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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mackload] * 2
    #29613317 -

It's called the candlesnuff fungus for a reason... :shrug:


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Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 4
    #29613318 -

Nichrome said:
It's called the candlesnuff fungus for a reason... :shrug:


Seriously?  :lol:  that’s hilarious

idontknow1 said:
fahtster said:
This is what I believe to be the first 100% clean pan culture I’ve had in the last 8 months and possibly ever.  It’s a clone that took a 20 minute syringe filtered h2o2 soak and a trench to get it.  No brown shit or biofilm.  Just myc and agar.



1% MEA.


Could you explain a ‘20 minute syringe filtered h202 soak’ just a bit?
Thank you:mushroom2:


It’s currently something I’m working out but basically, I take inner tissue and soak it in h2o2 (that’s been run through a sterile syringe filter) in a clean empty petri.  Then rinse it off in sterile water then add another drop or two of h2o2 from the syringe before putting it to a new plate.  So far it’s been working very well but there’s thing I need to work through before I properly present it.. which will probably be in my ziplock thread. 

These are early attempts that got the cultures close to clean


It’s not like it’s a new concept or anything but I haven’t heard of ppl filtering their h2o2 and using long soak times.  I tried unfiltered peroxide probably a year and a half ago and got pretty poor results but filtered long soaks are producing great results so far.  Things like, when you take the tissue sample in the fruits maturity, making sure the sample is white, and the amount you take all matter and part of what I’m working through

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #29613352 -

:takingnotes::rockon: anxiously waiting. More filtration teks always get me excited


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An enigma some caerulescens n a mono 😎

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #29613425 -

beautiful plates. your mention of filtered h2o2 puzzled me, so I started reading about purified peroxide. from what I read, regular drugstore hydrogen peroxide has chemical stabilizers like phosphoric acid, sodium stagnate, or acetanilide that leaves toxic residue. the article said that food grade h2o2 has none of these stabilizers and is pure. if I were anxious to attempt your strategy, couldn't I just use the food grade peroxide?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blakeyface] * 1
    #29613463 -

The filtering is to remove possible resilient endospores that may be in the h2o2.    When I tried peroxide in this way before, I got a lot of bacteria.  Granted, I didn’t do a long soak at all so, that probably played a role but when using the filtered h2o2, I don’t get any of that so it’s also just a “to be sure” the starting liquid is as clean as I can get it.  I’m also playing with peroxide agar and never sat well with me to add it to my sterile agar without filtering it first.  And the filters are like $1 so why not have the peace of mind, is my thought process


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #29613536 -

I am really anxious to try this as my cloning attempts thus far have been mediocre. I'm guessing Luer Lok? Pore size 0.22? or 0.45.? I appreciate your taking the time to explain this. Really looking forward to your ziplock thread too!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blakeyface] * 1
    #29613551 -

I use luer lock .22

Edit: I use an empty clean plate that’s slightly raised on the top edge towards the ffu face (I use a 60mm plate lid) so the peroxide pools on my side and use 5ml of peroxide.  Don’t squeeze it out through the filter too hard or you can break the membrane in the filter. 

I recommend that you clone an immature fruit as those have less cracks in their outer stipe and less time to become infected on the inner tissue and don’t take any tissue that’s not all white.. no blue spots or yellowing. 

After I let it soak for 20 mins, I’ll tweezer the piece into a 4oz jar of sterile water.  I do this to rinse off any dead cells that may be covering possible bacteria and then I kind of wipe the tissue on the dry surfaces of the empty part of the h2o2 plate to wick off extra water and add a couple drops of new h2o2 to the tissue while it’s sitting on a dry portion of the plate and let it sit for a couple minutes.  It should start bubbling again.  Then I repeat the wicking off of that h2o2 as much as I can before putting to a new plate.

For the first day, I’ll have the plate leaning almost vertical in case there is extra peroxide on the tissue.  This allows that excess to drain off to the edge of the plate.  If there is, I’ll dump that out in front of the hood.  I’ve gotten pretty good at wicking the excess off in the dry portions of the h2o2 plate so there hasn’t been a need to dump any out but my early attempts weren’t as “clean”

Edited by fahtster (06/29/26 11:19 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #29613571 -

Did this spore drag of a print I wanted to keep going, back 3rd of May. It sat blank until the last week or so, lower left is looking really clean and organized, but the scattered bit up to the right appears pretty clean, just scattered. Should I do transfers from both or concentrate on the organized lower left? What do y'all geeks say?

Edit- didn't notice until seeing the uploaded pic, seems the scattered area has some beige color going on. Hope that isn't something that's gonna smoke the plate.



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Edited by bluescreen (06/29/26 12:48 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #29613572 -

fascinating. thank you for the intricate feedback. I'm on my way

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen] * 1
    #29613585 -

Lower left would be my choice.


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk]
    #29613648 -

Kloakk said:
Lower left would be my choice.


Logical. But man I always root for the underdog!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen] * 1
    #29613704 -

bluescreen said:
Kloakk said:
Lower left would be my choice.


Logical. But man I always root for the underdog!


:whynotboth:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk]
    #29613776 -

Kloakk said:
bluescreen said:
Kloakk said:
Lower left would be my choice.


Logical. But man I always root for the underdog!


:whynotboth:


Why not both haha I haven't been able to do SAB work for a week (would get divorce papers if I turn off the fans)- but it's cooled off, maybe tomorrow morning, transfers all over (got some overgrown plates too).


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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen] * 1
    #29613799 -

If there's a will there's a way xD I have had success doing gar work in open air LOL you got this


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An enigma some caerulescens n a mono 😎

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen] * 2
    #29613904 -

bluescreen said:
Why not both haha I haven't been able to do SAB work for a week (would get divorce papers if I turn off the fans)- but it's cooled off, maybe tomorrow morning, transfers all over (got some overgrown plates too).


Could always ask the wife if it would be okay to buy a really expensive fan with a filter :awesomenod:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 4
    #29614229 -



TTBVI looks ready to go to grain, which I will be doing soon. I won't be around to b&s and check on my jars due to summer holiday stuff but oh well.



x7x+ does not want to be on this plate



Waiting for this one to jump but it's taking forever.



This plin clone should be clean but it's doing weird stuff. Should I send it or do more transfers?


Icebergs made the jump while I was away and they're a bit overdue for transfers.

I have a lot of work to do tonight


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Turvenuija]
    #29614232 -

I've always been confused by why ppl make stuff on agar jump to different mediums unless that medium piece is used to transfer to more gar to try to keep enzyme production if the medium is more similar or the same as what the spawns gonna be or the bulls gonna be. I've had great success going gar or lc to grains then using that grain to transfer to different spawn expansions or spawning it to bulk when doing this I've had quicker colonixation than if I tryed to force it to transfer or jump directly from gar in the petri to a diff substrate.?


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An enigma some caerulescens n a mono 😎

Edited by bloodssonnew (06/30/26 02:52 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bloodssonnew]
    #29614348 -

Can you rewrite that so that it makes sense please?

Are you asking why everyone doesn't do grain to grain?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #29614362 -

Well if u look in his agar plate he's got a toothpick or a cardboard strip I assume to have colonized to pickup n transfer easily without slicing as any other reason doesn't make sense to me. If trying to cleanup or avoid bacterial transfers cabin sequestering I'm 100% sure would be superior for the outcome if it's just about cleaning up.

And if he's doing it to start early enzyme production I'd think transferring early to grain then put colonized grain back to agar would result in more vigorous growth and stronger rhizos from my experience (can always be wrong and more than likely am lol)


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An enigma some caerulescens n a mono 😎

Edited by bloodssonnew (06/30/26 06:15 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bloodssonnew] * 2
    #29614367 -

It's a trench in his plate. And yes, it works like cabin sequestering.

You are actually right about taking colonized grains to agar though. That shit throws ropes.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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