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Re: Ask Quick Questions, Get Quick Answers [Re: stagger]
    #29510193 -

I got a couple questions:

1. What’s the consensus on how long to dehydrate? I’ve been running my dehydrator at 165f for 16-24 hours and I’ve always thought that was a bit much but I thought I remember reading that that’s the way to do it. With electricity as expensive as it is rn I’m looking to cut down on usage and if I don’t need to run it that long that would be amazing. Any tips about dehydrating and how long it usually takes you fine folks to get that glorious “cracker dry” benchmark?

3. What do y’all think about using smash wedges as long term culture storage? I’ve read they can keep for a long time, and I was even thinking about putting them in the fridge to keep longer.

Thanks in advance :rockon:

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Re: Ask Quick Questions, Get Quick Answers [Re: ElGringoGrande]
    #29510196 -

1.  It really depends on how dense the mushrooms are and how full the dehydrator is.  Tiny little pans I dry sometimes for only four hours :shrug:  Something thick like PE I probably would cut the mushrooms in half and do 24.

2.  I've been thinking about this too.  At least for short term storage while I test a culture.  Smash wedge in the fridge.  Water agar might keep them longer too.  But I haven't experimented with it yet.  Probably will soon though.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 2
    #29510205 -

I run a minimum of 24 hours, maximum of 48. I.e., I go for the minimum 24, then get around to emptying the dehydrator sometime within the next 24. Part of that is just because 48 hours is the maximum amount I can set the timer for. And I also pull all my fruits apart to inspect them, so there's lots of surface area.

I don't know nothin about no consensus, but I'd definitely advocate for that bare minimum of 24 hours. A lot of people don't understand what truly dry mushrooms look and feel like. They should basically tirn to dust if you rub them between your fingers, and snap into shards if you bend them.

The problem with asking about any long-term storage technique is that it takes a very long time to test it. And smash wedges are just now gaining traction. I'd imagine that it would work pretty well, though.


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ACAB  🖕🧊


Edited by B Traven (03/09/26 12:53 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29510241 -

Agar in baggies gets really dry (like a sub-millimeter thick sheet of brittle seaweed) over time. The plastic film and/or the zipper mechanism lets out all the moisture. I can't remember whether it was 6 months or about a year in the desk for me before that happened, def not more than a year. I had mixed success reviving and it was a huge pain to handle (I'd get a little chunk out and the FFU would blow it right away - might have been less awful in a SAB). Edit: Probably worth noting that this was mailed to me. If you put a wedge into a bag yourself and didn't mail or roll it completely flat, it would at least be a bit thicker even if it did dry out.

OTOH I've revived distilled water cryovials from 18 months ago consistently. (Though, someone I mailed some this old to to had trouble getting them to grow - not sure if the mail stressed it out too much or if they would have failed for me here.) Sometimes it can take a week or more for a wedge stored this way (especially for a long time) to start showing any sign of life, definitely longer than a wedge from a refrigerated plate, so be ready for that.

LC stored in cryovials at room temperature also seems to revive great after over a year (but I've only done it a few times). Maybe even better than the wedges. I usually don't put LC in syringes, so getting it into cryovials doesn't easily fit in my workflow. But I bought some long blunt needles recently to try pulling it directly out of my 250 ml media bottles without much contamination risk (previously I'd pour into a smaller sterile jar first, but less stuff to sterilize would make me more likely to actually do it).

Edited by Phaethon (03/09/26 01:55 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29510255 -

B Traven said:
I run a minimum of 24 hours, maximum of 48. I.e., I go for the minimum 24, then get around to emptying the dehydrator sometime within the next 24. Part of that is just because 48 hours is the maximum amount I can set the timer for. And I also pull all my fruits apart to inspect them, so there's lots of surface area.

I don't know nothin about no consensus, but I'd definitely advocate for that bare minimum of 24 hours. A lot of people don't understand what truly dry mushrooms look and feel like. They should basically tirn to dust if you rub them between your fingers, and snap into shards if you bend them.

The problem with asking about any long-term storage technique is that it takes a very long time to test it. And smash wedges are just now gaining traction. I'd imagine that it would work pretty well, though.



This.

Just always 24 hours. Safer than sorry. When I was a noob noob I tried to get it out fast enough and couple months later the jar was soft.

Once I understood there was no such thing as too long, just set it for 48 and when I got to them after 24 I got to them

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29510258 -

I appreciate you sharing your experience!

I am still going to try it, but I will store the baggies inside of a small jar to help with moisture loss.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29510365 -

Great idea! I bet that would be pretty effective. Love to hear how it goes.

General advice for people getting into storing cultures: Cataloging for finding what you want a year or more from now is also something to consider before you have too many around. :smile: I went overboard and wrote a computer program (which I also intended to use for logging grows, but haven't actually followed through with that). But a spreadsheet with some way to note the location would work. With cryovials I use numbered storage boxes and the "grid" coordinates within the box (e.g. 2-D7). With small jars you could probably just note the jar number and manually look through the bags or vials stored in the jar.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29510392 -

Phaethon said:
Great idea! I bet that would be pretty effective. Love to hear how it goes.

General advice for people getting into storing cultures: Cataloging for finding what you want a year or more from now is also something to consider before you have too many around. :smile: I went overboard and wrote a computer program (which I also intended to use for logging grows, but haven't actually followed through with that). But a spreadsheet with some way to note the location would work. With cryovials I use numbered storage boxes and the "grid" coordinates within the box (e.g. 2-D7). With small jars you could probably just note the jar number and manually look through the bags or vials stored in the jar.



Nice! With 2ml vials I write a number on the top of the lid and log it into an excel spreadsheet with the exact contents and date it was vialed an the date the plate was made and what plate it was. With larger vials like 5ml & 20ml I print a label with all the relevant information and put it on the side of the vial.

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29510422 -

Tried something new, put millet in jars 1:1 with water and then pressure cooked.

They turned into sticky gelatin and I lost the whole batch and wasted hours with the grain prep and cook.

Any idea why this happened? I was excited to try the no soak no boil thing and I'm rather disappointed

Also I was trying my hand at grain bags because I ran out of jars and my vacuum sealer melts through the plastic. Lost three bags fucking around with that. . Still have 2, is it possible to just rubber band them closed or something instead of sealing the top bit?

Boo bad cult day


--------------------
- My trade list
- New here? Me too! Pop by and say hi c:

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Re: Ask Quick Questions, Get Quick Answers [Re: Archespore]
    #29510430 -

I just did some but with half the amount of water. They didn't turn out too bad but not great either. I'm gonna mess with it a bit. My buddy told me his usual recipe, which is 1:2, water:birdseed. I think it could've hydrated better but it wasn't soaked, or super slimey. Couple clumps.


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Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean]
    #29510461 -

I suppose it could've been too much water. I was worried that I translated something wrong and got the millet equivalent of like ... Hulled oats, like human kind you know?

Maybe it's a sign from the gods that I shouldnt be lazy and just simmer the damn grains lol


--------------------
- My trade list
- New here? Me too! Pop by and say hi c:

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Re: Ask Quick Questions, Get Quick Answers [Re: Archespore]
    #29510473 -

I get what you're saying about the wrong grains. I had to check on here to see if I got something worth messing with.

When I did it it at those ratios, it seemed like the bottom cooked way more than the top, but I also left the lids loose, as was part of my friend's directions. I'm thinking maybe I vented a bunch of water out that could've cooked the top. If I get it to work nicely I'll pm you with a tek.

I'm gonna keep trying this though. I don't think I've had a grain come out as nicely dried on the outside the first time I ever used it as the birdseed came out. Did you rinse the ever loving fuck out of your wbs before you pc'd it?


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Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29510498 -

ThurmanMurman said:
If I want to use grain water from grain prep to make my agar, how does one calculate how much GW to use in the agar recipe?

Edit:I'm seeing 50% GW and 50% straight water for agar. What say ye?



Inconsistency is why I never use grain water. I never use an identical amount of boil water to begin with


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Archespore] * 1
    #29510522 -

Archespore said:
Tried something new, put millet in jars 1:1 with water and then pressure cooked.

They turned into sticky gelatin and I lost the whole batch and wasted hours with the grain prep and cook.

Any idea why this happened? I was excited to try the no soak no boil thing and I'm rather disappointed

Also I was trying my hand at grain bags because I ran out of jars and my vacuum sealer melts through the plastic. Lost three bags fucking around with that. . Still have 2, is it possible to just rubber band them closed or something instead of sealing the top bit?

Boo bad cult day



250ml millet
125ml water

Shake immediately after depressurization

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29510524 -

I know you're not replying to me, but thanks! I'll try those measurements next time I do some wbs!


--------------------


Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean]
    #29510528 -

Yeah no worries.

I’ve been doing no soak for a bit. Love it!

I don’t rinse my millet. Straight into jar. Add water. I put a dash of gypsum on top.

The “worst” part about it is the day after cooling I flip it upside down for a day to let any remaining moisture soak in.

I have pics in my yeti and if clone grows



EDIT same jar 4 days after BnS


Edited by Splittinghairs (03/09/26 07:11 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29510766 -

ThurmanMurman said:
If I want to use grain water from grain prep to make my agar, how does one calculate how much GW to use in the agar recipe?

Edit:I'm seeing 50% GW and 50% straight water for agar. What say ye?



I just added the normal amount of agar to the grain water without diluting it.  Worked great.  :shrug:


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Ask Quick Questions, Get Quick Answers [Re: andymc]
    #29510785 -

What’s going on with this plate ? This is from a lc I put on agar after some bad tubs

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Re: Ask Quick Questions, Get Quick Answers [Re: Sealifefungi] * 1
    #29510793 -

Sealifefungi said:
What’s going on with this plate ? This is from a lc I put on agar after some bad tubs



It looks like there's a lot going on on that plate matey.

Were you spraying that LC all over the place, or did you invite a whole lot of guests to the party?


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Ask Quick Questions, Get Quick Answers [Re: andymc]
    #29510957 -

Honestly I haven't had good airborne humidity from water tubs. I still recommend and use the Perlite SGFCs.

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale


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