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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: North Spore Bulk Substrate, Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29660107 -

Pastywhyte said:
Okay forgive my boomerness. I had no idea pouring condiment cups was common practice now. That shit is sus af to me, I would not even consider that a viable method. Are they at least sterilizing them beforehand?

Apologies again, I was out of my depth. Crazy how things change.


I've never used condiment cups myself, but I know DV8DUG have used them forever and his agar game is as solid as can be. Besides it must be at least as sterile as cling wrap and people use that all the time.


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk]
    #29660114 -

Cling wrap goes outside the plate tho. If it works consistently for some then I can't knock it. But it seems crazy to me.


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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29660128 -

Pastywhyte said:
Cling wrap goes outside the plate tho. If it works consistently for some then I can't knock it. But it seems crazy to me.


It's takes heat to manufacture them and I'm sure it's made under pretty clean conditions, then comes the hot agar mix. I have never had a poured, unused plate get contaminated.

Then there's the smashed wedge where a piece of colonized agar is sent in a zipbag. I have received plenty and only once have I had a problem, which could be my own fault, point being it seems plastic is pretty sterile.

I do agree with you though and is why I use disposable plates and wrap with parafilm :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk] * 1
    #29660173 -

Ziplocs are def sterile inside and I've used them in lieu of wrapping plates. I can dig it. Though I also kinda dislike smashed agar on general principles. That's just more boomer talk.


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:headbang3: Pastywhyte's Easy Writeups :headbang3: My Favorite Grow Logs :headbang3:
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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29660257 -

Pastywhyte said:
Ziplocs are def sterile inside and I've used them in lieu of wrapping plates. I can dig it. Though I also kinda dislike smashed agar on general principles. That's just more boomer talk.


:lol: Boomer or not I'm right there with you. I like that with a wedge you can get a great culture straight out the bag, but I would take a dried grain over smashed wedge any day :awesomenod:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk] * 3
    #29660301 -

And I have never wrapped a plate in my life. Or even own one...


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Is this contam? LC and agar [Re: DV8DUG]
    #29660303 -

DV8DUG said:
And I have never wrapped a plate in my life. Or even own one...


I don't see any reason to change something that is working perfectly fine so why would you :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: DV8DUG]
    #29660396 -

DV8DUG said:
And I have never wrapped a plate in my life. Or even own one...


TBH I totally get this, is why I was big into no pour for so long. If it works it works.


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:headbang3: Pastywhyte's Easy Writeups :headbang3: My Favorite Grow Logs :headbang3:
:headbang3: Avoiding the dreaded facepalm in cultivation :headbang3: The worst threads all have the same subjects :headbang3:
:headbang3: Q & A.M.U. :headbang3:

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Re: Is this contam? LC and agar [Re: Thought Criminal] * 1
    #29661561 -

@Thought Criminal, we haven't seen you much since you started this thread. I just wanted to say don't get discouraged and don't give up.

Getting contamination early on is disappointing and a setback, but a minor setback that can easily be corrected by tightening up your sterile technique and sterilization habits. Besides reading and advice, the best way to get on track is from practice and developing good habits. You will get the hang of it in no time, I'm sure.

Keep in mind all of us have had contamination. It is normal and to be expected. Also not a big deal.

Also you can keep the LC on the back burner, at least until you get your agar game down pat. Quite honestly you can live without doing any LC at all.

Get back on the horse and practice. You'll be a pro before you know it. :thumbup:


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The Combo Tek - Easy grow method for newbies and pros alike

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Re: Is this contam? LC and agar [Re: veggie]
    #29661574 -

:whathesaid:

Its just water under the bridge
Its good experience under your belt
You got this


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When my pies have nine lives?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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