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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723398 -

CeeGood said:
Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?


🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723403 -

CeeGood said:
Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?


TEAM GLASS PETRI


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

Edited by langten69 (11/10/26 01:20 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge]
    #29723482 -

Thanks for responses RE: Petri… seems like it’s personal preference. The ones I have that are pre-poured are plastic, but I’m having trouble with condensation and not being able to see my specimens clearly. I thought perhaps glass may help with that or is it the same?

Have some really crappy photos, due to poor lighting and condensation.

This is sample one - GT’s from spore syringe, 6 days post. Used 10ml in grow bag, minus drops for agar. Was concerned about contam on this plate, but everything is white so far?





Sample 2 - PE from what I think was liquid culture, only used 5ml in grow bag now 6 days ago. Agar had presentation on day 2. I see some powdery white in grain but looks slower than the GT MSS grow bag… maybe because I only used 5ml?





Someone mentioned I might have a bacterial problem in my grow bags… would love to know what might indicate this? I know in sample 1, grain looks a little wet, but it’s not IRL.

Praying that everything is ok. I inoculated another two plates yesterday hoping to get some better cultures.


--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

Edited by CeeGood (11/10/26 03:46 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723495 -

I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


--------------------


.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III HAPPENING NOW :salute:

🧪 Castellani Preservation | 🔬 The Slidewich | ☠️ Touchless skull sterilizer | 📸 Agar plate scanner


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Re: Ask Quick Questions, Get Quick Answers [Re: CeeGood]
    #29723497 -

Glass plates are usually slightly more transparent

To get rid of condensation you can put something warm on the lid to evaporate the condensation and then store the plates upside down

GT plate looks good, but the PE plate is too blurry for me to tell

I cant tell if in the GT grain bag pic is either really rhizo mycelium or the reflection of a light

The PE bag is too early to tell but powdery is usually a word that describes mold so I hope it's not mold

:prayer:

GL with your grows


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius]
    #29723498 -

Rasmodius said:
I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


PCing sounds excessive...

But I suppose (if you wanna try hard) you could cut individual cap sized foil squares and stack them in the hundreds then wrap those inside of more foil and PC like 500 - 1000 squares at a time


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

Edited by langten69 (11/10/26 04:54 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius]
    #29723501 -

Rasmodius said:
I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....


I cut individual sheets from the roll, wipe with iso and let it evaporate in the tub I'm going to print in, when I'm done printing I wrap them in the flow. It has been good to me thus far. The caps ain't exactly sterile going on the sheets, but printing the cap a second time might give a cleaner print.

That said, The Tao make sheets and bake them in the oven, don't remember the details but maybe he'll see this and chime in.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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