Reishi Uchiha said:

Muffa said:

I wouldn't reuse the plates, but if you are low on them, I feel you and understand why you're thinking about it.
In case it's a "desperate" situation, reuse them, but transfer as soon as possible and keep everything as far away as possible from the other transfers.

Regarding the size of transfer, it can work out even with super tiny pieces of tissues, that's why things like Josex's poke work. But in my experience bigger wedge transfers catch up better, and when doing super tiny aerial myc transfers, sometimes there are no signs of lifeat all.
Do you have pictures of the plate you transfered from?

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Hey, I appreciate your input!
No, I don't have any pics, I use pp5 screw on and there is still condensation that would prevent a good quality photo. The thing is that at the same time I made a transfer of a same or slightly smaller size to the cabin which is showing growth, maybe even a day after I saw some tiny hyphae.
But these on water agar have no growth at all, like I've put flashlight from all the angles, and nothing, not even on top of those wedges. Hence why the question.
I was trying to make sure there is some use of them before I toss them to make new ones. And yes I'm low on em. I've only bought 20 of those since that's all I could afford so I gotta juggle with them now cuz I've started a bit too many cultures

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Tri said:

QQ
Any of the more science brained among us have book (or anything else) recommendations for getting into the finer details of this hobby?

I know any mycology textbook would do good but if there’s some S tier learning materials I’d rather be as efficient as possible

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Wall.E said:

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Good to see you.

langten69 said:

Tri said:

QQ
Any of the more science brained among us have book (or anything else) recommendations for getting into the finer details of this hobby?

I know any mycology textbook would do good but if there’s some S tier learning materials I’d rather be as efficient as possible

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What would you consider as the finer details?

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Nichrome said:

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venetian-cubes said:

For unmodded lids on grain spawn (not pf tek)

After inoculation, do you put foil back on?

And, how tight should the rim be?

I have researched it and found conflicting responses so wanted to see if there was any logic I could follow.

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Nichrome said:

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auroradawn said:

So just to confirm something because I believe I have been an idiot: Instapots need to be vented too?

Because my dumb ass has been thinking I didn't need to because the IP will do that as part of its cycle. But now I'm realizing that makes no sense because if I leave the valve closed how would it be venting 🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️

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auroradawn said:

So just to confirm something because I believe I have been an idiot: Instapots need to be vented too?

Because my dumb ass has been thinking I didn't need to because the IP will do that as part of its cycle. But now I'm realizing that makes no sense because if I leave the valve closed how would it be venting 🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️

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Kloakk said:

auroradawn said:

So just to confirm something because I believe I have been an idiot: Instapots need to be vented too?

Because my dumb ass has been thinking I didn't need to because the IP will do that as part of its cycle. But now I'm realizing that makes no sense because if I leave the valve closed how would it be venting 🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️

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In theory the instant pot vents on it's own by design and should be set to sealing as soon as you start. It should vent all air pockets out before it seals completely.

What are you trying to sterilize?

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Kloakk said:

auroradawn said:

So just to confirm something because I believe I have been an idiot: Instapots need to be vented too?

Because my dumb ass has been thinking I didn't need to because the IP will do that as part of its cycle. But now I'm realizing that makes no sense because if I leave the valve closed how would it be venting 🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️🤦🏻‍♀️

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In theory the instant pot vents on it's own by design and should be set to sealing as soon as you start. It should vent all air pockets out before it seals completely.

What are you trying to sterilize?

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The Tao said:

I vent my instant pot for 10 minutes. What is your process?

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