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Invisiblecpw1971
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need good LC tek pictorial
    #6358561 - 12/11/06 07:48 PM (14 years, 4 months ago)

hey guys
I was wondering if any of you can point me in the right direction for a good LC tek pictorial. I want to use mason jars and I have Karo light corn syrup or I can go get some Dextrose "corn sugar".
I have a pc "10psi" , coffee filters, Tyvek, antibacterial band aids, white medical tape, polyfill, aluminum foil, metal caps for jars, Ozium, a plastic handled scalpel, alcohol, hepa filter/neg ion gen, Industrial carbon filter with vortex fan, a clear bin to make a glovebox, extra long latex gloves, bleach, 10ml and 60 ml syringes "without needles yet but some are coming in mail" and silicone.
I basically need help setting up the mason jar "making the lid" and glovebox. And help using the Karo and storing the LC.
oh and I have 3 sizes of the jars.. 1/2 pint, 1 1/2 pint and quart.
thanks :-)


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Offlinechainsaw1298
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Re: need good LC tek pictorial [Re: cpw1971]
    #6358591 - 12/11/06 07:54 PM (14 years, 4 months ago)



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Invisiblecpw1971
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Re: need good LC tek pictorial [Re: chainsaw1298]
    #6358617 - 12/11/06 08:00 PM (14 years, 4 months ago)

oh sweet thanks a lot :-)
I tried searching but couldn't find one.


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Invisiblecpw1971
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Re: need good LC tek pictorial [Re: cpw1971]
    #6358624 - 12/11/06 08:01 PM (14 years, 4 months ago)

oh yea I forgot,
I am using a spore print


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Offlinexeallos
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Re: need good LC tek pictorial [Re: chainsaw1298]
    #6358625 - 12/11/06 08:01 PM (14 years, 4 months ago)

CreamCorn's LC Tek is very newb friendly, I have found great success with this method. However, I found my syringe biopsies were very poor (was using a very small gauge needle) and I had to resort to chopping off a small chunk of a fresh shroom stalk into my LC... what can I say... I was desperate. Worse comes to worse, do that in your glovebox and you'll be straight.


Edited by xeallos (12/11/06 08:02 PM)


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Invisiblecpw1971
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Re: need good LC tek pictorial [Re: xeallos]
    #6358689 - 12/11/06 08:15 PM (14 years, 4 months ago)

yeah I think I will try the first link chainsaw posted but instead of a spore syringe I will scrape a print in a glovebox.
I hope this works lol


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Re: need good LC tek pictorial [Re: cpw1971]
    #6358946 - 12/11/06 09:19 PM (14 years, 4 months ago)

I don't mean to hijack, but this may be relevant. I don't have any spores (used em' all up) but I do have two freshly fully colonized rye jars. Could I make a nutrient water jar like those described in the teks, and then just put some colonized rye in there instead of spores???


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Place the patty on a sesame seed bun, in accordance to the principle of balance as mentioned by the Buddha. This may be an ordinary bun, but a true ninja prefers to toast the bun lightly in order to better savor the flavor of his burger.


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OfflineQuake3
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Re: need good LC tek pictorial [Re: buddhasninja]
    #6359025 - 12/11/06 09:36 PM (14 years, 4 months ago)

Quote:

buddhasninja said:
I don't mean to hijack, but this may be relevant. I don't have any spores (used em' all up) but I do have two freshly fully colonized rye jars. Could I make a nutrient water jar like those described in the teks, and then just put some colonized rye in there instead of spores???




http://www.shroomery.org/forums/showflat.php/Number/6344136#6344136

RR posted this a few days ago:
Quote:


For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this.

Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.





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Invisibleodium33
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Re: need good LC tek pictorial [Re: buddhasninja]
    #6359027 - 12/11/06 09:37 PM (14 years, 4 months ago)

Quote:

buddhasninja said:
I don't mean to hijack, but this may be relevant. I don't have any spores (used em' all up) but I do have two freshly fully colonized rye jars. Could I make a nutrient water jar like those described in the teks, and then just put some colonized rye in there instead of spores???




do this in a glove box with relatively sterile conditions and yes it will indeed work


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Invisiblecpw1971
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Re: need good LC tek pictorial [Re: odium33]
    #6359094 - 12/11/06 09:53 PM (14 years, 4 months ago)

didn't someone do something like that in the hydroponic thread??
I forgot the name of the thread but basically a shroom started to grow in his solution.


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Invisibleodium33
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Re: need good LC tek pictorial [Re: cpw1971]
    #6359114 - 12/11/06 09:57 PM (14 years, 4 months ago)

that will happen if you leave it long enough. from what i understand, you just need to put the grain in the jar with sterile water, shake up to release mycelium from grain, and suck into syringe


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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon ½ Pint Jars, HEPA Filter, Hydroponics Supplies, Rye Grain, Wild Bird Seed

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