only used 5ml?





Someone mentioned I might have a bacterial problem in my grow bags… would love to know what might indicate this? I know in sample 1, grain looks a little wet, but it’s not IRL.

Praying that everything is ok. I inoculated another two plates yesterday hoping to get some better cultures.


--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

Edited by CeeGood (11/10/26 03:46 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723495 -

I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


--------------------


.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III HAPPENING NOW :salute:

🧪 Castellani Preservation | 🔬 The Slidewich | ☠️ Touchless skull sterilizer | 📸 Agar plate scanner


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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723497 -

Glass plates are usually slightly more transparent

To get rid of condensation you can put something warm on the lid to evaporate the condensation and then store the plates upside down

GT plate looks good, but the PE plate is too blurry for me to tell

I cant tell if in the GT grain bag pic is either really rhizo mycelium or the reflection of a light

The PE bag is too early to tell but powdery is usually a word that describes mold so I hope it's not mold

:prayer:

GL with your grows


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius]
    #29723498 -

Rasmodius said:
I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


PCing sounds excessive...

But I suppose (if you wanna try hard) you could cut individual cap sized foil squares and stack them in the hundreds then wrap those inside of more foil and PC like 500 - 1000 squares at a time


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

Edited by langten69 (11/10/26 04:54 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius] * 1
    #29723501 -

Rasmodius said:
I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


I cut individual sheets from the roll, wipe with iso and let it evaporate in the tub I'm going to print in, when I'm done printing I wrap them in the flow. It has been good to me thus far. The caps ain't exactly sterile going on the sheets, but printing the cap a second time might give a cleaner print.

That said, The Tao make sheets and bake them in the oven, don't remember the details but maybe he'll see this and chime in.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: langten69]
    #29723525 -

Really appreciate your answer thank you! Who knew cling film was semipermeable - learn something new every day in this hobby!


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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood] * 1
    #29723532 -

The gt plate is normal mycelium, the PE looks like mold, the mycelium should not be translucent like that :mushroom2:

You don't need to shell out for pyrex, any borosilicate glass will do

With the grain I wouldn't worry so much yet. Those tan dots look like the debris at the bottom of my millet bucket. I'm guessing it was just the end of the container the grain was in.

Thanks for sharing! Will stay tuned for updates :camping:

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29723586 -

Kloakk said:
Rasmodius said:
I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets?
....


That said, The Tao make sheets and bake them in the oven, don't remember the details but maybe he'll see this and chime in.


I don't remember if it was The Tao specifically, but... I've seen someone cut out the sheets to lay on a rimmed baking sheet, covered the whole thing in foil, baked that in the oven, and put the cooled tray in a SAB before removing the top foil.


--------------------
Feeling lost here? We get it. Here's the most up-to-date guide as of 2026.


I know I'm a fool, but that's why I keep trying to learn.
:peace::heart::mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius]
    #29723591 -

Rasmodius said:
I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


Here it says foil comes sterile as you unwrap it. Never done it yet, just remember reading this.


--------------------
Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29723606 -

Huskies said:
I used Pasty's older school micropore tubs, but recent ones I swapped to his updated Ez Dial, which is yeah, just a few holes. Been working great with my 6qt shoeboxes and 20L tubs.

https://www.shroomery.org/forums/showflat.php/Number/26140339/page/1

It's not that the older methods don't work, they do, but this is a hassle free one, don't have to tape it up, replace tape.

I haven't noticed contamination issues, reality is once its spawned the grain is clean or it isn't, ambient spores aren't going to be the deciding factor.

Only thing that these could be worse with are if you have a gnat problem, since they can enter relatively easily.



Im very interested in the newer EZdial I just have some nasty general anxiety and it makes me want to ask 100 questions before im comfortable with re starting.

For someone restarting fresh, not knowing the spore load in my house or how the new area ill be leaving the tubs in will affect micro climate in the monos should I be safe and restart with the micropore tape method and move up to the EZ dial when I get familiar with my new area?

Sorry for the late and long reply and excuse my ignorance ive been gone a long time lol

I miss MudaFuka, I could always PM him with my questions 😔


--------------------
Yahoo deleted my email from inactivity that I use to use to access my old account from back in 2018.
-Until I can get that sorted out im back on a new account. (Yuck)

:migraine:

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg]
    #29723618 -

Curious if many cultivators have used Peatmoss outside of the amazing job it does with casing?

Historically I used it for a peatbog substrate for Tampanesis fruiting, and sometimes blended in for CVPG.  Did some tests years ago on Natalensis (seems it's Ochra now) and was good at 4" or so without issue

Running mainly exotics rn, and considering some tubs of PVG (pH adjusted) to 7.5-8 ish

It's price and consistency for product quality/sourcing is attractive, plus it hold more than coir, but could be potentially less attractive (heavy?) for some species that need high aeration / fluffy myc

Current projects:

Pan Cyan Eungai
EU x Komodo
Pan Bisporus
Ochra
Tampa
Tampa var ATL7


Thx 🙏


--------------------
Link to my journal thread

https://www.shroomery.org/forums/showflat.php/Number/29701994

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg] * 1
    #29723620 -

this sums it up


--------------------
Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg] * 1
    #29723630 -

Twigg said:
Huskies said:
I used Pasty's older school micropore tubs, but recent ones I swapped to his updated Ez Dial, which is yeah, just a few holes. Been working great with my 6qt shoeboxes and 20L tubs.

https://www.shroomery.org/forums/showflat.php/Number/26140339/page/1

It's not that the older methods don't work, they do, but this is a hassle free one, don't have to tape it up, replace tape.

I haven't noticed contamination issues, reality is once its spawned the grain is clean or it isn't, ambient spores aren't going to be the deciding factor.

Only thing that these could be worse with are if you have a gnat problem, since they can enter relatively easily.



Im very interested in the newer EZdial I just have some nasty general anxiety and it makes me want to ask 100 questions before im comfortable with re starting.

For someone restarting fresh, not knowing the spore load in my house or how the new area ill be leaving the tubs in will affect micro climate in the monos should I be safe and restart with the micropore tape method and move up to the EZ dial when I get familiar with my new area?

Sorry for the late and long reply and excuse my ignorance ive been gone a long time lol

I miss MudaFuka, I could always PM him with my questions 😔


No worries man.

Honestly, I think you are safe. Once again, it's not really ambient spores that cause contamination, it's grains getting contaminated as it colonizes. A tiny bit can ruin an entire tub, but geniunely clean spawn doesn't contaminate from ambient spore loads, at least that is the consensus here.

I have an air purifier that I sometimes run in my grow room. Nice to see a 100% air quality, but even that doesn't mean no particulates or mold spore. If it helps your anxiety, could be worth looking into, but maybe you just need a success to go again.

If you are going to be making new tubs, you can try one in each method. Both work, you can see what works better in your place.

But the EZ Dial is not a risky thing, but rather a group consensus great option. I use my blow torch to heat up a thin, small designated screwdriver, and use that to make the holes. BEtter than drilling cause it won't cause breakage, but I'd do it outside or close to an open window due to melted plastic fumes.

Your spawn prep and inoculation is where contamination is more likely, so feel free to let us know what your plan is.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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