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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BagShaker] * 1
    #29567666 -

Personally I’d let 1, 4, 5, 6 & 7 grow out a bit more and as long as they stay looking like they are I’d send them to grain. Before you drop them to grain take a transfer if you want to keep the culture going and/or take some samples to a cryovial.

On your 3 top photos you can literally take a transfer from 12 o’clock on any of the 3 plates.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 1
    #29567724 -

What is the most likely vector for satellite contamination? And I know the standard practice is to transfer away from contams, but what about transferring the contam out of the plate?

I assume if it is a viable option, transferring contam away from the plate is dependent on the type of contam and where it is located, and I feel like I have 2 plates that may be a candidate for this.

The first plate looks like some type of bacteria and the second plate may be yeast?




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Re: AGAR ENVY! (Anything and All things agar!) [Re: SignedinStranger] * 5
    #29567732 -

In my experience bacterial satellites comes from me or my tools. Take a small wedge to a new plate, trying to remove the bacteria is not worth the risk in my opinion.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 1
    #29567763 -

Is there bunk malt extract?

I've mostly used PDA and grain water agar, but a few months ago got some malt extract, as it's dead easy to prep sort of the standard, and it just gave weak-ass growth. Back to PDA and grain water, then decided to try malt extract again. 2% ME, 2% agar, "standard" recipe... weak-ass growth. The malt extract I can get around here is a sort of general brand you get at the drugstore, no brew shops or anything around. I did order some from a brewer supply house which should be here this week, will see if its' different.

Also poured 10 each plates at 1% and 3% and did a few transfers from a really nice thick rhizo PDA plate yesterday to see how that goes. 

Here's what I'm talking about, PDA plate on left.




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Edited by bluescreen (05/04/26 12:37 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bluescreen]
    #29567880 -




Okay should be first pic is a old spore print like year old and it looked rough then some white came out and a circle of white but whats looks clean for transfer
Along with pic number 2
Number 3 is a new print I know those yellow dots come from dirty spore will i get some white out it been maybe a week

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomxmush]
    #29567886 -

Number 3 on bottom is clone I havent been able to get a clean clone to turn mycelium if you can't see pic good first pic was a yellow circle that just started to grow some white out of it and bottom right pic 1 was a yellow circle but then a random white circle appeared I guess I can transfer from that? Idk

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomxmush]
    #29567890 -

Number 2 was yellow circles but now turning white and growing over the yellow I guess wait till past yellow circle and get a peace off it the 3rd is a clone and dirty spore idk if h202 will clean those yellow dots in dirty spore I watched a guy use some type antibiotic and it cleaned it up

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomxmush] * 1
    #29567906 -




These are all 2nd trans of pe i think gt or b+ and treasure coast but is the fluffy normal looking. And why do I have trouble on clone to agar I got a nice fruit I just picked of pe and it was in a bunch like 5 fruits all together so clone the bigger one right and hurry and go stright to agar or should I spray h202 on it I break them in half cause I read not to cut and get small sample from bottom

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomxmush]
    #29567911 -


This is crazy I had a old gt print i went to agar no good but let it go few days and this little white kinda bubble with a a flat side and just sitting in middle of jar i poured all dirty stuff out but u think I can put this on agar and cut a good cut

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #29568701 -

About 6 months ago I left no pours and started with legit petri dishes.  I love them.  I spent years trying every other method.

What I don't love is the amount of plastic waste it generates.

What does everyone do with used plates?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: John in WI] * 4
    #29568718 -

I use autoclavable petris so I can clean and reuse them


--------------------
what i've been growing

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Re: AGAR ENVY! (Anything and All things agar!) [Re: arlo.arlo] * 1
    #29568896 -

I clean them and put them in my plastics recycling container.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: arlo.arlo] * 1
    #29569009 -

arlo.arlo said:
I use autoclavable petris so I can clean and reuse them


You know what. I need to do this!


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Favorite Teks

  ]

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Splittinghairs] * 12
    #29569147 -

I ve finaly did it folks. 20 transfers, 0 contam a week later !


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wow

Edited by Tropical (05/06/26 11:19 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tropical] * 1
    #29569194 -

:airguitar:
Nice!


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] * 4
    #29569214 -

Yea it s not mush but it s a good step for me :rofl:


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wow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tropical]
    #29569263 -

My strongest-looking candidates for T2s



First time with tamps, so I'm not totally sure if this is the typical growth for them (ATL7x = ATL#7 crossed with another variety)


--------------------
Feeling lost here? We get it. Here's the most up-to-date guide as of 2026.


I know I'm a fool, but that's why I keep trying to learn.
:peace::heart::mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Splittinghairs] * 2
    #29569850 -

I use autoclavable petris too (Vabiooth brand on amazon), and I sterilize them in a pp5 plastic yogurt container alongside the agar jar. They're working great for me, like a dozen reuse cycles and counting.


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Giveaway: Six Sides of the Cube Low-Spore "Black Cap" Ochra wedges

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tropical]
    #29569861 -

Tropical said:
Yea it s not mush but it s a good step for me :rofl:


It will be soon!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: pickle_jars] * 2
    #29569877 -

pickle_jars said:
I use autoclavable petris too (Vabiooth brand on amazon), and I sterilize them in a pp5 plastic yogurt container alongside the agar jar. They're working great for me, like a dozen reuse cycles and counting.


Another happy Vabiooth user here. And many more can be found here: https://www.shroomery.org/forums/showflat.php/Number/28744852/fpart/1/vc/1 so highly recommended! Personally I sterilise them in these: https://www.ikea.com/gb/en/p/eklatant-jar-with-lid-clear-glass-bamboo-30419058 (17 per jar).

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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