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Invisiblep9hu7
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Re: The Biopsy Method: A different way of doing LC. [Re: SpaceBaby]
    #27435268 - 08/19/21 11:44 PM (1 month, 25 days ago)

No need to toss it, It just seemed like a lot.

I use .5gr LME/500ml distilled water. It makes crystal clear broth that makes for spotting contamination very easy.



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OfflineSpaceBabyS
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Re: The Biopsy Method: A different way of doing LC. [Re: p9hu7] * 1
    #27435269 - 08/19/21 11:45 PM (1 month, 25 days ago)

Quote:

p9hu7 said:
No need to toss it, It just seemed like a lot.

I use .5gr LME/500ml distilled water.




Alright, I'll let it run, for Alchemy. I mean Science. :bow2::rofl:


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


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Invisiblep9hu7
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Re: The Biopsy Method: A different way of doing LC. [Re: SpaceBaby]
    #27435274 - 08/19/21 11:50 PM (1 month, 25 days ago)

I think that once you see how clear your broth is you'll never go back to amber broth again.


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OfflineSpaceBabyS
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Re: The Biopsy Method: A different way of doing LC. [Re: p9hu7]
    #27435383 - 08/20/21 01:32 AM (1 month, 25 days ago)

Quote:

p9hu7 said:
I think that once you see how clear your broth is you'll never go back to amber broth again.




you're usually right, p9, so...prolly :hatsoff:

I quite liked the growth off the LI. I threw a pretty T1 into the 100ml distillee H20 and noccc'd 7 jars of millet tonight. It's a little drier than last time, but I have a feeling that is a good thing.


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


Edited by SpaceBaby (08/20/21 01:34 AM)


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: bboydamn]
    #27436091 - 08/20/21 04:49 PM (1 month, 25 days ago)

Quote:

bboydamn said:
first thank you, now i understand the faith issue 😅 of 3 bottles 2 worked.  Another thing: you can indicate a link to make a cover like yours.  I didn't understand how to do it




You can poke more than once, there's no issue with that. This TEK was made years ago and for whatever stupid reason I found it important to capitalize on the phrase "poke once". Pay no attention to n00b me from time ago and poke 1, 2 or 3 times. If you only poke once you'll get no growth at all sometimes.

There's no link to that lid cover because I never made a TEK, sorry.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27436398 - 08/20/21 09:02 PM (1 month, 25 days ago)

Quote:

Josex said:
Quote:

bboydamn said:
first thank you, now i understand the faith issue 😅 of 3 bottles 2 worked.  Another thing: you can indicate a link to make a cover like yours.  I didn't understand how to do it




You can poke more than once, there's no issue with that. This TEK was made years ago and for whatever stupid reason I found it important to capitalize on the phrase "poke once". Pay no attention to n00b me from time ago and poke 1, 2 or 3 times. If you only poke once you'll get no growth at all sometimes.

There's no link to that lid cover because I never made a TEK, sorry.



hmm, i noccd 2 broth . crazy heavy nutes cause :shrug: i poked once. one i even remembered to put a stir bar in. :rolleyes:

here they are 18 hours after nocc



l8r


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SpaceBaby
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Cult Queen:

Another Day's Work:


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Offlinekarri0n
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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27436437 - 08/20/21 09:39 PM (1 month, 25 days ago)

Rockin the labelmaker, good shit.


--------------------

Anything written above is just as likely to be accurate as it is to be corrected by someone smarter than me two posts from now.


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: karri0n]
    #27436876 - 08/21/21 08:30 AM (1 month, 24 days ago)

:whathesaid:

What an ego trip to see "JosexPoke" in a label somewhere else in the world, tyvm.
:etjesus:

Poking only once works most times, but sometimes people poke the edges of the culture where the colony isn't yet well established in the agar puck and that can fail.

I've also noticed that when growth is wispy and/or weak, poking only once may fail too.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: Josex] * 1
    #27437094 - 08/21/21 11:51 AM (1 month, 24 days ago)

Quote:

Josex said:
:whathesaid:

What an ego trip to see "JosexPoke" in a label somewhere else in the world, tyvm.
:etjesus:

Poking only once works most times, but sometimes people poke the edges of the culture where the colony isn't yet well established in the agar puck and that can fail.

I've also noticed that when growth is wispy and/or weak, poking only once may fail too.




pride of appreciation is good.
i appreciate you.


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27437295 - 08/21/21 02:45 PM (1 month, 24 days ago)

You're too good man! :hug:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27437713 - 08/21/21 09:36 PM (1 month, 24 days ago)

Quote:

Josex said:
You're too good man! :hug:




awww

dood, if you knew my history…

but thanks.

:kittylaugh:


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


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OfflineGuerrilla
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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27438721 - 08/22/21 06:12 PM (1 month, 23 days ago)

Could this work for new growth as young as this? I had a clone plates parafilm rip, this is the transfer from that plate. I was hoping I could do what mushboy did and shoot onto an agar plate and also an LC before any potential contamination appears.. I understand transferring a clone isn’t the best thing to do but I was hoping to save the genetics.



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Yup it’s me again. Shit, I post too much..

fahtster said:
The only bad experience is the one not had.


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Offlinegone-pear-shaped
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Re: Josex' Poke: A different way of doing LC. [Re: Guerrilla]
    #27438993 - 08/23/21 12:13 AM (1 month, 22 days ago)

If you want to keep the culture, you have to transfer it, at least to a slant, then again from the slant to a master plate and child plate... and I don't see a problem with using the plate to make an LC after the transfer. Do the transfer first if that's your higher importance for cleanliness.


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OfflineBrownBear
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Re: Josex' Poke: A different way of doing LC. [Re: gone-pear-shaped]
    #27439017 - 08/23/21 01:03 AM (1 month, 22 days ago)

Imo, the lc should be the first transfer. A transfer to another plate can be cleaned up but an lc cannot. Just my :2cents:


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Re: Josex' Poke: A different way of doing LC. [Re: BrownBear]
    #27445792 - 08/28/21 01:19 AM (1 month, 17 days ago)

i made 4 bottles of lc using brf today using 1000g water and 4g brf simmered for 10 min then only took the liquid after the particles settled on the bottom. im wondering if i should redo it because i didnt add agar to it. or do you think the starch from the brf will do the same thing as the agar?


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Offlinegone-pear-shaped
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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27445795 - 08/28/21 01:23 AM (1 month, 17 days ago)

It's not the way of this tek, but filtered grain water is fine in general. Letting it settle should be the same.


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OfflineOra
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Re: Josex' Poke: A different way of doing LC. [Re: gone-pear-shaped]
    #27445804 - 08/28/21 01:40 AM (1 month, 17 days ago)

yeah i just read through the tek and agar is not mentioned. i had read a post by josex about adding agar and it sped colonization to like 5 days for lc, but not related to the poke i guess.


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Offlinegone-pear-shaped
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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27445822 - 08/28/21 02:13 AM (1 month, 17 days ago)

Oh, sorry I misread. Even Josex himself uses agar now. I think he uses 0.125%. Some use 0.2%. It really depends on your agar, since it can have wildly different characteristics depending on how it was processed. I also want to try gelatin or carbomer so I can be sure the gel doesn't get damaged from being on a stir plate. Since as we know, agar gel breaks in some circumstances.

Yes, I'd add the thickening agent and re-sterilize. It seems to colonize faster.


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OfflineOra
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Re: Josex' Poke: A different way of doing LC. [Re: gone-pear-shaped]
    #27445832 - 08/28/21 02:42 AM (1 month, 17 days ago)

cool gotcha


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OfflineOra
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Re: Josex' Poke: A different way of doing LC. [Re: Ora]
    #27446559 - 08/28/21 06:04 PM (1 month, 17 days ago)

i did some poking into lc today. i may have messed 3/4 though. after the poke i only dropped like 2 drops of liquid into the lc, then finally on the last one i was like OH WAIT im supposed to squirt it.

so how reliable is this "microscopic amount of mycellium" needed to grow? cause i definetly got microscopic levels by dropping 2 drops, but idk. the last one i stabbed a super rhizo single strand and it stuck to the end of the needle, that was when i tried dropping 2 drops again but i saw the mycellium was still stuck to the needle, so i squirted and it came off.

i also squirted on the glass. but i shook it after, should be good right?


Edited by Ora (08/28/21 06:06 PM)


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