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DV8DUG
Hyphal Exhibitionist

Registered: 12/27/23
Posts: 2,297
Loc: Melniboné
Last seen: 2 hours, 32 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: CeeGood]
#29723398 - 11/10/26 01:09 AM (3 hours, 9 minutes ago)

CeeGood said:

Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?

🅃🄴🄰🄼 🄲🄾🄽🄳🄸🄼🄴🄽🅃 🄲🅄🄿

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Agar Plates and Removing Hidden Contaminants - A Visual NotebookRecognizing Contamination in Grain Jars - A Visual NotebookContaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation🅃🄴🄰🄼 🄲🄾🄽🄳🄸🄼🄴🄽🅃 🄲🅄🄿

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langten69
🥋 Contam Eradicator : PE Guru

Registered: 08/18/26
Posts: 1,491
Loc: 'Merica
Last seen: 43 minutes, 16 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: CeeGood]
#29723403 - 11/10/26 01:15 AM (3 hours, 4 minutes ago)

CeeGood said:

Hi, I’m super new and have recently just inoculated my first grow bags and agar. Is there a trick to getting really thick healthy mycelium on agar plates? Mine looks really thin… should I put it on heat or something?

Also I purchased pre-poured plates, but I’d like to learn to pour my own. Are glass or plastic disposable petri’s more preferable?

TEAM GLASS PETRI

--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG : H.D. : PLASTIC-MYC : TGE : JOIN CAKE WARS : MODERN FRUITING : MCWA : QDMT : ♾️ : MY FAV TEKS 📖

Edited by langten69 (11/10/26 01:20 AM)

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CeeGood
Spore Explorer

Registered: 11/07/26
Posts: 6
Last seen: 33 minutes, 11 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: Agar Wedge]
#29723482 - 11/10/26 03:44 AM (35 minutes, 34 seconds ago)

Thanks for responses RE: Petri… seems like it’s personal preference. The ones I have that are pre-poured are plastic, but I’m having trouble with condensation and not being able to see my specimens clearly. I thought perhaps glass may help with that or is it the same?

Have some really crappy photos, due to poor lighting and condensation.

This is sample one - GT’s from spore syringe, 6 days post. Used 10ml in grow bag, minus drops for agar. Was concerned about contam on this plate, but everything is white so far?

Sample 2 - PE from what I think was liquid culture, only used 5ml in grow bag now 6 days ago. Agar had presentation on day 2. I see some powdery white in grain but looks slower than the GT MSS grow bag… maybe because I only used 5ml?

Someone mentioned I might have a bacterial problem in my grow bags… would love to know what might indicate this? I know in sample 1, grain looks a little wet, but it’s not IRL.

Praying that everything is ok. I inoculated another two plates yesterday hoping to get some better cultures.

--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

Edited by CeeGood (11/10/26 03:46 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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