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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29482432 -

I just checked out out the agar dishes that I used to transfer into the grain and sure enough 2/3 got the same foul smell as the jars and a slightly yellowish discoloration - a noticable contrast to the one dish which apparently is not infected.
It's premade agar, so maybe it already was infected. At least I still got one non infected plate.


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

Edited by brominegeek93 (02/12/26 02:57 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 2
    #29482445 -

When I first started doing agar in 2024 I bought premade agar petris from two sources.  I let them sit unused for a couple months, because I had decided to just give agar a go myself after reading a few threads that made it sound achievable for beginners.

Every single plate from both sources showed contamination when I took them out of storage to eventually use them.  Buyer beware on ordering pre-made plates.

Agar thread that got me started:
Pastywhyte's Easy Agar Tek

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Re: Ask Quick Questions, Get Quick Answers [Re: Thankful]
    #29482447 -

and here I thought I could take a shortcut


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 3
    #29482450 -

BeefExtreme said:
tultedspoon said:
I know a lot of people are using agar for their grows. To achieve reliable flushes and good grows, are most of the epic grow photos on here from a cloned fruit using agar?

Is anyone getting good flushes and grows from spores on agar? Or does all the agar work and transfers somehow achieve mycelium that is more ready to fruit? Is this why agar is recommended moreso than just for clean and non-contaminated samples?

Like maybe doing a couple transfers, that small amount of mycelium hits the growing time needed for it to be more ready to switch to fruiting conditions, then if you spread that agar to some jars or grows, you will end up with way more surface area of mycelium that is ready to fruit? Does this make sense or am I wrong?


this isnt at all a qq.  theres entire threads about it, namely the ones i linked in the hhg but ill see if i can give you a qa.

depends on the spores and how stable the parent culture was. 

heres an analogy:


lets say i grow a tub from a spore syringe.  that syringe might have more genetic differentiation in it than the country of china.  maybe you get lucky and we see some dense citys.  some skyscrapers.  maybe some apartment complexes.

more often youre going to see some rural farming communities. 

but then we clone from one of those dense cities and put it on a plate.  now you have the entire genetic sequence of the city of beijing.  yes, very dense, but every city has its suburbs and even ghettos of shanty shacks and houses.

so now we clone only the dense clusters of skyscrapers.  okay, now we have culture with a high propensity to fruit. 

keep doing this and eventually youll get a culture that just wants to make pure sky scrapers.

does that make sense?


sorry to do this, but i took a lot ot my personal time to answer this question without an acknowledgement from tulted.  just want to make sure he sees it..


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Thankful]
    #29482463 -



inoculated with live culture 01/29/2026, it's currently on day 14,
What do you guys who grow Lion's Mane think?

I'm assuming this is a fast 10-14 day strain and I can now throw it to my substrate blocks I made.

I'm thinking 2 1/2 cups of spawn (a jar worth) to each 5LB block. Toss a grain on agar to maintain the culture and make liquid culture soon.

Any tips for loading the grow bags, and how to leave them and what to watch for next!? :laugh:


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Re: Ask Quick Questions, Get Quick Answers [Re: Thankful]
    #29482481 -

guess I should've just bought a liquid culture for a start


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29482499 -

brominegeek93 said:
guess I should've just bought a liquid culture for a start


Nah, don't get discouraged!  It's all part of learning the process. Getting LC from a trusted vendor can be a shortcut, but learning the process is worth it. You get much better control over your cultures and can evolve/preserve good genetics when you get them (at least in theory - I'm not there yet lol).

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Re: Ask Quick Questions, Get Quick Answers [Re: Thankful] * 3
    #29482517 -

Thankful said:
brominegeek93 said:
guess I should've just bought a liquid culture for a start


Nah, don't get discouraged!  It's all part of learning the process. Getting LC from a trusted vendor can be a shortcut, but learning the process is worth it. You get much better control over your cultures and can evolve/preserve good genetics when you get them (at least in theory - I'm not there yet lol).


And even then, trusted vendors can still be a crap shot.

I used to get a culture and inoc 4 jars and 2-3 plates. Of all the ones I’ve had, think maybe 3 cultures were clean clean. Others I’ve had the culture outrun what little contam was in there, others I was able to save with some G2G to get the spawn amount I wanted, others didn’t get anything til I cleaned up the agar.

If you’re new new, there is nothing more rewarding than learning all the steps, taking spores to agar, colonizing grain and then rocking a full tub of your fruits.

I say this as someone with a lot of failures under my belt from rushing processes and not paying attention to the details. Think it’s taken me to grow about 25 to finally have a haul that from a first flush that was above acceptable. And that was from starting at home made agar.

You’ll get there.

Unless you give up on yourself.


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: Thankful]
    #29482536 -

I'm aware that agar is inevitable at some point. But the guide you posted seems simple enough. Certainly not worth the trouble buying sketchy premade agar.

I might just buy some liquid culture for a quick grow anyways.
Just found out Zamnesia seems to sell all kinds of liquid cultures. Among them medicinal cultures like Reishi and Shiitake.
Apparently these species thrive on sawdust, which is quite expensive in comparison to grains.
Could they also be grown on grains?
What do you think of this birdfood millet?


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29482538 -

Well, if the jars are submerged, they wont get the same level of sterilization as steam itself. So I think I should raise the trivet up a few inches with some bolts right? thanks

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29482543 -

Which vendor did you buy the cultures from?
I haven't given up. I'm about to buy some agar agar. The one remaining plate seems quite healthy with rhizomes clearly visible.

The Tek uses these small "gladware" containers that aren't available in europe sadly.
Does this work without tape?
https://www.shroomery.org/forums/showflat.php/Number/19208976

Any experience with these chinese jars you can find on ebay?



--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

Edited by brominegeek93 (02/12/26 04:50 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29482546 -

Splittinghairs said:
Thankful said:
brominegeek93 said:
guess I should've just bought a liquid culture for a start


Nah, don't get discouraged!  It's all part of learning the process. Getting LC from a trusted vendor can be a shortcut, but learning the process is worth it. You get much better control over your cultures and can evolve/preserve good genetics when you get them (at least in theory - I'm not there yet lol).


And even then, trusted vendors can still be a crap shot.

I used to get a culture and inoc 4 jars and 2-3 plates. Of all the ones I’ve had, think maybe 3 cultures were clean clean. Others I’ve had the culture outrun what little contam was in there, others I was able to save with some G2G to get the spawn amount I wanted, others didn’t get anything til I cleaned up the agar.

If you’re new new, there is nothing more rewarding than learning all the steps, taking spores to agar, colonizing grain and then rocking a full tub of your fruits.

I say this as someone with a lot of failures under my belt from rushing processes and not paying attention to the details. Think it’s taken me to grow about 25 to finally have a haul that from a first flush that was above acceptable. And that was from starting at home made agar.

You’ll get there.

Unless you give up on yourself.


:whathesaid: for SURE


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29482558 -

brominegeek93 said:
Which vendor did you buy the cultures from?
I haven't given up. I'm about to buy some agar agar. The one remaining plate seems quite healthy with rhizomes clearly visible.
Can you recommend some alternatives to traditional agar dishes that don't require messing around with foil? thanks


I can not name the vendors per forum policies and I have cult pics up so sorry bout that. Just go through one of the vendors here for better results and anyone not listed here, expect to clean up the culture.

Kinda confused on what you mean by messing with foil?

I just use my recipe and put it in 500ml erlynemyer flasks and just cover the two tops with the foil. Other than that, I free pour in my SAB.

Pasty Pours seems to be the tried and true no hassle agar recipe around here. Ketchup cups

https://www.shroomery.org/forums/showflat.php/Number/19208976

Edit. Added Lord Pasty’s Tek link


--------------------
Favorite Teks

  ]

Edited by Splittinghairs (02/12/26 04:56 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29482564 -

brominegeek93 said:
Which vendor did you buy the cultures from?
I haven't given up. I'm about to buy some agar agar. The one remaining plate seems quite healthy with rhizomes clearly visible.

The Tek uses these small "gladware" containers that aren't available in europe sadly.
Does this work without tape?
https://www.shroomery.org/forums/showflat.php/Number/19208976

Any experience with these chinese jars you can find on ebay?




Just use small mason jars with your custom filter lids, they won't mold if you keep it sterile and are perfect for novices.

You can boil your agar solution over the stove and pour it into each jar and pressure cook it. When jars cool and agar jello-fies turn jar upside down on their lid to get any standing water off the agar, when it looks good maybe next day turn it so it can breath and you've got awesome plates.

For fun you can leave a jar out in the wild and see it never contaminates. Used this method for years, I still like it over petri sometimes.


--------------------

Edited by FounderZ (02/12/26 05:09 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29482571 -

brominegeek93 said:
Which vendor did you buy the cultures from?
I haven't given up. I'm about to buy some agar agar. The one remaining plate seems quite healthy with rhizomes clearly visible.

The Tek uses these small "gladware" containers that aren't available in europe sadly.
Does this work without tape?
https://www.shroomery.org/forums/showflat.php/Number/19208976

Any experience with these chinese jars you can find on ebay?




Not sure if those are PP5, but check out this thread featuring "holy grail" plastic containers for agar. Slightly less clear than glass, but they make agar much easier for many of us. Might need to look at more recent posts there to find a link to buy them, the earlier ones sold out on the 'zon.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29482574 -

Quote:
Kinda confused on what you mean by messing with foil?


I mean this petri dish wrapping tape. some folks here seem to just use saran wrap. But I wonder if this is absolutely nessecary to prevent contamination to enter.


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29482580 -

brominegeek93 said:
Quote:
Kinda confused on what you mean by messing with foil?


I mean this petri dish wrapping tape. some folks here seem to just use saran wrap. But I wonder if this is absolutely nessecary to prevent contamination to enter.


IME Cling rap is perfectly fine for wrapping dishes. A lot of people use parafilm but I think that's just a more expensive version of cling wrap. My understanding is that wrapping is mostly just preventing your dishes from accidentally getting knocked open. There may be some utility and helping keep them hydrated also but, by design, the petri dish prevents contamination just by virtue of the geometry of the top and bottom.


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Ask Quick Questions, Get Quick Answers [Re: Nokumana] * 4
    #29482595 -

I use cling for dishes. IME I am lazy or patient maybe or possibly both and parafilm tends to have issues over time as a dish wrap. It can crack or get little holes after some weeks or months and also seems be easily chewed through by fungus mites which are cosmopolitan.

I love it for a wide variety of laboratory uses, even dishes if I will use them soon. It's so useful in the lab though I wouldn't want to waste it on dishes these days though. Cling has the downside of reduced air exchange compared to parafilm though so with cultures that are atmosphere hogs cling can choke them out a bit.

As a culture we say, penny for your thoughts, and my two cents, which indicates that we are all a bunch of narcissists. We value our own opinions as double that of our peers.


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29482662 -

@ BeefExtreme

Appreciate it, sometimes I don't have time to get on here but I usually do go back and read through everything it just takes me awhile. Sometimes I log in and there's like 7 new pages I have to go through it sucks but is good because more to learn.

Thank you for the skyscraper analogy. At what point will spore prints produce skyscrapers? Would I have to struggle from spore prints for approximately 7 generations? Or would cloning a fruit, then hopefully growing a flush from that clone, then taking several different prints from that flush, then running separate prints in jars, expedite results by starting from a print that has maybe more spores with skyscrapers?

So I guess to ask is cloning necessary into getting skyscrapers into my spores? or is it always the standard 7 generations starting from spores each time that matters? Like maybe there is a print from the clone flush that would have skyscrapers after 3 generations?

Most of my jars look clean, only two have some orange metabolite drops pissing through the surface. Of the jars that look clean, I'm just having trouble understanding why things are taking forever to fruit. I have been trying to heat the room to low 70s, not sure if 75 degrees is an absolute must to get fruits or if fruits will just form slower if it's below that. I can't really increase heating due to costs. This is for Panaeolus. Some of the mycelium looks so good on the sides of my jar, way more than what was there on the shitty surface that gave me that fruit before.

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Re: Ask Quick Questions, Get Quick Answers [Re: tultedspoon] * 3
    #29482714 -

:stonedjerk::banbanban:


--------------------
Learn it, know it, live it


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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