bloodssonnew said:

There is a India woodlover that likes to fruit in warm temps ( gonna try to grow it eventually)

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mobyhat said:

I have agars like this..how many time in months I can store they:

1. out of the fridge at 23/25°C?

2. in the fridge at 6/7°C?

thank you!!

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Room temp agar 2+ years revival of chestnuts. I've done reishi lion's mane, shiitake, and cubensis after over a year.

SignedinStranger said:

Two Pan Cyan plates after transferring a tiny sliver of just the mycelium that was climbing up the wall of the petri dish. The purpose was to try to separate the mycelium from some sort of red/orange, emedded contam. Credit to Fahtster for the technique. It took a few days to see any visible growth but its looking good now. The MIB is looking the healthiest it has looked so far (over a span of 2 transfers and ~10 plates) so fingers crossed this worked.

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Quote:

It could be as simple as the reusable dishes you are using are losing their binding phenol through multiple pc cycles and becoming porous enough to let pseudomonad types through. This is almost identical to several friends issues recently all who were using those same reusable dishes, and all of them stopped having the issue occur when they switched dishes

Reusable plastic is made with phenolic binders that eventually denature and cook out of the plastic rendering it water tight but porous enough for bacteria to run through it.

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idontknow1 said:

@Faht…would this technique of grabbing the leading edge of the hyphae climbing the lip of a plate result in similar results to mycelium reaching up and growing on the ‘ceiling’ of the agar when the plate is turned upside down, with inoculating wedge on the lid?

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