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Offlinerockyfungus
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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27448967 - 08/30/21 06:54 AM (3 months, 3 days ago)

That's because if we want to get pedantic it would be leucesitic golden teachers.


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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: rockyfungus]
    #27449000 - 08/30/21 07:52 AM (3 months, 3 days ago)

Quote:

rockyfungus said:
That's because if we want to get pedantic it would be leucesitic golden teachers.




no results from TFSE


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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby] * 1
    #27449228 - 08/30/21 11:35 AM (3 months, 3 days ago)



PC'ing a bunch of syringes with water.

Pokes for months! :dancer:


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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27449291 - 08/30/21 12:29 PM (3 months, 3 days ago)

Lol I was wondering how to fit a syringe into my pc vertically, but it was too tall so I went horizontal. In the end should've just used half the water :facepalm3:

Can you keep reusing a syringe after you used once and capped it, or should you resteralize?


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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27449406 - 08/30/21 02:06 PM (3 months, 3 days ago)

Quote:

Ora said:
Can you keep reusing a syringe after you used once and capped it, or should you resteralize?




Theoretically you could do that and be fine, but I never liked it. Before having a hood my policy was one syringe, one use.

I sterilize and keep the syringes in the jar pictured, and now that I work in the Jo-flow I don't mind putting used syringes back in the jar for a second use later on.


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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27449430 - 08/30/21 02:19 PM (3 months, 3 days ago)

I flame my needle and wait for it to be red hot and suck some air in. Is that technically staying sterile? Then push a little water out in SAB and poke again. Not ideal but has worked.

I now use 1 syringe per poke/LC/GLC


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OfflineOra
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Re: Josex' Poke: A different way of doing LC. [Re: rockyfungus]
    #27449440 - 08/30/21 02:27 PM (3 months, 3 days ago)

ill probably just make a new one then.

why would you want to suck in any air? doesnt sound sterile since your getting stuff into the actual syringe.

i used 1 syringe for multiple pokes on diff plates with flaming in between. dont see why you would need 1 syringe for 1 poke unless your being super careful.


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Offlinerockyfungus
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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27449447 - 08/30/21 02:33 PM (3 months, 3 days ago)

Quote:

Ora said:
ill probably just make a new one then.

why would you want to suck in any air? doesnt sound sterile since your getting stuff into the actual syringe.

i used 1 syringe for multiple pokes on diff plates with flaming in between. dont see why you would need 1 syringe for 1 poke unless your being super careful.




Sorry sucking in red hot air. Most of my plates are 1 little piece of white and green everywhere else. So super cautious and rather just use a ton of needles to limit odds of cross contam.


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Re: Josex' Poke: A different way of doing LC. [Re: rockyfungus]
    #27449458 - 08/30/21 02:37 PM (3 months, 3 days ago)

I looked up some stats about how much microbial crap is floating in the air, and found a study that was conducted at a daycare. They found about one CFU per 50 mL of air, if I'm remembering right. So air isn't as thick with contams as I'd thought. Of course, it's probably much better if you run a good air purifier like a flow hood, and it's probably much much worse if mold big enough to see is sporulating.

So drawing some air into your syringe won't be an issue, most of the time. But you can draw air from a flame if you really want to draw sterile air. If that's what you're doing, it's pretty good. Though as the air in the tube cools, it will suck in more air.


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Re: Josex' Poke: A different way of doing LC. [Re: rockyfungus]
    #27449460 - 08/30/21 02:37 PM (3 months, 3 days ago)

still not sure why you want to suck in air though, doesnt seem safe and i dont know why you do it.


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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27449463 - 08/30/21 02:38 PM (3 months, 3 days ago)

Quote:

Ora said:
ill probably just make a new one then.

why would you want to suck in any air? doesnt sound sterile since your getting stuff into the actual syringe.

i used 1 syringe for multiple pokes on diff plates with flaming in between. dont see why you would need 1 syringe for 1 poke unless your being super careful.




One syringe for one sesh/multiple pokes, that's what I used to do.

Again, even if you work in a SAB, you could cap it and put it back in the jar for later use and be fine, it's just that I personally don't like to do it. OCD is strong in this one.


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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27449466 - 08/30/21 02:39 PM (3 months, 3 days ago)

Quote:

Ora said:
still not sure why you want to suck in air though, doesnt seem safe and i dont know why you do it.




Yeah I didn't understand that either.


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Offlinerockyfungus
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27449535 - 08/30/21 03:21 PM (3 months, 3 days ago)

I guess it's an OCD thing from hearing you could do it with spore syringes to save them. It's not something I do regularly. But feels safer then just capping if I'm reusing a syringe.


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Re: Josex' Poke: A different way of doing LC. [Re: rockyfungus]
    #27449539 - 08/30/21 03:25 PM (3 months, 3 days ago)

oh like to get rid of the water in the needle or something? usually when i flame the needle the water spurts out, so maybe thats enough to dry the needle


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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27463591 - 09/10/21 11:41 PM (2 months, 22 days ago)

So I did a poke LC, let it go for 9 days, has a whole bunch of mycelium in it. Then I did a pour of about 10cc each to 5 jars. 2 days later and I'm seeing nothing at all. Does that make any sense? I've never done a LC noc before, I thought it would be similar to g2g where i see A LOT in a day.


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Re: Josex' Poke: A different way of doing LC. *DELETED* [Re: Josex]
    #27480837 - 09/24/21 03:39 PM (2 months, 9 days ago)

Post deleted by savan73

Reason for deletion: .


Edited by savan73 (09/24/21 03:39 PM)


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Offlinewahhwahh
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Re: Josex' Poke: A different way of doing LC. [Re: wahhwahh]
    #27480954 - 09/24/21 05:53 PM (2 months, 9 days ago)

Quote:

wahhwahh said:
So I did a poke LC, let it go for 9 days, has a whole bunch of mycelium in it. Then I did a pour of about 10cc each to 5 jars. 2 days later and I'm seeing nothing at all. Does that make any sense? I've never done a LC noc before, I thought it would be similar to g2g where i see A LOT in a day.




Update to this, 3 pint jars fully colonized, threw those to sub. The two quart jars still showed literally nothing, so I threw in 25cc(!!) more in each 3 days ago and still nothing. No contam. No pooling. Nothing. Wtf haha


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Re: Josex' Poke: A different way of doing LC. [Re: savan73]
    #27480957 - 09/24/21 05:55 PM (2 months, 9 days ago)

Last link in my sig is a multi-growlog where all I did was poke germination plates directly to LC, no transfers, so yeah you def can. In the growlog, pf-tek pucks were used instead of agar though.

Good thing about LC is that if contamination gets introduced at inoculation you're going to end up with an unusable LC which you're very likely to spot just by visual inspection even before testing it, LC doesn't forgive anything at all.

So yeah, I say poke germ plates to have something going on to keep you busy while you keep transferring the prettiest/fastest sectors to try and "improve" the culture.

More likely than not tho, you'll end up cloning something worthwhile from a successful grow done from a germ plate.


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Re: Josex' Poke: A different way of doing LC. [Re: wahhwahh]
    #27480961 - 09/24/21 05:58 PM (2 months, 9 days ago)

Quote:

wahhwahh said:
Quote:

wahhwahh said:
So I did a poke LC, let it go for 9 days, has a whole bunch of mycelium in it. Then I did a pour of about 10cc each to 5 jars. 2 days later and I'm seeing nothing at all. Does that make any sense? I've never done a LC noc before, I thought it would be similar to g2g where i see A LOT in a day.




Update to this, 3 pint jars fully colonized, threw those to sub. The two quart jars still showed literally nothing, so I threw in 25cc(!!) more in each 3 days ago and still nothing. No contam. No pooling. Nothing. Wtf haha




Those will grow too I think, just give it another day or two.

Sometimes if you prep the grain overly dry, the culture might have a hard time to recover and leap off. The same thing may happen if you overcook the grain by doing too long a cycle or at very high pressures.


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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27481067 - 09/24/21 08:04 PM (2 months, 8 days ago)

i had lots of dry millet jars stalling out at the 3 week mark, so i dumped like 30cc or more of LC with poke method and now its like day 3 and lots of mycellium are popping up. probably gonna explode in the next day or 2 :thumbup:


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