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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #29634854 -

Hey man i know this thread is old, I was just curious how much LME you'd add for the 300ml jars, i know you said you like 2g lme for 500 ml.

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied]
    #29634857 -

1.2

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied]
    #29634864 -

Mugsey429 said:
Hey man i know this thread is old, I was just curious how much LME you'd add for the 300ml jars, i know you said you like 2g lme for 500 ml.


I like 0,2% DME(dry malt extract) for LC. So for 300ml of LC that would be 0,6g DME.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: The Biopsy Method: A different way of doing LC. [Re: Kloakk]
    #29634869 -

Gotcha thanks everyone, is dme different from lme?

Edited by Mortifiied (07/28/26 04:26 PM)

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied]
    #29634876 -

Mugsey429 said:
Gotcha thanks everyone, is dme different from lme?


Depends which community you ask :lol: Homebrewers will tell you that LME is liquid malt extract and DME is dry malt extract, here on the org LME is light malt extract and the dry part isn't mentioned because it's obvious I guess, I'm coming from homebrewing so I'm trying to convert everyone over to DME, mostly because I'm old and don't like change, but also because if anyone reading want to buy some light coloured dry malt extract they should search for DME and not LME.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: The Biopsy Method: A different way of doing LC. [Re: Kloakk]
    #29634880 -

Ok lol i gotcha, also what kinda of mushrooms are those bottom left in your sig? The tiny gray ones ttbvi I think it says, their beautiful.

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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #29634905 -

Again I know this thread is extremely old but it's all new to me, If I have a box of 150 brand new sterile 16g needles I dont need to bother heating the tip before doing the poke do I? Just connect it to my sterile syringe of water pull the cap off the new sterile needle and poke right?

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied] * 1
    #29634930 -

If it’s a brand name needle and labelled as sterile I wouldn’t heat it. If someone was going to draw a blood sample from me and heated the needle first I’d run.
If it makes you feel better to heat it that’s a different matter.

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Re: The Biopsy Method: A different way of doing LC. [Re: The Tao]
    #29634941 -

Yea I would not want to see that at the doctors office lol, I have no problem heating it with a syringe i haven't used all of the contents in but it def seems counter intuitive with a sealed sterile needle.

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied] * 3
    #29634947 -

this thread is shroomery legend.
:josex:


long live josex
:pope:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #29634964 -

Agreed I read 30 pages of the thread since last night and I am very excited for some future projects, also read a whole bunch on eat's slurry tek that i will also be implementing some time. Beefextreme Bod Pasty Kloak Eat Josex I'm sure i'm missing some but thank you everyone who gives their knowledge on this site, I stepped away for 10 years and hopefully in the next 7 days I'll have some beautiful fruits to show everyone.

Edited by Mortifiied (07/28/26 07:10 PM)

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied]
    #29634986 -

Think for josex poke u want a 21 to 23 gauge syringe tip not 16.

16 is very wide.more material transfered = higher contam chance

Id use 14 to 16 to suck up or dispense lc though I wouldn't do it for the initial josex poke to inoculate


--------------------





An enigma some caerulescens n a mono 😎

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied]
    #29635105 -

Mugsey429 said:
Ok lol i gotcha, also what kinda of mushrooms are those bottom left in your sig? The tiny gray ones ttbvi I think it says, their beautiful.


Thanks man, TTBVI is a Panaeolus.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: The Biopsy Method: A different way of doing LC. [Re: Kloakk]
    #29635130 -

Awesome man thanks, I ended up googling it like a should've done from the beginning but i appreciate it lol. Beautiful mushrooms, do you think their harder than b+ to grow? I know they like manure based substrates i think, and I have like 15lbs ready to grow that i bought to get back into the hobby.

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Re: The Biopsy Method: A different way of doing LC. [Re: Mortifiied]
    #29635154 -

Mugsey429 said:
Awesome man thanks, I ended up googling it like a should've done from the beginning but i appreciate it lol. Beautiful mushrooms, do you think their harder than b+ to grow? I know they like manure based substrates i think, and I have like 15lbs ready to grow that i bought to get back into the hobby.


Yes in some aspects pans a harder to grow then cubes, mostly because they need a nutritious substrate, a proper casing, higher temperatures and a good method for pasteurizing. For best results a automated fruiting setup is needed, but you can do pans in tubs and still get more then enough as they are very potent :awesomenod:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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