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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Edmunter]
    #27197251 - 02/10/21 01:55 PM (8 months, 5 days ago)

Be careful tho, some of those components may not be autoclavable, probably those little rings pictured aren't. I have one too, that's how I know. I have a friend that offered to tinker with the inoculation gun to make it autoclavable but I said fuggit, syringes are easy af too.

If you want a real autoclavable gun you probably need to shell out way more than that price tag.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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InvisibleWall.E
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27203108 - 02/13/21 08:35 AM (8 months, 2 days ago)

Quote:

Josex said:
Now I'm going to talk about your feelings.
After the poke, you may feel skeptical, as if you just did nothing, cuz no way in hell this is going to work...
You may feel an uncontrollable urge to stab the culture to death, cuz no way in hell this is going to work...
You may feel the impulse of scraping the culture after poking it, cuz no way in hell this is going to work...
This is all a crock of horseshit and the sooner you learn this the better.





Just realized I've never posted in this thread :rofl:

If you're reading this and debating whether or not this works. Or if Joe Sex is spewing bullshit about your feelings, let me show you what I - a total fuckin retard - was able to pull off following this tek.

Psilocybe natalensis



Psilocybe natalensis Tests (different lc that's already in jars)





Panaeleous Camb. (Jambos)



Psilocybe tampanesis



Pleurotus ostreatus






Ps. Cube. Amazon



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Life’s shit, but I’m loving it



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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: Wall.E]
    #27203178 - 02/13/21 09:53 AM (8 months, 2 days ago)

:neat:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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InvisibleWall.E
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27203183 - 02/13/21 09:55 AM (8 months, 2 days ago)

Thanks again ya brilliant bastard


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Invisibleshadyshroomie
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Re: Josex' Poke: A different way of doing LC. [Re: Wall.E]
    #27205627 - 02/14/21 04:22 PM (8 months, 1 day ago)

*Me thinking there is no way my poke got any mycelium in the needle*

*Me thinking the needle just stabbed a ring around a chunk of mycelium and came out clean with nothing in it*

*Me thinking no way this works I should stab again... then saying no fuck that and trusting it*

LC today 2/14:


Pics came out blurry but there is a decently large cluster of mycelium

Inoculated about 500ml LC on 2/7 with a PE6 culture from a swab with one transfer
Do not plan to use all 500ml, I really did this as a test run to see how it goes before I make several LCs of TAS and JMF clones

I might try to use this LC on a small amount of grain and hope it has at least one fruit I can clone. Any other suggestions?

Another option I am leaning towards more is to place a drop or two of LC on several agar dishes, let them grow out fully, see if any of them pin, clone a pin from a dish, grow a clean culture of that clone, then use that culture to make an LC of PE6. I assume this would allow me to know the genetics have fruiting potential and thus potentially be a better initial PE grow rather than starting from the current LC I have going now.

Regardless... it was cool to have my doubts the first time trying it and instead of giving in to my urges to fuck with it, I just trusted it, and it worked like a charm


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Offlinemcnugget
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Re: Josex' Poke: A different way of doing LC. [Re: shadyshroomie]
    #27205722 - 02/14/21 05:10 PM (8 months, 1 day ago)

Going to give this technique a shot tonight and make my first lc.  Smallest needle I have is 16g so hoping all the water doesn't boil out. 

Wish me luck.


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InvisiblefahtsterM
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Re: Josex' Poke: A different way of doing LC. [Re: mcnugget]
    #27206338 - 02/14/21 11:07 PM (8 months, 18 hours ago)

Having good luck doing a poke on inner stem tissue through a SHIP!  First one colonized great.. currently drying it out for later use (I dry them broken up so they dry faster and aren’t a bitch to break apart later when I use them for glc)



I am finding that it works with grain much better if you can see some clone material lodged in the needle barrel.. super easy to do if you poke the needle with the grain of the inner tissue (long ways). Usually get some in the first poke.  Here’s a pea+ F2 clone and I have another PE clone that’s just starting to show myc growth.



Have you tried doing a poke through a SHIP on a LC jar, josex?

Faht


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EVERYTHING FAHT, CLICK ME!




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OfflinetiptrippyS
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Re: Josex' Poke: A different way of doing LC. [Re: fahtster]
    #27206362 - 02/14/21 11:29 PM (8 months, 17 hours ago)

Any recommendations on stirring if I don't have a stir plate yet? I'm using plastic lids with small SFD holes so I can't really shake them either. How often should I stir?


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Re: Josex' Poke: A different way of doing LC. [Re: tiptrippy]
    #27206613 - 02/15/21 03:11 AM (8 months, 14 hours ago)

In jars I shook it took 14 days to colonise but in the ones I could only swirl it took 2 weeks..........


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InvisibleWall.E
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Re: Josex' Poke: A different way of doing LC. [Re: Edmunter]
    #27206765 - 02/15/21 06:45 AM (8 months, 10 hours ago)

Quote:

Edmunter said:
In jars I shook it took 14 days to colonise but in the ones I could only swirl it took 2 weeks..........




:joneswtf:

Anyways, i swirl them and it seems to work out fine


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: Wall.E]
    #27206793 - 02/15/21 07:19 AM (8 months, 10 hours ago)

Noice Faht! That's an interesting application, thanks for posting.

Yea I knew you can squirt the tissue through a SHIP, some people in this thread tried it with success and I noc my LC's through a thick poly filter routinely.


I don't think LC needs to be shaken, there's no need for that. Good ol swirling does fantastic if you're not using magnetic stirrers. My jars have a silicone tube for aspirating that also happens to help to break up the myc even with gentle swirling, but you can introduce things in the jar like a blender ball (that's what I usee to do) and many people use marbles or pieces of broken glass (never tried that).


Nothing in the jar to break the myc ain't the end of the world either and swirling would also be plenty good in this case, especially if you're planning to pour the LC.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: Josex' Poke: A different way of doing LC. [Re: Wall.E]
    #27207016 - 02/15/21 10:44 AM (8 months, 6 hours ago)

Quote:

Myc_Hunt said:
Quote:

Edmunter said:
In jars I shook it took 14 days to colonise but in the ones I could only swirl it took 2 weeks..........




:joneswtf:

Anyways, i swirl them and it seems to work out fine




And if you do nothing Myc it takes half a month bruv


Edited by Edmunter (02/15/21 10:44 AM)


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Re: Josex' Poke: A different way of doing LC. [Re: Edmunter]
    #27207040 - 02/15/21 11:03 AM (8 months, 6 hours ago)

I heard if you do nothing it’s a fortnight


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OfflineEdmunter
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Re: Josex' Poke: A different way of doing LC. [Re: A.k.a]
    #27207097 - 02/15/21 11:40 AM (8 months, 5 hours ago)

Quote:

A.k.a said:
I heard if you do nothing it’s a fortnight




Rumours......I heard it was 336 hours....

Hows it going AKA, you are tagged up...nice.

Im loving this mix with Agar in it.  It just suspends the culture wherever it the swirl stops.

Also test plates are a great way of getting a new plate ready for a new poke.....

Also Josex whats the quickest youve had one colonise.  I have a clone going mental, 6 days old.  I reccon it will be ready in 10


Edited by Edmunter (02/15/21 11:42 AM)


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: Edmunter]
    #27208239 - 02/15/21 10:17 PM (7 months, 30 days ago)

Yeah my personal record has been 6 days too for quarts, from LC in a hot summer. I only had it faster than that (4-5 days) from PF-tek slurries back when I used to do them.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27208258 - 02/15/21 10:30 PM (7 months, 30 days ago)

What’s up man, you took a long break!


My poke lcs took a while to colonize, but lc to grain I must’ve hit everything just right cuz I knocked up five no prep jars with whyte gold and one finished in five days. It would be so nice to get that consistently.


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OfflineDontMatter
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Re: Josex' Poke: A different way of doing LC. [Re: A.k.a]
    #27214907 - 02/19/21 03:40 PM (7 months, 27 days ago)

Hey guys,

anyone already tried to just suck the poke right into a previously sterilized grain-water-filled syringe?

i'm about to try that and wondering if theres already experience to it...

my thinking is that doing it this way one would skip time and the risk of contam by colonizing directly inside the syringe you are going to inoc with


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: DontMatter]
    #27214923 - 02/19/21 03:50 PM (7 months, 27 days ago)

Yes it works, but it only takes doing it once to know not to do it again.
I did some tests not long ago with 10ml syringes where I aspirated the biopsy using sterile water. Some never took inside the syringes and the ones who did took way too long to colonize (which was counterintuitive considering they were 10ml syringes).
Also, growth looked very wispy and weak, nothing to do with a standard LC. On top of that it's a very ghetto way of doing things, LC requires higher standards.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineDontMatter
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27214946 - 02/19/21 04:03 PM (7 months, 27 days ago)

maybe due to lack of GE inside the syringe?

considering your answer i'll do a normal LC and try the LC-syringe just to get that same experience lol

thanks for your fast reply:grin:


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Invisiblemicelio
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Re: Josex' Poke: A different way of doing LC. [Re: DontMatter]
    #27215059 - 02/19/21 05:07 PM (7 months, 27 days ago)

Maybe the lack of nutrients..


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