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Re: Ask Quick Questions, Get Quick Answers [Re: Elint73]
    #29601861 -

A variety of???


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🅃🄴🄰🄼 🄸🄷🄾🄿


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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29601907 -

Thoughts? 8 days. One pic without and one with flash, same shoebox.




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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29601910 -

How do you store your cultures for extended periods?

I've seen so many different methods used here: liquid, with and without nutrients, on cardboard or simply agar plates?

But when you want to restart this—let's call it a master culture—how do you do that?

And is the procedure the same for active and gourmet mushrooms?


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resistance is futile you will be assimilated

:robawet:

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Re: Ask Quick Questions, Get Quick Answers [Re: wearetheborg] * 2
    #29601926 -

I use sterile water stasis LI for both active and gourmets. This tek uses a master and an inoculation sample for each culture to allow for nearly endless renewal before senescence.

It costs a bit more in supplies and space, but doesn't require refrigeration for extremely reliable long term storage and quick access to all of your genetics.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 1
    #29601982 -

Lookin good, Tropical!


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Re: Ask Quick Questions, Get Quick Answers [Re: flapping_bird]
    #29601998 -

Cheers bird, here some seeds to feed you!! :rofl:


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Stammgast]
    #29602020 -

Anyone?

Stammgast said:
I have spawned 3 tubs May 18, 19 and 25. 18 and 21 are giving me constant Fruits and I have harvested a lot already. These two are JDM. The third tub is True Natalensis and there is just not that much going on even though it seems the substrate is fully colonized.

Do they just take longer or do you guys see anything on the foto im missing?



JMF Results:






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Re: Ask Quick Questions, Get Quick Answers [Re: Stammgast]
    #29602039 -

If they are true natalensis and not ochraceocentra, then yes, they are reputed to take quite a long time compared to cubes. I have had several tubs fail to produce anything or even show any contam after several months. They colonized and then did nothing else. The rare fruits that I got were not hardy at all. That project has been moved to the back burner for me.

Have you cased them and if so, what was the casing? Also, check out the Nat thread for more specific recommendations. There are very few people growing true nats, so the information on indoor cultivation is pretty limited.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius]
    #29602040 -

Rasmodius said:
Moothy said:
Dear all hello,

ı did my cvg yesterday but i think it will be ready with low field capacity,can i add extra water without boiled and use instantly today ?



Yeah, pasteurizing coir alone isn't really a big deal. Any temp is fine. If boiling was gonna kill stuff, it's dead. Whatever's in the tap water, you were going to mist anyway.

Myth debunking thread



Also, you can leave out the v and the g. The v is unnecessary, and the g is pointless.

Way less hassle that way, and the environment will thank you.


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Re: Ask Quick Questions, Get Quick Answers [Re: wearetheborg] * 1
    #29602056 -

wearetheborg said:
How do you store your cultures for extended periods?




Poorly.

Every year, I say THIS is when I'm gonna get my shit together and do some proper long-term storage. But for the past few years, it's been more or less the same: let most of my pp5 agar containers just sit there and eventually dry out. Then rip out the crusty myc with tweezers and revive it on a new plate.

I did experiment a bit last year with making little grain masters and refrigerating them for like 4 months before expanding to quarts. Except I ended up leaving them in the mini fridge for 10 months before the next transfer. I don't recommend it, and I haven't spawned them yet, but so far so good. A dozen jars on deck waiting to be spawned, not the most amazing-looking but still OK, and another dozen from the next round of g2g transfers just chugging along and almost fully colonized.

The one thing that this comedy of errors has taught me is that multiple backups and multiple transfers is probably better than trying to meticulously preserve just a handful of cultures which aren't all that clean to begin with. My goal is still to accomplish both, though- someday.


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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29602074 -

Quote:
B Traven said:...
The one thing that this comedy of errors has taught me is that multiple backups and multiple transfers is probably better than trying to meticulously preserve just a handful of cultures which aren't all that clean to begin with. My goal is still to accomplish both, though- someday.



:whathesaid:
I still take prints, swabs and sometimes save some dried grains (not to mention the cupboards full of old plates). Just in case. I also just stick some cultures into cryo-vial stasis if I get overwhelmed with too many at once or need to take a break and the culture is not pristine yet. Always, belt and suspenders.

I save the LI tek for stuff that I am willing to commit some resources to, because they are good and clean and have proven themselves to be worthy for one reason or another.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #29602233 -

Cubes GT.
Kinda fucked up the shoebox top layer, some grains are open air. Is it an issue? Should I add coir and mist again?

Thanks


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wow

Edited by Tropical (06/11/26 07:50 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 2
    #29602248 -

How long since you spawned?

If like a day ago you COULD cover them. But if they were colonized then they won’t get contaminated anyways.

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29602269 -

Will be 24h in like 2 hours, don t have coir ready. Can I use coir with cold water, let sit for 2 hours, and cover shoebox? Or would you not bother?


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wow

Edited by Tropical (06/11/26 08:40 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 1
    #29602274 -

You can use cold water.

The mycelium won’t be won’t be fully recovered so why not.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29602328 -

Okay, that’s what I thought, was going 90 minutes instead of 2 hours after though.


Another question, soaked and 17.5 hours, threw it all on some cardboard, threw a fan on it with windows open on humid/rainy dy for like 14 hours.

Went out when I should have tended my grains.

Going to pressure cook tmrw and probably Sunday, had a 20 lb bag so it will take a couple rounds.

Am I fucked? Will they be too dry now? Last time they were too wet

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Re: Ask Quick Questions, Get Quick Answers [Re: Dj_karma_magnet]
    #29602351 -

What kind of grains? Try splitting a couple of them and check moisture content inside, dry on the surface is a good thing.


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 1
    #29602391 -

ReverendMyc said:
Quote:
B Traven said:...
The one thing that this comedy of errors has taught me is that multiple backups and multiple transfers is probably better than trying to meticulously preserve just a handful of cultures which aren't all that clean to begin with. My goal is still to accomplish both, though- someday.



:whathesaid:
I still take prints, swabs and sometimes save some dried grains (not to mention the cupboards full of old plates). Just in case. I also just stick some cultures into cryo-vial stasis if I get overwhelmed with too many at once or need to take a break and the culture is not pristine yet. Always, belt and suspenders.

I save the LI tek for stuff that I am willing to commit some resources to, because they are good and clean and have proven themselves to be worthy for one reason or another.



Thanks guys. The LI idea sounds great, especially the fact that it can be stored at room temperature. But not relying on just one method is probably the best option. Thanks.


--------------------
resistance is futile you will be assimilated

:robawet:

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 1
    #29602397 -

ReverendMyc said:
I use sterile water stasis LI for both active and gourmets. This tek uses a master and an inoculation sample for each culture to allow for nearly endless renewal before senescence.

It costs a bit more in supplies and space, but doesn't require refrigeration for extremely reliable long term storage and quick access to all of your genetics.



This is gold - thank you!

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Re: Ask Quick Questions, Get Quick Answers [Re: CoolBreeze06]
    #29602409 -



It's me again. This is my second shoe box. It's 16 days old. What do you think?

Unfortunately, nothing's happening. The lid is on the box. The surface actually looks fine in terms of moisture. Is it normal that nothing's happening yet? Should I just be patient? Or is there anything I can do to encourage pinning?


--------------------
resistance is futile you will be assimilated

:robawet:

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