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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: nix21]
    #27375990 - 07/06/21 10:20 AM (2 months, 11 days ago)

Hey guys of all genders,

So this is my Josex' Poke LC from way back. Been colonized and been swirling. Wanted to nocc millet with it later today.

Is it looking funky? What's going on in there. I don't know if I can test it on agar cause once it's open I figure byebye, right? It's okay if oats fuck up but FFS millet is $2/lb!

20210530:




Is that just pinning in there?!? Or is it trash? I also have a wedge drop LC in a media bottle. Can I aspirate such to test on agar and be able to use it again?

20210530:


This is a wedge drop from 20210604:




Diagnoses, please? Yah, I know we can't tell from looking. I did get a couple proper LC lids from shroomsupply. I know we mostly make all our shit, but I got an art business to run, too.

tyia

Space


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: nix21]
    #27375993 - 07/06/21 10:24 AM (2 months, 11 days ago)

Quote:

nix21 said:
If i wanna do more then one LC with same needle and syringe should i sanitize syringe with alcohol between every needle flaming and poking?




I never do that but if doesn't hurt if you do.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27375998 - 07/06/21 10:29 AM (2 months, 11 days ago)

Space, to my eye nothing indicates that the first LC is bad, but we can't be sure from a pic. Looks like a healthy, thick LC that's a bit grown out.

The others are just way too dark to make out anything, pics are usually terrible to judge cleanliness of an LC.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27376006 - 07/06/21 10:34 AM (2 months, 11 days ago)

Quote:

Josex said:
Space, to my eye nothing indicates that the first LC is bad, but we can't be sure from a pic. Looks like a healthy, thick LC that's a bit grown out.

The others are just way too dark to make out anything, pics are usually terrible to judge cleanliness of an LC.



Yeah, I know...the first is your poke into specifically prepped broth.

The other two...:facepalm3: :facepalm3:...agar prep that I forgot the agar for. :rofl:

I'm just goona throw caution, and maybe $$$, to the winds and nocc 10 millet with the 150 ml of thick JoPo LC.

Wim Hof breath hold begins.


--------------------
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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27376010 - 07/06/21 10:36 AM (2 months, 11 days ago)

Just don't wim Hof hold for too long.

The darker LC's do look suspect though, but it could be you just used way too much nutes to make the broth so it's very dark and cloudy.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OfflineSpaceBabyS
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Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #27376014 - 07/06/21 10:38 AM (2 months, 11 days ago)

Quote:

Josex said:
Just don't wim Hof hold for too long.




Haven't been at it for a while.
Have medical O2 which I used for WH breathing once and got 2 SIX MINUTE breath holds. Cool AF. Felt like a mutant. lol


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

tla Reine de la Maison:

porn:


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Invisiblenix21
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Re: Josex' Poke: A different way of doing LC. [Re: SpaceBaby]
    #27378839 - 07/08/21 11:05 AM (2 months, 9 days ago)

I think i will poke this T2 in 2-3 days for my first try doing this method, that satellite you see is just agar peace from transfer, wish me luck :wink: i was waiting to long to try this


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OfflineFridgedoor
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Re: Josex' Poke: A different way of doing LC. [Re: nix21]
    #27388162 - 07/15/21 03:11 AM (2 months, 2 days ago)

Thank you Josex for this write up,this is pure gold!

I never looked up on how to make LC and the very first thing I found, when I finally did, was your tek. I got everything I need and will give it a try!

Edit: I decided to do the grain broth you like so much. I just got a question regarding the LC jar. I got these wide mouth mason jars with a metal lid a a ring to screw it on tightly. Now when I want to sterilize the jar with the broth, will I have to flip the lid and screw on the ring tightly or loose, in order to avoid pressure build up? Or can I just use one of my modified jars I use for my grain spawn (metal lid with hole covered with micropore tape for GE)?


--------------------
Trip. Here. Now.

You are an aperture through which the universe is looking at and exploring itself. - Alan Watts

IDDQD


Edited by Fridgedoor (07/15/21 05:56 AM)


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Offlinebboydamn
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #27413667 - 08/04/21 01:27 AM (1 month, 13 days ago)

how do you test LC on agar?  how to remove LC from jar to test on agar plates?


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: bboydamn] * 1
    #27413791 - 08/04/21 08:04 AM (1 month, 13 days ago)

Quote:

Fridgedoor said:
Thank you Josex for this write up,this is pure gold!

I never looked up on how to make LC and the very first thing I found, when I finally did, was your tek. I got everything I need and will give it a try!

Edit: I decided to do the grain broth you like so much. I just got a question regarding the LC jar. I got these wide mouth mason jars with a metal lid a a ring to screw it on tightly. Now when I want to sterilize the jar with the broth, will I have to flip the lid and screw on the ring tightly or loose, in order to avoid pressure build up? Or can I just use one of my modified jars I use for my grain spawn (metal lid with hole covered with micropore tape for GE)?




Some people use unmodified lids for LC with success but I don't recommend it because you have to take certain precautions which are a bit of a nuisance that could be entirely avoided with the use of a filter.

I barely trust mp tape for anything and even less for LC. It's gonna get pretty wet and it's not a good filter to begin with. SDF's (whole or cut out) will work, as well as properly applied/stuffed polyfil and of course, a watman filter that can be purchased cheaply on the internet.



Quote:

bboydamn said:
how do you test LC on agar?  how to remove LC from jar to test on agar plates?




I take it your lids are not designed for aspirating into syringes? In that case you can use an inoculation loop to do the job.

Flame the inoc loop, crack the LC lid and quickly reach with the inoc loop into the LC, then streak on agar.

Sometimes with this method you won't see any growth on agar at all because it's possible you didn't catch any mycelium with the inoc loop. But even when no myc takes on the agar, if no bacteria grew either you can confidently say the LC is clean, because if there was bacteria it would show quickly regardless if there was myc growth or not.

Eventually, if you plan on sticking with heavy LC use, the goal is become good enough at it that you trust yourself and  don't even feel the need to test them.

I never test them myself, the few times I did get a bad LC I could tell with ease just looking at it and I'm willing to take the chances (directly going to grain) if for whatever reason that one-off bad LC escapes my visual inspection, but I can't remember that happening besides that time that I got a moldy LC that got the OK from me.
Bacteria in LC is usually much easier to spot than mold, though.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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OnlineCamera93S
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #27420358 - 08/08/21 11:11 PM (1 month, 8 days ago)

Praise to Jo! Me of little faith

The turbid looking ones are w/ agar.
JoPoked nocced the broths on 8/2. I expect them to really explode in this coming week.

Great stuff Josex!  :pope:

[url=https://files.shroomery.org/files/21-31/847838393-20210808_230127.jpg]


--------------------
It was realer then I imagined

All I need are some tasty waves, a cool buzz, and I’m fine.


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Offlinebboydamn
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Re: Josex' Poke: A different way of doing LC. [Re: Camera93]
    #27420495 - 08/09/21 03:10 AM (1 month, 8 days ago)

thank you very much for your attention.  this topic is sensational.  I want to make changes like you.  do you have a tutorial that can show you how to do it?


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InvisibleJosex
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Re: Josex' Poke: A different way of doing LC. [Re: bboydamn]
    #27420705 - 08/09/21 10:33 AM (1 month, 8 days ago)

:rockon:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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InvisibleMateja
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Re: The Biopsy Method: A different way of doing LC. [Re: Camera93]
    #27421470 - 08/09/21 07:26 PM (1 month, 7 days ago)

Quote:

Camera93 said:
Praise to Jo! Me of little faith

The turbid looking ones are w/ agar.
JoPoked nocced the broths on 8/2. I expect them to really explode in this coming week.

Great stuff Josex!  :pope:

[url=https://files.shroomery.org/files/21-31/847838393-20210808_230127.jpg]



Is this like a side by side test with regular LC broth vs Agar LC? :popcorn:


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Water Tub


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OnlineCamera93S
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Re: The Biopsy Method: A different way of doing LC. [Re: Mateja]
    #27421664 - 08/09/21 09:40 PM (1 month, 7 days ago)

It could have been if I had really measured or documented lol
I just wanted to try it with and without


--------------------
It was realer then I imagined

All I need are some tasty waves, a cool buzz, and I’m fine.


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Offlinerockyfungus
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Re: The Biopsy Method: A different way of doing LC. [Re: Camera93]
    #27422134 - 08/10/21 08:23 AM (1 month, 7 days ago)

Anyone else loving the poke for transfers even?

From:

To:

So, yeah, been fighting green all summer, and without the Poke I would of burned the whole house down.


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Offlinekarri0n
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Re: The Biopsy Method: A different way of doing LC. [Re: rockyfungus]
    #27423508 - 08/11/21 01:05 AM (1 month, 6 days ago)

Quote:

rockyfungus said:
Anyone else loving the poke for transfers even?

From:

To:

So, yeah, been fighting green all summer, and without the Poke I would of burned the whole house down.




Now there's an impressive advertisement for this Tek. Well done and I like the agar Tupperware containers as well.


--------------------

Anything written above is just as likely to be accurate as it is to be corrected by someone smarter than me two posts from now.


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Offlinerockyfungus
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Re: The Biopsy Method: A different way of doing LC. [Re: karri0n]
    #27423753 - 08/11/21 08:58 AM (1 month, 6 days ago)

Dual comparison of transfers! With such a huge area to work in, if your technique is bad your see exactly which way your arm came over the huge sandwich box.

I should say left hand of box was a blade, right hand was "poked"


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Team frugal


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OfflineSo Anyway
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Re: The Biopsy Method: A different way of doing LC. [Re: rockyfungus]
    #27425447 - 08/12/21 04:29 PM (1 month, 4 days ago)

How do these LC's look? After ~2 weeks untouched

Grain water broth:

Stirred


Settled


Control


Powdered grain broth:

Stirred


Settled


Control


--------------------
So anyway, uh...

introduction thread


Edited by So Anyway (08/12/21 04:30 PM)


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Invisiblepablokabute
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Re: The Biopsy Method: A different way of doing LC. [Re: rockyfungus]
    #27427255 - 08/13/21 10:10 PM (1 month, 3 days ago)

Quote:

rockyfungus said:
Anyone else loving the poke for transfers even?

From:

To:

So, yeah, been fighting green all summer, and without the Poke I would of burned the whole house down.






I would of burned the whole house down.


I know what that feels. My mushcult shed recently got flooded with heavy rains and contaminant spores are everywhere despite working in a SAB armed with intermediate techniques.

I might just burn my entire shed down pretty soon if I dont control this setback.


--------------------

Fermented Mushrooms!!
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