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Offlinewahhwahh
stupid idiot


Registered: 07/19/21
Posts: 296
Last seen: 2 months, 1 day
Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27481204 - 09/24/21 10:58 PM (2 months, 12 days ago)

Quote:

Josex said:
Quote:

wahhwahh said:
Quote:

wahhwahh said:
So I did a poke LC, let it go for 9 days, has a whole bunch of mycelium in it. Then I did a pour of about 10cc each to 5 jars. 2 days later and I'm seeing nothing at all. Does that make any sense? I've never done a LC noc before, I thought it would be similar to g2g where i see A LOT in a day.




Update to this, 3 pint jars fully colonized, threw those to sub. The two quart jars still showed literally nothing, so I threw in 25cc(!!) more in each 3 days ago and still nothing. No contam. No pooling. Nothing. Wtf haha




Those will grow too I think, just give it another day or two.

Sometimes if you prep the grain overly dry, the culture might have a hard time to recover and leap off. The same thing may happen if you overcook the grain by doing too long a cycle or at very high pressures.





Yeah, they are def a bit dry. I only used other ones from the quart run as this as g2g so far. The fact adding so much more LC cause no pooling... lol


Edited by wahhwahh (09/24/21 11:00 PM)


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Offlinekarri0n
No Title
Male User Gallery


Registered: 08/29/20
Posts: 578
Last seen: 1 day, 22 hours
Re: Josex' Poke: A different way of doing LC. [Re: wahhwahh]
    #27481726 - 09/25/21 01:48 PM (2 months, 12 days ago)

I poked an APE fruit then poked a clean plate to attempt a clone.

I then poked the same fruit then squirted about 1cc sterile water directly to a wbs jar.

I then poked clean growth on a PF redspore plate and injected sterile water to a wbs jar.

Will update with results.


--------------------
Micropore tape? More like myco-poor tape, right? HAR HAR


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InvisibleaNewBeginning

Registered: 06/26/21
Posts: 692
Re: Josex' Poke: A different way of doing LC. [Re: karri0n]
    #27542241 - 11/13/21 09:58 PM (24 days, 22 hours ago)

I'm trying this today.

How much should I let the mycelium grow before using it as an inoculant?


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Ask me about mind goblins


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OfflineSpaceBabyS
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 1,256
Loc: My Own Private Idaho
Last seen: 11 hours, 6 minutes
Re: Josex' Poke: A different way of doing LC. [Re: aNewBeginning]
    #27542279 - 11/13/21 10:53 PM (24 days, 21 hours ago)

Quote:

karri0n said:
I poked an APE fruit then poked a clean plate to attempt a clone.

I then poked the same fruit then squirted about 1cc sterile water directly to a wbs jar.

I then poked clean growth on a PF redspore plate and injected sterile water to a wbs jar.

Will update with results.





:takingnotes:


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


MUSHBOY'S SHROOM TEA TEK


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InvisibleaNewBeginning

Registered: 06/26/21
Posts: 692
Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27542425 - 11/14/21 01:04 AM (24 days, 19 hours ago)

I inoculated 3 jars.

2 jars from an APE plate and 1 jar from a tampanensis plate.

I have confidence in the APE jars.

When the tampanensis plate was poked, the plate slid a little bit. I didn't double check that it was on a completely flat surface (stoopid cookie rack).

Regardless, this TEK was even easier than I thought.

I don't want to get excited prematurely but if this works, this is a huge game changer.

Will post updates soon :laugh:


--------------------
Ask me about mind goblins


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InvisibleMtbromo
The Bro


Registered: 08/27/20
Posts: 186
Re: The Biopsy Method: A different way of doing LC. [Re: aNewBeginning]
    #27563925 - 12/01/21 12:02 AM (7 days, 20 hours ago)

Brroo you a mofucking genius!!! Wwhhaattt! #mindblown


--------------------
Knowledge and Healing in a Handful.


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Invisiblepablokabute
Hari ng Amag
 User Gallery


Registered: 11/22/11
Posts: 4,765
Loc: rural ghetto
Re: Josex' Poke: A different way of doing LC. [Re: aNewBeginning]
    #27563970 - 12/01/21 12:48 AM (7 days, 19 hours ago)

Quote:

aNewBeginning said:
I inoculated 3 jars.

2 jars from an APE plate and 1 jar from a tampanensis plate.

I have confidence in the APE jars.

When the tampanensis plate was poked, the plate slid a little bit. I didn't double check that it was on a completely flat surface (stoopid cookie rack).

Regardless, this TEK was even easier than I thought.

I don't want to get excited prematurely but if this works, this is a huge game changer.

Will post updates soon :laugh:





I know i read it somewhere and totally forgot, but, whats the significance of using cookie rack again? Thanks if you can respond.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



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InvisibleDrboomer
The lord magnificent
I'm a teapot

Registered: 09/22/19
Posts: 720
Re: Josex' Poke: A different way of doing LC. [Re: pablokabute]
    #27563976 - 12/01/21 12:58 AM (7 days, 19 hours ago)

Raising work off the bottom of the sab. Which is where by virtue of it's design contamination settles


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OfflineEdmunter
Mr
Male User Gallery


Registered: 05/01/13
Posts: 5,684
Last seen: 7 days, 4 hours
Re: Josex' Poke: A different way of doing LC. [Re: Drboomer]
    #27564717 - 12/01/21 02:58 PM (7 days, 5 hours ago)

This tek is banging if you use 1500g of millet in bags.  Stab, inoc lc, inoc bag, tape over....repeat.....  I have 12-20 lcs going all the time

To add, when I make the syringe to inoc grains I also test a plate,  This the continues on the culture.  You can also retest and use the same syringe


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Offlineseldom seenS
 User Gallery


Registered: 11/03/07
Posts: 700
Last seen: 4 hours, 19 minutes
Re: Josex' Poke: A different way of doing LC. [Re: Edmunter]
    #27564730 - 12/01/21 03:10 PM (7 days, 5 hours ago)

This is what got me into LC, it's a thing of beauty.


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