flapping_bird said:

sure, that’s fine to do. If i were to do it, I would, inside a SAB or in front of a flow hood: by hand, tear the cake in half, top to bottom (or however it works out) and use a sterilized scalpel to take a small sample of mycelium from the freshly exposed interior of the cake and place that on agar.

Maybe do 2 plates that way. You’ll likely get a clean culture on agar that way. In a few days, if you start to see contamination on the plate (like wet goo), no worries, just take another transfer to a fresh plate and grow it out.

Good luck!

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Nichrome said:

Cakes are a very good skill to have with a lot of applications, but for culture work you want to practice your sterile technique ie agar, lc, and whatnot...

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ok dope thank u. i've got a pretty good handle on agar work, i might do cakes in the future but agar-oats-coir is good for me for now thxx

hidingbush said:

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Phaethon said:

I kinda doubt anyone in this forum can give you an actual reason. Life is analog, lots of environmental and genetic factors affect its behavior.

Re. clean print to grain, you can do it and if the print is clean you might have great success...but why take the chance on both wasting grain and not having a culture to go back to later?

(To be fair, some of the greats like fahtster do this or at least used to. Usually to small jars if I recall correctly, which are grown out in ziplocs to find interesting mushrooms to clone.)

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I been doing MS and it produces a wide variety of mushrooms. The T1 plate produced this sized mushrooms on the second flush my T2 plate got contamination and right on the thick rope part. I ended up transferring a smaller fuzzy growth away from contamination and ended up with this they where closer to the same size as each other, slighty more potent and slightly smaller.

I haven't taken many clones yet because I prefer playing with lots of cultures over improving one culture.

Im currently just deciding what cultures I like best I really like Panaeolus but find them tedious the small whispy mycelium is hard to tell if ready to spawn in grain and the severe bruising that doesn't fully go away is annoying.

Forgot to mention I got a strain on agar that still sectors mildly even though its an isolated cubensis strain. I don't understand MS vs Isolate mushroom but its interesting how the same strains has a wide variety of mushrooms.

CaptainTrips420 said:

Forgot to mention I got a strain on agar that still sectors mildly even though its an isolated cubensis strain. I don't understand MS vs Isolate mushroom but its interesting how the same strains has a wide variety of mushrooms.

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Arkyhog said:

Libertycap97 said:

Hey Shroomers,

How long would a quart of rye last in the fridge after its been sterilised?

Thank you kindly

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When stored in a modified jar with a filter or an unmodified jar with a secure lid, the contents can last for months, provided they are properly sterilized. Additionally, refrigeration is not necessary for long-term storage if you prefer not to use it. Personally, I keep oats on a shelf for months before using them. Just ensure that they are kept in a jar with a reliable lid, and you should be all set.

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I do exactly the same.  No fridge.  I make them in bulk and the site for months. Never have any issues.

Splittinghairs said:

hidingbush said:

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Just don’t get too upset or discouraged when you eventually go to wrap the parafilm around the plate and a transfer slides all around.  lol.

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I've had good luck with cutting into the receiving agar first and when I cut the transfer I push it down into that hole. It sticks right there, could be lack of many attempts but I've got good luck doing that!</font></font>

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Might have to give that a go.

I just usually pull too hard at the end of the parafilm wrap and the transfer slams around every blue moon. Lol</font>

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There are shrink wraps that will seal off petri dishes.  These are for 60mm dishes.  Place them over your petri dish, hit with hair dryer or heat gun.  It'll shrink around your dish.  No lateral forces will be applied to the dish causing the sample to slam around... Profit

https://a.co/d/0coYb8Yq

xhooliganx said:

Place them over your petri dish, hit with hair dryer or heat gun.

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callmehippie said:

If you put a cup of Coir in first and then pack it down it's still a cup of coir it doesn't matter if you packed it.

Just because it's packed down and now only measures to maby 1/4cup. In the container  The volume of is still the same.

100 lb of stones doesn't weigh more than 100 lb of feathers even if  the feathers can be packed in more.

It dosnt change.  I mean sure if you add more then it changes..


Stick to arkys and piggys advice it's golden.

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f

But the ratio is based on volume, right? Not weight. Sorry, but I'll switch to milliliters because I don't quite understand cups
If I'm supposed to use 1500 ml of feathers and 500ml of stones, my question is whether feathers should be loose or packed. 1500 ml of loose feathers will weigh significantly less than packed ones.