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OfflineSeamonkey84
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Registered: 03/26/22
Posts: 506
Last seen: 13 days, 1 hour
Re: Josex' Poke: A different way of doing LC. [Re: altford78] * 1
    #28356602 - 06/12/23 06:49 AM (7 months, 13 days ago)

The one syringe of sterile water is all you need. The mycelium and contamination will all be in the needle, which you flame sterilize between pokes. You only ever eject water out, do not suck any air or anything up into the syringe. Also, let the needle cool on its own, don’t squirt water out to cool it, it’s better to do the poke with a dry needle.


Edited by Seamonkey84 (06/12/23 06:51 AM)


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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: Josex' Poke: A different way of doing LC. [Re: Seamonkey84]
    #28357242 - 06/12/23 03:14 PM (7 months, 12 days ago)

Thanks man. Yeah, my brain was just fried and I was having these concerns about myc instantly traveling everywhere lol. I knew it was bullshit but I couldn't be bothered to deal with my own shit so I just cooked a few more syringes. :babypalm:

Yeah I wasn't too happy with the wet needle, kept forgetting to shake that last drop off before poking. How do you know if it's cool though, I'm in a sab so there's no airflow. Just count like 20seconds?


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Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes


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Offlinetittlez
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Registered: 11/25/18
Posts: 161
Loc: Europe
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Re: Josex' Poke: A different way of doing LC. [Re: altford78]
    #28410118 - 07/27/23 09:49 AM (5 months, 30 days ago)

To start off I've never done LC but have 20 jars that need to be inoculated soon and not enough agar plates ready to do the job..
So I made some broth (500 ml with 1g LME - split into 2 jars) but forgot to sterilise a syringe with water in it. Now I'm wondering if I could just take a sterile syringe and needle from the farmacy and suck up a little of the broth, flame the needle, let it cool and then poke? Or is that not a good idea?

Aside from that.. how long would I need to let the LC sit before I could use it to inoculate my 20 rye berry jars?

If this is a rubbish idea I guess I'll just let the grain sit for 2 weeks whilst I wait for more agar plates. Or just inoculate a few jars and then G2G.

Any thoughts?


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Josex' Poke: A different way of doing LC. [Re: tittlez]
    #28410132 - 07/27/23 09:56 AM (5 months, 30 days ago)

have you considered doing a LI with what you do have?


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinetittlez
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Re: Josex' Poke: A different way of doing LC. [Re: Camera93]
    #28410179 - 07/27/23 10:34 AM (5 months, 30 days ago)

Can you recommend a TEK? Im having a hard time finding something i can do without special equipment that i dont have.

I am going to sterilize some grain now and alternatively could throw in a syringe with some distilled water. Just not sure about PCing it for 90 minutes


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Josex' Poke: A different way of doing LC. [Re: tittlez] * 1
    #28410184 - 07/27/23 10:38 AM (5 months, 30 days ago)

Quote:

tittlez said:
Can you recommend a TEK? Im having a hard time finding something i can do without special equipment that i dont have.





Blenderless Liquid Inoculate

In a nutshell:
Sterilize a jar of water, drop your culture into the jar, shake the shit out of it and pour over your receiving grain jars.


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinetittlez
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Registered: 11/25/18
Posts: 161
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Re: Josex' Poke: A different way of doing LC. [Re: Camera93]
    #28410203 - 07/27/23 11:00 AM (5 months, 29 days ago)

Quote:

Camera93 said:
Quote:

tittlez said:
Can you recommend a TEK? Im having a hard time finding something i can do without special equipment that i dont have.





Blenderless Liquid Inoculate

In a nutshell:
Sterilize a jar of water, drop your culture into the jar, shake the shit out of it and pour over your receiving grain jars.




Love it :heart: Will definetely give that a try. Just threw in a jar with water with my grain :dancer:

Thanks a lot for your support!

:yourock:


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OfflineAntares12
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Registered: 07/14/23
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Re: The Biopsy Method: A different way of doing LC. [Re: tittlez]
    #28417407 - 08/02/23 12:08 PM (5 months, 23 days ago)

Very valuable knowledge. Thank you for your post


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OfflineBustaboo
Calmer than you are


Registered: 07/17/23
Posts: 118
Last seen: 12 days, 24 minutes
Re: The Biopsy Method: A different way of doing LC. [Re: Antares12]
    #28428371 - 08/10/23 12:23 PM (5 months, 15 days ago)

Looks a lot easier than agar wedges. I look forward to trying this out.


--------------------
Looking for Yeti and Ghost spores.


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Offlinetittlez
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Registered: 11/25/18
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Re: The Biopsy Method: A different way of doing LC. [Re: Bustaboo]
    #28432422 - 08/13/23 02:05 PM (5 months, 12 days ago)

Here's my first attempt at LC with this TEK. Seems like I have some nice growth. I'm wondering if and when I should swirl the LCs to try to break up the myc? Also how to test the LCs without risking the cleanliness again? Can I just poke through the polyfill filter and suck LC up and onto agar? I would use a syringe and needle that are sterile out of the packaging. Does it still make sense to flame the needle to avoid contaminating the needle whilst piecing through the polyfill filter?



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OfflineBustaboo
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Registered: 07/17/23
Posts: 118
Last seen: 12 days, 24 minutes
Re: The Biopsy Method: A different way of doing LC. [Re: tittlez]
    #28432452 - 08/13/23 02:32 PM (5 months, 12 days ago)

You might check out this tek as well:

https://www.shroomery.org/forums/showflat.php/Number/26022486/vc/1

It says to swirl several times a day. To test to agar, use an inoculation loop and tip the jar.


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Looking for Yeti and Ghost spores.


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Offlineshroomshine
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Registered: 03/11/20
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Re: The Biopsy Method: A different way of doing LC. [Re: Bustaboo]
    #28434781 - 08/15/23 09:52 AM (5 months, 11 days ago)

If you have time I recommend testing on grain. LC test plates are hard to interpret sometimes since the growth is unorganized and bacteria and or mold can be hard to recognize. Just my opinion…


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Offlinetittlez
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Registered: 11/25/18
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Re: The Biopsy Method: A different way of doing LC. [Re: shroomshine]
    #28434828 - 08/15/23 10:53 AM (5 months, 11 days ago)

Thanks for the tipps!! I guess I'll give both a try. Although honestly I'm not sure I will do a better job at spotting the possible bacteria on grain. Time will tell :grin:

So to get some LC to grain is it best to pull some out with a sterile syringe or better pour it?

Also do I need to shake the LC whilst it's colonising? And also best to shake before using to inoculate?


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OfflineSandbar Hopper
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Registered: 07/28/23
Posts: 7
Last seen: 18 hours, 52 minutes
Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #28483515 - 09/26/23 10:08 AM (4 months, 48 minutes ago)

I inoculated 8 pint jars on the 18th (8 days ago) and they are looking pretty good. I used LME and there was just a bit of sediment in the jars at first and some of the "jelly fish" picked up some of that I think.



Does this look ok?

Imgur video


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OfflineAuto
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Registered: 07/07/23
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Last seen: 1 month, 8 days
Re: Josex' Poke: A different way of doing LC. [Re: Josex]
    #28553125 - 11/22/23 06:07 PM (2 months, 4 days ago)

I hope this works injecting LC to LC too! I'm giving it a shot thank you!


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Offlinefraki58
Mycology Learner


Registered: 11/20/19
Posts: 245
Last seen: 4 days, 21 hours
Re: Josex' Poke: A different way of doing LC. [Re: Auto]
    #28609142 - 01/04/24 06:13 AM (24 days, 3 hours ago)

Trying LC for the first time with the poke using a Pan Cult, looking clear af.



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If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best :smile:


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